The Combined Effects of Bone Marrow–Derived Mesenchymal Stem Cells and Microporous Porcine Acellular Dermal Matrices on the Regeneration of Skin Accessory Cells In Vivo

Abstract

We investigated the effects of microporous porcine acellular dermal matrices (MPADM)-containing bone marrow-derived mesenchymal stem cells (BMMSCs) on accessory skin cell regeneration in vivo. Two kinds of the porcine acellular dermal matrices were prepared: one with microsized pores (the MPADM) and another without pores (the PADM). BMMSC populations from a Sprague-Dawley (SD) rat were seeded on both PADMs and MPADMs and cultured in vitro for 5 days. These rats were randomly divided into four groups: BMMSCs on an MPADM and covered with a PADM layer (group A), MPADM without cells and covered with a split-thickness skin graft (SSG) (group B), BMMSCs on an MPADM and covered with an SSG (group C), and BMMSCs on a PADM and covered with an SSG (group D). On post-surgery day (PSD) 5, all groups survived, except for group D. On PSD 7, there was no significant difference in the functional vascularization between groups A, B, and C. On PSD 14, large quantities of new capillaries, a larger rough endoplasmic reticulum in fibroblasts, and de novo unmyelinated nerve endings could be observed at the junction between the skin graft and the dermal matrix in group C; however, these structures were absent in groups A and B. The experimental results showed that MPADM could induce exogenous differentiation of BMMSCs in vivo and promote reconstruction of skin accessory cells.

Infrared Raman spectroscopy enables noninvasive biochemical assessment of skin tissue and the thermal stability

“…A healthy pig (25 kg, provided by the Animal Experimental Center of Wenzhou Medical University) was killed under proper ethical protocols. We obtained the pork belly and prepared the microporous porcine acellular dermal matrix (MPADM), which has been reported elsewhere [28] , [29] . In brief, the 0.3 mm-thick dermis was collected once the top 0.2 mm-thick portion of the skin was excised using a motor dermatome.…”

Regenerative therapy for the nonhealing cutaneous wounds

“…In this background, current research is focused on the developing of an artificial skin that could fully imitate a natural skin architecture and its functionality needed for the SCs transplantation. The cell-free biomaterials, e.g., a pig skin, amniotic membrane etc., were used to create a 3D-template optimal for SCs growth and differentiation [29]. Another approach is encapsulating SCs in hydrogels, which provides a true 3D system that supports several SC properties.…”