The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodes

Anastasiadis, I; Giannakou, Ioannis O.; Prophetou‐Athanasiadou, D. A.; Gowen, S. R.

doi:10.1016/j.cropro.2007.06.008

Cited by 104 publications

(66 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several methods known to manage the root-knot nematode include the use of nematicide, organic amendments, resistant cultivars, soil solarization and biological control, which have been used with different levels of success on tomatoes (Randhawa et al, 2001;Sakhuja and Jain, 2001). Although the application of chemical nematicide has been found as an effective measure for the control of nematodes, it has a high toxic residual effect on the environment and particularly on non-target organisms (Anastasiadis et al, 2008). In view of this, current research is focused on the development of alternative strategies that are environmently friendly and sustainable (Pinkerton et al, 2000;Mashela et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of some botanicals and Trichoderma harzianum against root-knot nematode (Meloidogyne incognita (Kofoid and White) Chit wood) in tomato

Feyisa¹,

Lencho²,

Selvaraj³

et al. 2016

J. Entomol. Nematol.

View full text Add to dashboard Cite

Root-knot nematode, Meloidogyne incognita (Kofoid and White) Chit wood) is one of the major constraints for the successful cultivation of tomato (Lycopersicon esculentum Mill.) in Ethiopia. Among different management strategies, biological control is important in the light of increased awareness of environmental and human health hazards. Therefore, the present study was conducted to evaluate the effect of different locally available botanicals and an antagonistic fungus, Trichoderma harzianum to control root-knot nematode attacking tomato under in vitro condition. Leaf and seed extracts of four botanicals viz., Rape seed (Brassica napus L.), Lantana (Lantana camara L.), African marigold (Tagetes erecta L.) and Neem (Azadirachta indica L.) at two different concentrations (5 ml and 10 ml) and Trichoderma harzianum (5 ml) were tested. Plant extracts were more effective and significantly inhibited egg hatching and immobilizing the J 2 larval mortality of M. incognita than T. harzianum. Aqueous extracts of all the botanicals inhibited egg hatching of nematode and resulted in 84.67 -100 % mortality of the second juveniles of M. incognita at the 10% concentration after 72 h of exposure time. Leaf extracts of T. erecta and A. indica exhibited 100% inhibition of egg hatch and larva mortality, while at similar concentration of B. napus and L. camara leaf extracts exhibited 92 and 93.2% inhibition of egg hatch and 62.1 and 73% larval mortality, respectively. Egg inhibition and larval mortality decreased with increase in the dilution (10 ml) of extracts. Juvenile mortality increased corresponding to an increased time of exposure. Aqueous seed extracts of A. indica more significantly inhibited egg hatching and larva mortality of the J 2 of M. incognita at 10% concentration and immobilized by 89, 93 and 100% after 24, 48 and 72 h of exposures, respectively, This study revealed that the test plants are readily available to farmers at no cost and able to reduce nematode population below economic threshold. There is a need for further studies in identifying new classes of bio-pesticides from natural plants to replace the synthetic chemicals used at present.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of some botanicals and Trichoderma harzianum against root-knot nematode (Meloidogyne incognita (Kofoid and White) Chit wood) in tomato

Feyisa¹,

Lencho²,

Selvaraj³

et al. 2016

J. Entomol. Nematol.

View full text Add to dashboard Cite

show abstract

“…), which are one of the most destructive nematodes, cause more than 10% loss of the world's total crop production each year (Anastasiadis et al 2008). Although chemical nematicides such as organophosphate and carbamate nematicides are highly effective in controlling nematodes, their usage has been restricted in recent years because of increasing concerns about their effects on the environment and food (Anastasiadis et al 2008;Kiewnick and Sikora 2006).…”

Section: Introductionmentioning

confidence: 99%

Enhancing the virulence of Paecilomyces lilacinus against Meloidogyne incognita eggs by overexpression of a serine protease

Wang

Liu

et al. 2010

Biotechnol Lett

View full text Add to dashboard Cite

To enhance the virulence of Paecilomyces lilacinus against Meloidogyne incognita eggs, a serine protease was overexpressed in P. lilacinus 9410 (PL9410). The cDNA of a mature serine protease gene was cloned from PL9410 and integrated into the genomes of PL9410 transformants through Agrobacterium tumefaciens-mediated transformation. Our results confirmed that the serine protease gene was overexpressed at the transcriptional level, and that the serine protease activities were enhanced in the transformants when compared to the parent strain. The bioassay results indicated that the relative parasitizing rates of the transformants against M. incognita eggs increased by about 20% in both conidial suspension and mycelium treatment groups.

show abstract

“…There are promising results with the use of P. lilacinus 251 (PL251) as a biological control agent against various plant-parasitic nematodes (Anastasiadis et al 2008;Yang et al 2011;Crow 2013). PL251 strain reduces infestation with M. incognita by nearly 66 % (Kiewnick and Sikora 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Commercial products of P. lilacinus are marketed in Europe, Central America and North Africa (Wilson and Jackson 2013). According to the common agricultural practices, P. lilacinus applications are combined with fungicide treatments to prevent secondary and associate infections caused by soilborne fungi (Anastasiadis et al 2008). Paecilomyces lilacinus 36-1 was isolated from M. incognita in our laboratory and showed effective control of root-knot nematode as well as soybean cyst nematode diseases.…”

Section: Introductionmentioning

confidence: 99%

Novel point mutations in β-tubulin gene for carbendazim resistance maintaining nematode pathogenicity of Paecilomyces lilacinus

2015

View full text Add to dashboard Cite

The application of fungicides is so critical, especially in greenhouses, to avoid fungal infections. Carbendazim, an inhibitor of tubulin biosynthesis, is the most widely known broad-spectrum benzimidazole fungicide. The application of carbendazim affects other beneficial fungi as well. Paecilomyces lilacinus 36-1 (Pl36-1) is a beneficial fungus used for biological control, and the most effective biocontrol agents of nematode eggs. The Pl36-1 is sensitive to carbendazim (0.3 μg/ml). There is a general consensus that the mechanisms of resistance to carbendazim in the β-tubulin gene have been analyzed in detail. However, no studies were conducted on P. lilacinus strains. In the present study, two carbendazim-resistant mutants of Pl36-1, P50 and P100, were obtained from UV exposure and tested. The β-tubulin gene fragments were cloned and sequenced in the three strains, Pl36-1, P50 and P100. The resistance to carbendazim was developed when amino acid substitutions occurred at β-tubulin gene positions of S145A, T185A and F200Y. The β-tubulin gene was overexpressed in Pl36-1 strains. The β-tubulin expression level of the overexpressed mutant (PL3), quantified by qRT-PCR, was increased 4-folds over its normal level in Pl36-1. In vitro, the PL3 was resistant to carbendazim with maintaining growth, sporulation and pathogenicity rates. Three-year field trial demonstrated that P100 and PL3 strains exhibited carbendazim resistance combined with high nematode reduction and yield improvement.

show abstract

The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodes

Cited by 104 publications

References 19 publications

Evaluation of some botanicals and Trichoderma harzianum against root-knot nematode (Meloidogyne incognita (Kofoid and White) Chit wood) in tomato

Evaluation of some botanicals and Trichoderma harzianum against root-knot nematode (Meloidogyne incognita (Kofoid and White) Chit wood) in tomato

Enhancing the virulence of Paecilomyces lilacinus against Meloidogyne incognita eggs by overexpression of a serine protease

Novel point mutations in β-tubulin gene for carbendazim resistance maintaining nematode pathogenicity of Paecilomyces lilacinus

Contact Info

Product

Resources

About