The comparison study of bioactivity between composites containing synthetic non-substituted and carbonate-substituted hydroxyapatite

Borkowski, Leszek; Sroka-Bartnicka, Anna; Drączkowski, Piotr; Ptak, Agnieszka; Zięba, Emil; Ślósarczyk, A.; Ginalska, Grażyna

doi:10.1016/j.msec.2016.01.056

Cited by 24 publications

(13 citation statements)

References 44 publications

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“…The apatite-forming assay was adopted to evaluate the acellular bioactivity of the various modified coatings. According to a previous method, 12 the coated substrates were soaked at 37°C in simulated body fluid (SBF) under an agitation of 100 rpm for 14 days, and the medium was replaced every 3 days for fresh SBF exposure. After a prescribed time, SBF on the samples was removed and immediately dried in a desiccator.…”

Section: Apatite-forming Ability Assaymentioning

confidence: 99%

“…7,8 According to the substitution of carbonate groups for hydroxyl groups or phosphate ions, carbonated apatite can be categorized into A-type or the more diffused B-type, and the latter has been verified to cause a decrease in crystallinity and hence an increase in solubility. 9 Thus far, carbonated hydroxyapatite (CHA) has mostly been applied as ceramic bone substitutes; [10][11][12] meanwhile, it has also gradually become employed as coatings on implant substrates 13,14 and drug delivery systems. 15 CHA-related investigations have largely reached a consensus on its excellent biocompatibility and bio-resorption properties, yet conflicting results regarding the biological effects on osteoblasts, including adhesion, proliferation and osteogenic differentiation, are still being reported.…”

Section: Introductionmentioning

confidence: 99%

“…15 CHA-related investigations have largely reached a consensus on its excellent biocompatibility and bio-resorption properties, yet conflicting results regarding the biological effects on osteoblasts, including adhesion, proliferation and osteogenic differentiation, are still being reported. [11][12][13]15,16 The genuine biological functions of CHA biomaterials compared with those of HA products still remain ambiguous and relatively undesirable, mostly because the content of carbonate incorporated into HA was either undetermined or limited to a low concentration range according to the available literature. As a matter of fact, HA with a higher carbonate content mimicking early biomineralization could contribute to better cell behaviors, as implied by a study involving a wide spectrum of carbonate concentrations from 0 to 16.1 wt%.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of highly carbonated hydroxyapatite bioceramic implant coatings with hierarchical micro-/nanorod topography optimized for osseointegration

Li¹,

Yu²,

Zhang³

et al. 2018

IJN

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BackgroundOptimal osseointegration has been recognized as a pivotal factor in determining the long-term success of biomedical implants.Materials and methodsIn the current study, highly carbonated hydroxyapatite (CHA) with carbonate contents of 8, 12 and 16 wt% and pure hydroxyapatite (HA) were fabricated via a novel hydrothermal method and deposited on the titanium substrates to generate corresponding CHA bioceramic coatings (designated as C8, C12 and C16, respectively) and HA bioceramic coatings (designated as C0).ResultsC8, C12 and C16 were endowed with nanoscale, hierarchical hybrid micro-/nanoscale and microscale surface topographies with rod-like superstructures, respectively. Compared with C0, the micro-/nanotextured CHA bioceramic coatings (C8, C12 and C16) possessed excellent surface bioactivity and biocompatibility, as well as better wettability, which mediated improved protein adsorption, giving rise to simultaneous enhancement of a biological cascade of events of rat bone-marrow-derived mesenchymal stem cells including cell adhesion, proliferation, osteogenic differentiation and, notably, the production of the pro-angiogenic growth factor, vascular endothelial growth factor-A. In particular, C12 with biomimetic hierarchical hybrid micro-/nanorod topography exhibited superior fractal property and predominant performance of protein adsorption, cell adhesion, proliferation and osteogenesis concomitant with angiogenesis.ConclusionAll these results suggest that the 12 wt% CHA bioceramic coating with synergistic modification of surface chemistry and topography has great prospect for future use as implant coating to achieve optimum osseointegration for orthopedic and dental applications.

