The comparative analysis of infection pattern and oocyst output in Eimeria tenella, E. maxima and E. acervulina in young broiler chicken

You, Ming

doi:10.14202/vetworld.2014.542-547

Cited by 19 publications

(18 citation statements)

References 16 publications

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“…Similar findings are recorded by earlier workers Zulpo et al (2007) [25] who reported that E. tenella oocyst per gram of faces on 7DPI is higher than that of E. acervulina. However, the present study contradicts with earlier reports of you (2014) [23] who reported that E. acervulina infected chickens showed higher oocyst shedding than E. tenella and E. maxima. The highest number of oocyst per gram of faces in E. tenella infected group in the present study could be due to the peak multiplication and more rapid excystation of E. tenella sporozoites when compared to E. acervulina and mixed Eimeria species.…”

Section: Discussioncontrasting

confidence: 99%

“…One bird from each group was sacrificed and examined to confirm the absence of any parasitic stage of Eimeria species and other pathological lesions at 21 th day of age. The treatment groups of chickens (G-I, G-II, G-III) were infected artificially infected with sporulated Eimeria oocyts at the age of three weeks as described by You (2014) [23] . They were infected orally with infective dose of 2x10 4 sporulated oocysts of E. tenella, E. acervulina and field isolated mixed Eimeria oocysts.…”

Section: Experimental Design and Inoculation Of Sporulated Oocystsmentioning

confidence: 99%

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Studies on Coccidia in Experimental Infection with Eimeria spp in Rose-Cobb Broiler Chicken

Melkamu¹,

Chanie²,

Asrat³

2017

Jour. Anim. Rese.

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The experimental completely randomized design was conducted on poultry coccidiosis of Rose-Cobb breed of one hundred broiler chicken from November 2015 to April 2016 in the Faculty of Veterinary Medicine at the premises of Tewodrose Campus, University of Gondar, Ethiopia. The objectives of this study were carried out to identify, diagnose and to assess potential effect of Eimeria spp in broiler chicken. The experimental animals (n=100) were randomly allocated into four equal groups, group one and mixed Eimeria spp (G-III) was 2x10 4 sporulated Eimeria oocyst inoculated orally at three weeks of age in broiler chicken and subsequent alterations in different haematological constituents were evaluated at interval of 5, 7 and 9 day of post inoculation The study involved clinical sign and weight gain, morphology identification of Eimeria spp and oocyst count of Eimeria egg per gram of faces. The Eimeria species identified from the natural infected chickens were E. tenella, E. acervulina, E. maxima and E. necatrix. The clinical signs manifested mainly were loss of weight, severe anaemia and bloody watery diarrhoea. Morbidity in all three infected groups were 100%. E. tenella was more pathogenic than E. acervulina and mixed Eimeria spp. There was no statistical difference in the average body weight obtained at 7DPI between the experimental infected and control group. Oocyst counts per gram of faeces in pure (E. tenella and E. acervulina) and mixed Eimeria spp. infection showed marked increase from the 5 to 8DPI. Pure single infection of E. acervulina and E. tenella showed highest oocyst excretion on 6 DPI (62,395 oocyst/g of faeces) and 7 DPI (86,535 oocyst/g of faeces) respectively. Whereas mixed Eimeria spp. infection reached a maximum oocyst count of 51, 270 oocyst/gm of faeces on 7DPI.

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Section: Discussioncontrasting

confidence: 99%

Section: Experimental Design and Inoculation Of Sporulated Oocystsmentioning

confidence: 99%

Studies on Coccidia in Experimental Infection with Eimeria spp in Rose-Cobb Broiler Chicken

Melkamu¹,

Chanie²,

Asrat³

2017

Jour. Anim. Rese.

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“…Having confirmed the nutritional and health-promoting activity of carotenoid-rich corn in a farm environment, we carried out an additional feeding trial incorporating a challenge with 3 9 10 4 sporulated E. tenella oocysts (Houghton strain). This is representative of the typical infective dose, which has been estimated as 1 9 10 4 oocysts, although this is affected by the concurrent use of drugs and vaccines (Dr agan et al, 2014;You, 2014).…”

Section: Protection Of Poultry Against Eimeria Tenellamentioning

confidence: 99%

Carotenoid‐enriched transgenic corn delivers bioavailable carotenoids to poultry and protects them against coccidiosis

