The Comet Assay in Human Biomonitoring

Valverde, Mahara; Rojas, Emilio

doi:10.1039/9781847559746-00227

Cited by 3 publications

(3 citation statements)

References 152 publications

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“…The congruence of results between the comet assay and other endpoints such as micronuclei or sister chromatid exchanges (SCE), has been one of the principal reasons to increase the use of the comet assay as a biomarker for hazard assessment, particularly in monitoring the effects of occupational hazards [47][48][49][50][51][52].…”

Section: Comet Assaymentioning

confidence: 99%

Untitled

2017

AIMS Genetics

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Molecular epidemiology is an approach increasingly used in the establishment of associations between exposure to hazardous substances and development of disease, including the possible modulation by genetic susceptibility factors. Environmental chemicals and contaminants from anthropogenic pollution of air, water and soil, but also originating specifically in occupational contexts, are potential sources of risk of development of disease. Also, diet presents an important role in this process, with some well characterized associations existing between nutrition and some types of cancer. Genotoxicity biomarkers allow the detection of early effects that result from the interaction between the individual and the environment; they are therefore important tools in cancer epidemiology and are extensively used in human biomonitoring studies. This work intends to give an overview of the potential for genotoxic effects assessment, specifically with the cytokinesis blocked micronucleus assay and comet assay in environmental and occupational scenarios, including diet. The plasticity of these techniques allows their inclusion in human biomonitoring studies, adding important information with the ultimate aim of disease prevention, in particular cancer, and so it is important that they be included as genotoxicity assays in molecular epidemiology.

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Section: Comet Assaymentioning

confidence: 99%

Untitled

2017

AIMS Genetics

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“…Perhaps most importantly, these tests can help establish correlations between exposure and subsequent illness in individuals -which is often the key observation to prove whether or not a link exists between both (Sexton et al, 2004;Angerer et al, 2007). The advantages of human biomonitoring for the individuals being studied include: identification of exposure, identification of environmental mutagens/carcinogens, and determination of the possible range of susceptibility of humans to specific mutagens and carcinogens (Valverde & Rojas, 2009). In summary, nowadays human biomonitoring of dose and biochemical effect has tremendous utility providing an efficient and cost effective means of measuring human exposure to chemical substances providing unequivocal evidence that both exposure and uptake have been taken place (Sexton et al, 2004;Angerer et al, 2007).…”

Section: Introduction 1human Biomonitoringmentioning

confidence: 99%

International Symposium on Occupational Safety and Hygiene: Proceedings Book of the SHO2016

2013

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A Sociedade Portuguesa de Segurança e Higiene Ocupacionais realiza, em 23 e 24 de março de 2016, a 11ª edição do Colóquio Internacional de Segurança e Higiene Ocupacionais - SHO 2016. Tal como nos dez últimos anos, o evento tem lugar no Auditório Nobre da Escola de Engenharia da Universidade do Minho, em Guimarães. A edição de 2016 abrange as temáticas da Ergonomia e do Ambiente Físico, dos Riscos Químicos e Biológicos, da Segurança contra Incêndio e da Gestão da Prevenção, que são objeto de sessões plenárias, decorrendo ainda várias sessões paralelas de comunicações livres e apresentação de posters.

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“…It consists of standardized protocols aimed at the periodic detection of early (preferably reversible) biological signs, ie biomarkers which are indicative, if compared with adequate reference values, of an actual or potential condition of exposure, effect or susceptibility, possibly resulting in health damage or disease. Biomarkers are usually more specific and sensitive than most clinical tests, and therefore may be more effective for assessing a causal relationship between health impairment and chemical exposure when a change is first detected in exposed workers [4,10,34].…”

Section: Biological Monitoring In Occupational Contextmentioning

confidence: 99%

Human Biomonitoring – An overview on biomarkers and their application in Occupational and Environmental Health

Ladeira¹,

Viegas²

2016

Biomonitoring

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Human biomonitoring (HBM) is a scientificallydeveloped approach for assessing human exposures to natural and synthetic compounds from environment, occupation, and lifestyle. It relies on the measurement of particular substances or biological breakdown products, known as metabolites, in human tissues and/or fluids, and also includes the study of their effects and the possible influence of individual susceptibility as response modulators. HBM is a growing area of knowledge used for exposure and risk assessment in environmental and occupational health, and its importance has been increasing as a result of advancements in the ability to measure greater numbers of chemicals in the human body and tissues. In order to achieve this purpose, HBM focuses on the use of biomarkers as measurable indicators of early changes in biological systems. However, because data interpretation requires caution, it is strongly recommended that the interpretation of HBM results be combined with air monitoring data or pharmacokinetic modelling in order to better understand exposure sources and the metabolism of chemicals.

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The Comet Assay in Human Biomonitoring

Cited by 3 publications

References 152 publications

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International Symposium on Occupational Safety and Hygiene: Proceedings Book of the SHO2016

Human Biomonitoring – An overview on biomarkers and their application in Occupational and Environmental Health

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