The Comparative Biology of Classical Swine Fever

Harkness, J.W.; Roeder, P. L.

doi:10.1007/978-1-4613-2083-8_12

Cited by 5 publications

(2 citation statements)

References 141 publications

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“…HCV -the causative virus of hog cholera (classical swine fever), one of the most dangerous infectious diseases of swine -is considered the most important of the pestiviruses, due to the economic losses caused to the pig industry by this disease. The ability of pestiviruses to cross species barriers (7), their close relationship, and the frequent occurrence of atypical forms of infection caused by HCV strains with mild or low virulence (12) complicate the diagnosis and make control of the disease difficult.…”

Section: Introductionmentioning

confidence: 99%

Reverse transcription combined with polymerase chain reaction as a detection method for pestiviral infections

Stadejek

Pejsak²,

Kwinkowski³

et al. 1995

Rev. Sci. Tech. OIE

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An assay based on reverse transcription coupled with the polymerase chain reaction (RT-PCR) was used for the detection of hog cholera virus (HCV) and bovine virus diarrhoea virus (BVDV) in cell culture. In this study, a precipitate of the supernatants derived from cell cultures infected with HCV and BVDV was used in RT reactions, in place of extracted viral RNA. Both RT and PCR were performed using recombinant Thermus thermophilus (rTth) DNA polymerase. The specificity of the RT-PCR products was confirmed by hybridisation with a digoxygenin-labelled DNA probe. The results not only show that the stage of RNA isolation can be bypassed, but also illustrate an easy and efficient means of obtaining templates suitable for identification and characterisation of HCV and BVDV in tissue culture by RT-PCR.

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Section: Introductionmentioning

confidence: 99%

Reverse transcription combined with polymerase chain reaction as a detection method for pestiviral infections

Stadejek

Pejsak²,

Kwinkowski³

et al. 1995

Rev. Sci. Tech. OIE

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“…derived from fetal calf serum or produced from cell cultures fortuitously infected with BVDV (45,43). More information on the serologic evidence of virus distribution is given in a recent review on the relationship between ruminant pestiviruses and HCV infections in pigs (14).…”

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confidence: 99%

Ruminant pestivirus infection in pigs

Ließ¹,

Moennig²

1990

Rev. Sci. Tech. OIE

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Ruminant pestivirus infections of pigs have a worldwide distribution. The prevalence is varied and depends mainly on (i) contact with cattle, (ii) age of pigs and (iii) degree of homology of virus strains used for serology, with field strains of bovine virus diarrhoea virus (BVDV) infecting pigs. Emphasis should be laid on sources of BVDV other than cattle, e.g. contaminated vaccines and fetal calf serum. The need for differentiation of pestiviruses (hog cholera, bovine virus diarrhoea and Border disease viruses) is highlighted by the fact that clinical disease syndromes, e.g. growth retardation and wasting, are reminiscent of hog cholera. Monoclonal antibodies are available which differentiate between hog cholera virus (HCV) and ruminant pestiviruses, presumably BVDV. An up-to-date account of the antigenic relationship between pestiviruses is included in the review. Analysis of the in vitro host range of these viruses is considered to be important and may explain infections of pigs with pestiviruses other than HCV. Recent results have shown the existence of "specialists" amongst BVDV strains for bovine cells, and a few isolates also performed well in cultures of the PK15 cell line. In contrast, multipotent BVDV strains presumably have additional attachment sites for ovine and porcine cells. Identification of receptors on ovine and porcine cells could contribute to a clear distinction between BVDV and HCV infections of pigs. Immediate control measures for BVDV infections of pigs are not required. However, such infections may interfere with serologic surveys and surveillance on a herd basis and, therefore, impair eradication programmes and efforts to maintain the status in countries declared free of hog cholera.

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The impact of pestivirus on an artificial breeding program for cattle

Kirkland

Hart²,

Moyle

et al. 1990

Aust Veterinary J

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After the introduction of pestivirus into a herd undergoing an embryo transfer and artificial insemination program, substantial post-weaning calf losses occurred. The predominant clinical feature was severe respiratory disease, in contrast to the commonly recognised mucosal disease. Thirty-one of 76 calves were affected, with a case fatality rate of 58%. All calves which were persistently infected with pestivirus died during the study period. There was a significant association in the surviving calves between the occurrence of recent pestivirus infection and respiratory disease. The losses on this property clearly indicate the need to routinely screen animals in an artificial breeding program for freedom from pestivirus infection.

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The Comparative Biology of Classical Swine Fever

Cited by 5 publications

References 141 publications

Reverse transcription combined with polymerase chain reaction as a detection method for pestiviral infections

Reverse transcription combined with polymerase chain reaction as a detection method for pestiviral infections

Ruminant pestivirus infection in pigs

The impact of pestivirus on an artificial breeding program for cattle

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