The Comparison Of The Effects Of Platelet Rich Plasma And Demineralized Bone Matrix On Critical Bone Defects: An Experimental Study On Rats

Turhan, Egemen; Akça, Mustafa Kemal; Bayar, Ahmet; Songür, Murat; Keser, Selçuk; Doral, Mahmut Nedim

doi:10.5505/tjtes.2016.68249

Cited by 4 publications

(8 citation statements)

References 34 publications

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“…The other goal of the study was to nd a way to increase bone healing in addition to suitable scaffold. PRP were proven to increase bone healing in the literature previously [20]. In our study, histologically, PRP combination with omentum had higher organized ossi cation and bone mineralization than the omentum only group.…”

Section: Discussionsupporting

confidence: 65%

Effect of a Decellularized Omentum Scaffold With Combination of Mesenchymal Stem Cells and Platelet Rich Plasma on Healing of Critical-sized Bone Defect

Emet

Özdemir

Çetinkaya

et al. 2021

Preprint

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Background: High-cost and the long time needed for the treatment of critical-sized bone defects is still major clinical concern in orthopedic surgery, therefore new research continues to identify more cost and time-effective methods. In this study, we aimed to investigate the effect of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical- sized bone defects. Materlials and methods: Wistar albino rats (n=30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. To increase healing, no scaffold, decellularized omentum, omentum with with PRP and omentum mesenchymal stem cells was used in group 1 (control group) , 2, 3 and 4 respectively. In addition omentum with a combination of mesenchymal stem cells and PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among groups. Results: After use of decellularized omentum scaffold, vitality of new cells maintained, new bone formation occurred. When compared to control group, radiological healing was statistically significantly better in omentum and omentum with PRP-treated groups (p=0.047). Furthermore histological healing was better in the omentum and omentum with PRP-treated groups than both control and omentum with mesenchymal stem cell-treated groups according to Salkeld Scoring System (p=0.001).Conclusion: The use of decellularized omentum scaffold is suitable in the healing of critical bone defects.

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Section: Discussionsupporting

confidence: 65%

Effect of a Decellularized Omentum Scaffold With Combination of Mesenchymal Stem Cells and Platelet Rich Plasma on Healing of Critical-sized Bone Defect

Emet

Özdemir

Çetinkaya

et al. 2021

Preprint

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“…These findings are in agreement with the only previous study that investigated the DBM efficacy in a canine tibial nonunion model, showing that a DBM‐based treatment stimulates defect healing (Tiedeman et al, ). The efficacy of DBM for nonunion treatment is also indirectly supported by the studies showing that DBM promotes bone defect healing in large segmental defects (Einhorn, Lane, Burstein, Kopman, & Vigorita, ; Turhan et al, ; Xing et al, ; Xu et al, ). DBM consists of an osteoconductive matrix that could recruit osteoprogenitor cells.…”

Section: Discussionmentioning

confidence: 92%

Nonunion fracture healing: Evaluation of effectiveness of demineralized bone matrix and mesenchymal stem cells in a novel sheep bone nonunion model

Dozza

Salamanna

Baleani

et al. 2018

J Tissue Eng Regen Med

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Nonunion treatment has a high rate of success, although recalcitrant nonunion may determine the need for amputation. Therefore, new treatment options are continuously investigated in order to further reduce the risk of nonunion recurrence. This study aimed to (a) develop a new large animal model for bone atrophic nonunion and (b) compare the efficacy of demineralized bone matrix (DBM) and DBM in combination with mesenchymal stem cells (MSC) in the new nonunion model. The new model consists of a noncritical, full-thickness segmental defect created in the sheep tibia, stabilized by an intramedullary nail, and involves the creation of a locally impaired blood supply achieved through periosteum excision and electrocauterization of the stump ends. Six weeks after defect creation, lack of hard tissue callus and established nonunion was observed in all operated tibiae both by radiographic and clinical evaluation. Nonunion was treated with allogeneic DBM or autologous MSC cultivated on DBM particles (DBM + MSC) for 1 day before implantation. Twelve weeks after treatment, radiographic, microtomographic, histologic, and histomorphometric analysis showed the formation of bone callus in DBM group, whereas the fracture healing appeared at an early stage in DBM + MSC group. Torsional strength and stiffness of the DBM group appeared higher than those of DBM + MSC group, although the differences were not statistically significant. In conclusion, a new sheep bone nonunion model resembling the complexity of the clinical condition was developed. DBM is an effective option for nonunion treatment, whereas MSC do not improve the healing process when cultivated on DBM particles before implantation.

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“…The other goal of the study was to find a way to increase bone healing in addition to a suitable scaffold. PRP was proven to increase bone healing in the literature previously [20].…”

Section: Discussionmentioning

confidence: 99%

Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model

et al. 2021

Self Cite

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The high costs and extensive time needed for the treatment of critical-sized bone defects are still major clinical concerns in orthopedic surgery; therefore, researchers continue to look for more cost and time-effective methods. This study aims to investigate the effects of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical-sized bone defects. Wistar albino rats (n = 30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. No scaffold, decellularized omentum, omentum with PRP and omentum + mesenchymal stem cells was used in group 1 (control group), 2, 3 and 4, respectively. In addition, omentum with a combination of mesenchymal stem cells +PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among the groups. After the use of a decellularized omentum scaffold, vitality of new cells was maintained, and new bone formation occurred. When compared to the control group, radiological healing was significantly better (p = 0.047) in the omentum and omentum + PRP-treated groups. Furthermore, histological healing was better in the omentum and omentum + PRP-treated groups than the control group (p = 0.001). The use of a decellularized omentum scaffold is suitable in the healing of critical bone defects.

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The Comparison Of The Effects Of Platelet Rich Plasma And Demineralized Bone Matrix On Critical Bone Defects: An Experimental Study On Rats

Cited by 4 publications

References 34 publications

Effect of a Decellularized Omentum Scaffold With Combination of Mesenchymal Stem Cells and Platelet Rich Plasma on Healing of Critical-sized Bone Defect

Effect of a Decellularized Omentum Scaffold With Combination of Mesenchymal Stem Cells and Platelet Rich Plasma on Healing of Critical-sized Bone Defect

Nonunion fracture healing: Evaluation of effectiveness of demineralized bone matrix and mesenchymal stem cells in a novel sheep bone nonunion model

Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model

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