The Comparison and Analysis of Three Extraction Methods for Polysaccharides in Purslane

Abstract

In this study, three extraction methods of polysaccharides in Purslane were compared and analyzed, and the best method was ascertained. Purslane polysaccharides were extracted using water, ultrasonic and microwave. By the results of single factor experiments and orthogonal experiments, the optimal extracting conditions were selected and then the best extraction method was determined. In order to calculate the extraction yield of polysaccharides, its content were measured by the phenol-sulfuric acid method using glucose as the standard, and then the efficiency of three extraction methods was evaluated. The results showed that the extraction yield of ultrasonic was 35.42% under the optimal conditions of 60°C, 60 min, 1:20 and 200w. The extraction yield of microwave method was 32.12% under the optimal conditions of 3 min, 1:25 and 500w. The extraction yield of water method was 31.22% under the optimal conditions of 80°C, 2 h, 1:30 and 3 times. It could be concluded that ultrasonic method was the best one in these three methods.

Influence of different extraction techniques on recovery, purity, antioxidant activities, and microstructure of flaxseed gum

“…When the extraction time extended, the plant cells ruptured, increasing the release rate of protein in the extraction liquid and lower solvent permeability (Fabre et al., 2015; Thirugnanasambandham et al., 2015; J. Wang et al., 2010). Comparatively, Gu and Pan (2014) reported the highest extraction yield (32.12%) after 3 min for purslane polysaccharide. Although MAE offers a feasible method to rapidly increase the temperature of the sample solution or medium to carry out successive processes, though it did not present a significant influence on the overall extraction yield of the gum.…”

“…HWE is a traditional method, easy to control without any special apparatus or conditions (Gu & Pan, 2014). In current study, FSG extraction was accomplished at various extraction temperatures (40, 60, 80, and 100 °C) regarding yield, carbohydrate, and protein content (Figure 2b and 3b).…”

“…Additionally, higher temperature led to splitting of seed coat, resulting in extraction of more protein that is mainly present in the endosperm (Barbary, Al‐Sohaimy, El‐Saadani, & Zeitoun, 2009; Fabre et al., 2015; Kaushik et al., 2017). Gu and Pan (2014) reported that HWE method does not destroy the polysaccharide molecules under controlled conditions. Obtained results are in the same manner with Kaushik et al.…”

“…MAE was performed using a microwave oven (Samsung, Model PG‐3200, Korea) according to Gu and Pan (2014) at different extraction time durations, that is, 3, 6, 9, 12, and 15 min at a power of 900 W with a frequency of 2,450 MHz. Further process was same as mentioned above in HWE.…”

Prebiotic efficacy of coconut kernel cake’s soluble crude polysaccharides on growth rates and acidifying property of probiotic lactic acid bacteria in vitro

“…Water extraction is a mild and stable procedure, which is easy to control and does not destroy the polysaccharide molecules. However, it is associated with long extraction time and high temperature [29]. The higher yield in alkaline pH is relevant to the structure of polysaccharide and isoelectric point of protein.…”