The Combined Effects of Hematoporphyrin Monomethyl Ether-SDT and Doxorubicin on the Proliferation of QBC939 Cell Lines

Liu, Liang; Xie, Sheng; Jiang, Lin; Jin, Hui; Li, Songgang; Liu, Jianwen

doi:10.1016/j.ultrasmedbio.2012.08.017

Cited by 39 publications

(31 citation statements)

References 24 publications

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“…The typical apoptotic characteristics of nucleus and high level of DNA fragmentation in U937 cells treated by SDT prompted that apoptotic death would happen when U937 cell were exposed to SDT treatment. A lot of studies on the anti-tumor effect of SDT hypothesize that the extrinsic death receptor pathway and the mitochondria-initiated pathway, which participate in cell apoptosis, might be involved in the malignant cells response to SDT [14,26,27]. More details about apoptosis in U937 cells under SDT treatment are still in further study.…”

Section: Discussionmentioning

confidence: 99%

“…The process of apoptosis induced by SDT may involve SDT induced damage on several cellular components, such as plasma membrane, mitochondria, nucleus and chromatin. On the other hands, some observations indicate that SDT may induce tumor cell apoptosis through the influence of genes regulating apoptosis [4,12,13,14]. However, since the efficacy of SDT may be influenced by multiple factors, including the nature of the biological model, the sonosensitizer, and the ultrasonic parameters, the detailed mechanism of SDT is unclear until now.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation

Wang

et al. 2014

Cell Physiol Biochem

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Background: Sonodynamic therapy (SDT) is an alternative strategy that manages malignancies via the generation of cytotoxic factors during ultrasound-activated sono-sensitive agents. However, the detailed mechanisms are not clear. This study was to identify the cytotoxic effects of ultrasound-activated protoporphyrin IX (PpIX) on U937 cells. Methods: Flow cytometry was performed to detect the time course for PpIX uptake in U937 cells. Sub-cellular localization of PpIX in U937 cells was visualized by inverted confocal laser scanning microscope. Following PpIX-mediated SDT treatment, cell viability was evaluated by the 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay; nuclear damage was observed under fluorescent microscope; DNA fragmentation and mitochondrial membrane potential disruption were measured by flow cytometry. The role of reactive oxygen species (ROS) in SDT-induced cell death was also evaluated. Results: We observed that PpIX is mainly localized in the mitochondria, with a maximal uptake within 2 h. Compared with PpIX or ultrasound alone, PpIX plus ultrasound treatment significantly declined cell viability, caused more serious damage of cell morphology, DNA and mitochondria. In the combined treatment group, the intracellular ROS was greatly higher than in other groups; ROS scavenger N-acetylcysteine could effectively rescue the loss of mitochondria membrane potential and cell viability induced by SDT. Conclusion: Taken together, these findings primarily indicated that fatal damage could be induced by PpIX-mediated SDT in U937 cells, and the intracellular ROS was involved during this process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation

Wang

et al. 2014

Cell Physiol Biochem

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“…Many sonosensitizers are derived from photosensitizers that have been used widely in photodynamic therapy. To date, many conventional photosensitizers, eg, hematoporphyrin, [14][15][16] Photofrin II, 7,17 ATX-70, 18,19 chlorin e6, 20 and ATX-S10, 21 have been confirmed to trigger cell death after activation by ultrasound. Therefore, development of photosensitizers may be an indirect way of identifying sonosensitizers.…”

Section: Introductionmentioning

confidence: 99%

Sinoporphyrin sodium, a novel sensitizer, triggers mitochondrial-dependent apoptosis in ECA-109 cells via production of reactive oxygen species

Wang¹,

Wang²,

Zhang³

et al. 2014

IJN

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Background Sonodynamic therapy (SDT) is a promising method that uses ultrasound to activate certain chemical sensitizers for the treatment of cancer. The purpose of this study was to investigate the sonoactivity of a novel sensitizer, sinoporphyrin sodium (DVDMS), and its sonotoxicity in an esophageal cancer (ECA-109) cell line. Methods The fluorescence intensity of DVDMS, hematoporphyrin, protoporphyrin IX, and Photofrin II was detected by fluorescence microscopy and flow cytometry. Generation of singlet oxygen was measured using a 1, 3-diphenylisobenzofuran experiment. A 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay was used to examine cell viability. Production of reactive oxygen species (ROS) and destabilization of the mitochondrial membrane potential were assessed by flow cytometry. Apoptosis was analyzed using Annexin-PE/7-amino-actinomycin D staining. Confocal microscopy was performed to assess mitochondrial damage and identify release of cytochrome C after treatment. Western blots were used to determine expression of oxidative stress-related and apoptosis-associated protein. Ultrastructural changes in the cell were studied by scanning electron microscopy. Results DVDMS showed higher autofluorescence intensity and singlet oxygen production efficiency compared with other photosensitizers in both cancerous and normal cells. Compared with hematoporphyrin, DVDMS-mediated SDT was more cytotoxic in ECA-109 cells. Abundant intracellular ROS was found in the SDT groups, and the cytotoxicity induced by SDT was effectively remitted by ROS scavengers. DVDMS located mainly to the mitochondria of ECA-109 cells, which were seriously damaged after exposure to SDT. Release of cytochrome C, an increased rate of apoptosis, and activated apoptosis protein were detected in the SDT group. In addition, relatively severe cell damage was observed on scanning electron microscopy after treatment with DVDMS and SDT. Conclusion These results suggest that DVDMS could be activated by ultrasound, and that DVDMS mediates SDT-induced mitochondrial-dependent apoptosis in ECA-109 cells via production of ROS.

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“…Comet assays were then performed as described in a previous study [23]. The slides were visualized by fluorescence microscopy with a 610 nm emission filter.…”

Section: Fluorescent Microscopy and Comet Assaymentioning

confidence: 99%

A novel class I histone deacetylase inhibitor, I-7ab, induces apoptosis and arrests cell cycle progression in human colorectal cancer cells

Yang

Liang

Shen

et al. 2015

Biomedicine & Pharmacotherapy

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The Combined Effects of Hematoporphyrin Monomethyl Ether-SDT and Doxorubicin on the Proliferation of QBC939 Cell Lines

Cited by 39 publications

References 24 publications

Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation

Cytotoxic Effect of Protoporphyrin IX to Human Leukemia U937 Cells under Ultrasonic Irradiation

Sinoporphyrin sodium, a novel sensitizer, triggers mitochondrial-dependent apoptosis in ECA-109 cells via production of reactive oxygen species

A novel class I histone deacetylase inhibitor, I-7ab, induces apoptosis and arrests cell cycle progression in human colorectal cancer cells

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