In this research work, thermal properties of plain woven fabrics generated from regenerated bamboo and cotton fiber blended yarns were investigated. Seven mixtures of fiber (100% bamboo, 100% cotton, 10:90 bamboo: cotton, 20:80 bamboo: cotton, 30:70 bamboo: cotton, 40:60 bamboo: cotton and 50:50 bamboo: cotton) were developed to create 60 Tex ring spun yarn. The warp yarns were used as 100% regenerated bamboo and the bamboo: cotton blends were used alternatively in weft to produce plain woven fabrics. The plain structured woven fabrics show eminent thermal comfort properties with the blending of regenerated bamboo fibers. The air permeability of 100% regenerated bamboo fiber was recorded higher than the compared blends; the increased key factor contents of bamboo changed the air properties of the fabric. Furthermore, plain woven fabric of bamboo/cotton (50/50) has shown greater thermal conductivity and heat retention properties. The work reported in this paper is ensuring highpoints of thermal comfort properties of regenerated bamboo (100%) and cotton (100%) with plain woven structured fabrics, and potentially, the fabrics can be used for winter suiting apparel products.