The comparison of polymorphism among Avena species revealed by retrotransposon-based DNA markers and soluble carbohydrates in seeds

Abstract

Here, we compared the polymorphism among 13 Avena species revealed by the iPBS markers and soluble carbohydrate profiles in seeds. The application of seven iPBS markers generated 83 bands, out of which 20.5% were polymorphic. No species-specific bands were scored. Shannon’s information index (I) and expected heterozygosity (He) revealed low genetic diversity, with the highest values observed for A. nuda (I = 0.099; He = 0.068). UPGMA clustering of studied Avena accessions and PCoA results showed that the polyploidy level is the main grouping criterion. High-resolution gas chromatography revealed that the studied Avena accessions share the same composition of soluble carbohydrates, but significant differences in the content of total (5.30–22.38 mg g−1 of dry weight) and particular sugars among studied samples were observed. Sucrose appeared as the most abundant sugar (mean 61.52% of total soluble carbohydrates), followed by raffinose family oligosaccharides (31.23%), myo-inositol and its galactosides (6.16%), and monosaccharides (1.09%). The pattern of interspecific variation in soluble carbohydrates, showed by PCA, was convergent to that revealed by iPBS markers. Thus, both methods appeared as a source of valuable data useful in the characterization of Avena resources or in the discussion on the evolution of this genus.

Phenotypic and genetic characterization of an Avena sativa L. germplasm collection of diverse origin: implications for food-oat breeding in Chile

“…0 0 0 -0 . 0 6 8 ) u s i n g retrotransposon primer binding sites-iPBS (Androsiuk et al, 2023). Thus, the mostly moderate genetic diversity observed here, which is coincident with most other studies from around the world, shows a limitation for oat breeding, indicating the need to increase the diversity using different strategies to ensure genetic gain in the long term.…”

“…Having said that, similar genetic diversity (He) compared to the present study was obtained in a group of 16 exotic oat genotypes from Europe and Pakistan (0.12-0.53) using five RAPD primers with 23 loci amplified ( Jan et al., 2020 ); in 18 oat cultivars from Russia, Norway, Netherlands, and Sweden (0.33-0.75) based on avenin-like alleles ( Lyubimova et al., 2020 ); in 64 oat cultivars from Europe (0.5-0.63) using seven SSRs ( Nersting et al., 2006 ); in 288 oat genotypes from diverse origin (0.096-0.50) using 2143 SNPs ( Wang et al., 2023 ); and in a 260 naked, husked and black oats collection (0.48-0.61) using 15 SSRs ( Leišová-Svobodová et al., 2019 ). However, low genetic diversity was found in 23 oat cultivars from Poland (0.20) using the dominant markers ISSR, RAPDs and AFLPs ( Boczkowska et al., 2016 ), in 177 red and white oats from southern Spain (0.29) using 31 SSRs ( Montilla-Bascón et al., 2013 ), in 72 Polish oats (0.15-0.30) using 36 ISSRs ( Koroluk et al., 2022 ), and in 60 accessions representing 13 Avena species (0.000-0.068) using retrotransposon primer binding sites-iPBS ( Androsiuk et al., 2023 ). Thus, the mostly moderate genetic diversity observed here, which is coincident with most other studies from around the world, shows a limitation for oat breeding, indicating the need to increase the diversity using different strategies to ensure genetic gain in the long term.…”