Nogareda

Moreno

Angulo

et al. 2015

Plant Biotechnology Journal

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SummaryCarotenoids are health-promoting organic molecules that act as antioxidants and essential nutrients. We show that chickens raised on a diet enriched with an engineered corn variety containing very high levels of four key carotenoids (b-carotene, lycopene, zeaxanthin and lutein) are healthy and accumulate more bioavailable carotenoids in peripheral tissues, muscle, skin and fat, and more retinol in the liver, than birds fed on standard corn diets (including commercial corn supplemented with colour additives). Birds were challenged with the protozoan parasite Eimeria tenella and those on the high-carotenoid diet grew normally, suffered only mild disease symptoms (diarrhoea, footpad dermatitis and digital ulcers) and had lower faecal oocyst counts than birds on the control diet. Our results demonstrate that carotenoid-rich corn maintains poultry health and increases the nutritional value of poultry products without the use of feed additives.

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“…Sampling points were determined by previous studies, which have shown that cytokine response begins to peak at around d3‐4 pi, at the predilection site for E maxima , and subsides by d10 pi, with some sets of cytokines being upregulated at early stages of the infection (ie, pro‐inflammatory) and others later during the infection period (ie, anti‐inflammatory) which coincide with the initiation of anorexia . Parasite genome copy number peaks between d5‐6, and oocyst production is maximized between d6 and d7 pi . The infection protocol consisted of three treatments (uninfected, 2500, 7500) replicated four times with six birds per replicate (n = 72) for each broiler genome.…”

Section: Methodsmentioning

confidence: 99%

“…26 Parasite genome copy number peaks between d5-6, 2,27 and oocyst production is maximized between d6 and d7 pi. 28 The infection protocol consisted of three treatments (uninfected, 2500, 7500) replicated four times with six birds per replicate (n = 72) for each broiler genome. On day 6 and day 13 pi, a randomly selected bird from each pen were culled and necropsied.…”

Section: Broiler Chickensmentioning

confidence: 99%

Differential immune response to Eimeria maxima infection in fast‐ and slow‐growing broiler genotypes

Giles

Sakkas

Belkhiri

et al. 2019

Parasite Immunology

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Very little has been reported comparing resistance to coccidiosis in fast or slow growing broilers, the latter of which are becoming more prevalent in the broiler industry. We examined mRNA expression in the intestines of fast and slow growing broilers following Eimeria infection. We show that by day 13 post‐infection (d pi) with 2500 or 7000 oocysts of Eimeria maxima, slower‐growing (Ranger Classic) broilers significantly (P < 0.01) upregulated expression of proinflammatory cyclooxygenase genes (LTB4DH, PTSG1 and PTSG2) above that detected in fast growing (Ross 308) broilers. Expression of CD8α mRNA was downregulated in Ross 308 at day 6d pi with either 2500 or 7000 oocysts of E maxima (P < 0.05), compared to uninfected controls, but was not differentially expressed in Ranger Classic. CD4 genes were not differentially expressed in either chicken line infected with either infectious oocyst dose at d6 pi, compared to uninfected controls. However, at d13 pi, CD4 expression was significantly upregulated in both chicken lines infected with either infectious oocyst dose, compared to uninfected controls (P < 0.05) but this was significantly greater in Ranger Classic broilers compared to Ross 308 (P < 0.05). At d13 pi, expression of CD3 chains (required for T lymphocyte activation) was significantly increased in Ranger Classic compared to Ross 308, infected with either oocyst dose (P < 0.05‐0.01). Expression of IL‐2 and IL‐15 mRNA, required for T lymphocyte proliferation was also significantly upregulated, or maintained longer, in Ranger Classic broilers compared to Ross 308. These differences in immune response to E maxima corresponded with a reduction in E maxima genome detected in the intestines of Ranger Classic compared to Ross 308.

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The comparative analysis of infection pattern and oocyst output in Eimeria tenella, E. maxima and E. acervulina in young broiler chicken

Cited by 19 publications

References 16 publications

Studies on Coccidia in Experimental Infection with Eimeria spp in Rose-Cobb Broiler Chicken

Studies on Coccidia in Experimental Infection with Eimeria spp in Rose-Cobb Broiler Chicken

Carotenoid‐enriched transgenic corn delivers bioavailable carotenoids to poultry and protects them against coccidiosis

Differential immune response to Eimeria maxima infection in fast‐ and slow‐growing broiler genotypes

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