The comparision ofCoprinus cinereusperoxidase enzyme and TiO₂catalyst for phenol removal

Abstract

This article investigates phenol removal from an aqueous solution by using enzymatic and photocatalytic methods and the efficiency of these methods has been compared. In enzymatic and photocatalytic methods, Coprinus cinereus, peroxidase enzyme and commercial TiO(2) powders (Degussa P-25) in aqueous suspension were used, respectively, in ambient temperature. The effects of different operating parameters such as duration of process, catalyst dosage or enzyme concentration, pH of the solution, initial phenol concentration and H(2)O(2) concentration on both processes were examined. In enzymatic method, efficiency of degradation reached 100% within 5 min, while in the photocatalytic method, the efficiency of degradation reached approximately 70% within 60 min. In photocatalytic method, there is an optimum concentration for catalyst dosage (near 2.0 g/L) to gain 80% efficiency, while in the enzymatic method, increasing the amount of enzyme could lead to an increase in the efficiency up to 100%. Moreover, the optimum pH in enzymatic and photocatalytic methods stood at 8.0 and 7.0, respectively. In both methods, the addition of different amounts of H(2)O(2) increased the degradation efficiency to 100%.

Optimization of an Activity Assay of Coprinus Cinereus Peroxidase

“…CIP has higher thermostability than HRP 9,10 . It has been used successfully to remove phenolic compounds from wastewater [11][12][13][14][15][16] , to degrade benzene homologs and derivates 17,18 , to decolourate dyes [19][20][21] , and to produce functional polyaromatics [22][23][24][25][26] . CIP can also be used as cleaners 27 and biosensors 28 .…”

Investigation of Extraction of Phenol from Wastewater UsingN,N-Didodecyl-1-dodecanamine (Tridodecylamine) in Benzene

“…Thus, there is an urgent need to find an effective method for the treatment of wastewater containing phenol. Several methods have been developed to remove phenol from wastewater, including microbial degradation, chemical oxidation, photocatalytic degradation, ultrasonic degradation, enzymatic polymerization, membrane separation, solvent extraction, adsorption, and steam-distillation. − In India, the desirable limit of phenolic compounds in the drinking water as phenol is 0.001 ppm, the limit in the effluents of land water is 1 ppm, and the limit in the sewer discharge is 5 ppm . The phenolic compounds are also used in the production of many chemicals such as dyes, adhesives, germicides, and chemical intermediates and thus are considered as valuable chemicals.…”