The comet assay in higher terrestrial plant model: Review and evolutionary trends

Lanier, Caroline; Manier, Nicolas; Cuny, Damien; Deram, Annabelle

doi:10.1016/j.envpol.2015.08.020

Cited by 44 publications

(24 citation statements)

References 160 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the in vivo studies also used cells obtained from a numerous different species including mainly rat, fish, mouse and worm. Some few studies also investigated DNA damage in bacteria (Danevcic et al 2016), plant cells (Cetinkaya et al 2016;Faisal et al 2016;Lanier et al 2015), amoebae (Kusrini et al 2016) or hydra (Zeeshan et al 2016). Whereas in the in vitro studies, a wide range of cell types belonging to different organ systems (e.g., A549, HCT 116, HEPG2, MCF-7, MRC-5, BEAS-2B, CHO-K1, and HEK293) were used, the in vivo studies were mainly based on cells from the blood system (about 61 %) like erythrocytes obtained from, e.g., fish or lymphocytes from, e.g., humans.…”

Section: Current Workmentioning

confidence: 99%

“…In addition, the data presented support that this technique may be a useful tool to complement conventional and -omics methods in situ environmental pollution monitoring. In consideration of 101 key publications which describe the use of comet assay in higher terrestrial plant models, it became clear that general consensus validates the use of the alkaline version of the test, the use of percentage of DNA in tail for measuring effects, and use of preferred roots to study (Lanier et al 2015). According to the collected data, 45 terrestrial higher plant species have been used for comet assay studies.…”

Section: Environmental Risk Assessmentmentioning

confidence: 99%

See 1 more Smart Citation

Comet assay: an essential tool in toxicological research

2016

View full text Add to dashboard Cite

The comet assay is a versatile, reliable, cost-efficient, and fast technique for detecting DNA damage and repair in any tissue. It is useable in almost any cell type and applicable to both eukaryotic and prokaryotic organisms. Instead of highlighting one of the numerous specific aspects of the comet assay, the present review aims at giving an overview about the evolution of this widely applicable method from the first description by Ostling and Johanson to the OECD Guideline 489 for the in vivo mammalian comet assay. In addition, methodical aspects and the influence of critical steps of the assay as well as the evaluation of results and improvements of the method are reviewed. Methodical aspects regarding oxidative DNA damage and repair are also addressed. An overview about the most recent works and relevant cutting-edge reviews based on the comet assay with special regard to, e.g., clinical applications, nanoparticles or environmental risk assessment concludes this review. Taken together, the presented overview raises expectations to further decades of successful applications and enhancements of this excellent method.

show abstract

Section: Current Workmentioning

confidence: 99%

Section: Environmental Risk Assessmentmentioning

confidence: 99%

Comet assay: an essential tool in toxicological research

2016

View full text Add to dashboard Cite

show abstract

“…Although, a large yield of nuclei can be obtained from protoplasts ( Gichner et al, 2008 ), the protocols for their isolation are available for a few species, mostly herbaceous, and they include different steps such as cell wall digestion, filtration, centrifugation, and separation of nuclei by density gradient centrifugation. In plant tissues, the SCGE technique is generally performed with a limited number of nuclei in comparison to animal studies, due to the difficulty to achieve a suitable amount of nuclei ( Lanier et al, 2015 ). This is particularly true for mature leaves, which are also very interesting for testing the effects of air pollution, as they are the primarily affected tissues.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Mesophyll Protoplasts from Mediterranean Woody Plants for the Study of DNA Integrity under Abiotic Stress

et al. 2016

View full text Add to dashboard Cite

Abiotic stresses have considerable negative impact on Mediterranean plant ecosystems and better comprehension of the genetic control of response and adaptation of trees to global changes is urgently needed. The single cell gel electrophoresis (SCGE) assay could be considered a good estimator of DNA damage in an individual eukaryotic cell. This method has been mainly employed in animal tissues, because the plant cell wall represents an obstacle for the extraction of nuclei; moreover, in Mediterranean woody species, especially in the sclerophyll plants, this procedure can be quite difficult because of the presence of sclerenchyma and hardened cells. On the other hand, these plants represent an interesting material to be studied because of the ability of these plants to tolerate abiotic stress. For instance, holm oak (Quercus ilex L.) has been selected as the model plant to identify critical levels of O3 for Southern European forests. Consequently, a quantitative method for the evaluation of cell injury of leaf tissues of this species is required. Optimal conditions for high-yield nuclei isolation were obtained by using protoplast technology and a detailed description of the method is provided and discussed. White poplar (Populus alba L.) was used as an internal control for protoplast isolation. Such a method has not been previously reported in newly fully developed leaves of holm oak. This method combined with SCGE assay represents a new tool for testing the DNA integrity of leaf tissues in higher plants under stress conditions.

show abstract

“…In turn, in the alkaline-alkaline approach, used in the majority of the studies owing to its greater sensitivity, breakages of single and double strands as well as alkali-labile sites and crosslinks are quantified. The choice of the comet assay version depends on the type of damage that shall be observed [38].…”

Section: Investigation Of Cell Death Mechanisms and Dna Damage Appliementioning

confidence: 99%

Cyto(Geno)Toxic Endpoints Assessed via Cell Cycle Bioassays in Plant Models

Andrade-Vieira¹,

Silveira²

2018

Cytotoxicity

View full text Add to dashboard Cite

Environmental pollution is a matter of great concern. Therefore, researches that aim to access the risk of toxicity of these potential pollutants are welcome in the scientific community. The most common strategy to detect toxic agents is through chemical analysis. However, in the last years, the biological assays are often important for risk assessments. Among the bioassays using living organisms to detect toxicity of a compound, plant models have been highlighted as it is easy to be conducted, has low cost, high sensitivity and presents good correlation with other test systems, including mammals. Besides, it is in accordance with the Toxicology Guidelines for the twenty-first century, which claims for bioassays that could substitute the ones that use animals as models. At cellular level, the cytotoxicity, genotoxicity, and mutagenicity are the parameters determined by the endpoints as mitotic index, DNA fragmentation, induction of cell death, and malfunction of cellular structures leading to chromosome and cell cycle alterations. Each of these endpoints will be presented in details in this chapter.

show abstract

The comet assay in higher terrestrial plant model: Review and evolutionary trends

Cited by 44 publications

References 160 publications

Comet assay: an essential tool in toxicological research

Comet assay: an essential tool in toxicological research

Isolation of Mesophyll Protoplasts from Mediterranean Woody Plants for the Study of DNA Integrity under Abiotic Stress

Cyto(Geno)Toxic Endpoints Assessed via Cell Cycle Bioassays in Plant Models

Contact Info

Product

Resources

About