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Section: Apatite-forming Ability Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of highly carbonated hydroxyapatite bioceramic implant coatings with hierarchical micro-/nanorod topography optimized for osseointegration

Li¹,

Yu²,

Zhang³

et al. 2018

IJN

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“…HA signal shift indicates that amorphous apatite was found alongside the one derived from composite, as shown by the peak position in the third column of Figure 5 . Mineral, phosphate phase is distributed evenly as a thin layer, 4–13 µm depth, as reported in one of our previous works [ 32 ]. Thus, comparing to strong signal from concentrated granules of HA, phosphate signal originating from new HA layer appears in areas of lower intensity scale.…”

Section: Resultsmentioning

confidence: 55%

Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface

Kalisz

Przekora

Kazimierczak

et al. 2021

IJMS

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Raman spectroscopic imaging and mapping were applied to characterise three-compound ceramic composite biomaterial consisting of chitosan, β-1,3-d-glucan (curdlan) and hydroxyapatite (HA) developed as a bone tissue engineering product (TEP). In this rapidly advancing domain of medical science, the urge for quick, reliable and specific method for products evaluation and tissue–implant interaction, in this case bone formation process, is constantly present. Two types of stem cells, adipose-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMDSCs), were cultured on composite surface. Raman spectroscopic imaging provided advantageous information on molecular differences and spatial distribution of compounds within and between the cell-seeded and untreated samples at a microscopic level. With the use of this, it was possible to confirm composite biocompatibility and bioactivity in vitro. Deposition of HA and changes in its crystallinity along with protein adsorption proved new bone tissue formation in both mesenchymal stem cell samples, where the cells proliferated, differentiated and produced biomineralised extracellular matrix (ECM). The usefulness of spectroscopic Raman imaging was confirmed in tissue engineering in terms of both the organic and inorganic components considering composite–cells interaction.

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“…Recently, Raman spectroscopy (widely used in chemistry) has also found application in the assessment of bone quality. This technique has become a valuable tool in bone implant testing [ 23 ] and bone tissue characterization [ 24 , 25 ]. It allows understanding how changes in bone composition and structure influence tissue-level mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

New approach in evaluation of ceramic-polymer composite bioactivity and biocompatibility

et al. 2017

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Regeneration of bone defects was promoted by a novel β-glucan/carbonate hydroxyapatite composite and characterized by Raman spectroscopy, microCT and electron microscopy. The elastic biomaterial with an apatite-forming ability was developed for bone tissue engineering and implanted into the critical-size defects of rabbits’ tibiae. The bone repair process was analyzed on non-decalcified bone/implant sections during a 6-month regeneration period. Using spectroscopic methods, we were able to determine the presence of amides, lipids and assign the areas of newly formed bone tissue. Raman spectroscopy was also used to assess the chemical changes in the composite before and after the implantation process. SEM analyses showed the mineralization degree in the defect area and that the gap size decreased significantly. Microscopic images revealed that the implant debris were interconnected to the poorly mineralized inner side of a new bone tissue. Our study demonstrated that the composite may serve as a biocompatible background for collagen ingrowth and exhibits the advantages of applying Raman spectroscopy, SEM and microCT in studying these samples.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-017-0518-0) contains supplementary material, which is available to authorized users.

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The comparison study of bioactivity between composites containing synthetic non-substituted and carbonate-substituted hydroxyapatite

Cited by 24 publications

References 44 publications

Evaluation of highly carbonated hydroxyapatite bioceramic implant coatings with hierarchical micro-/nanorod topography optimized for osseointegration

Evaluation of highly carbonated hydroxyapatite bioceramic implant coatings with hierarchical micro-/nanorod topography optimized for osseointegration

Application of Raman Spectroscopic Imaging to Assess the Structural Changes at Cell-Scaffold Interface

New approach in evaluation of ceramic-polymer composite bioactivity and biocompatibility

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