The complete alk sequences of Rhodococcus erythropolis from Lake Baikal

Likhoshvay, Alexander; Lomakina, A. V.; Grachev, M. A.

doi:10.1186/2193-1801-3-621

Cited by 10 publications

(3 citation statements)

References 20 publications

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“…n-alkanes account for up to 88 % of the volume in natural oil and petroleum products and can serve as an energy source for microorganisms capable of decomposing them (Van Bei len et al, 2003;Dedov et al, 2017). The detection of alkanmonooxygenase group genes was previously carried out for bacterial communities isolated from petroleum products, and the activity of strains against the degradation of various hydrocarbons, including n-alkanes, was shown (Likhoshvay et al, 2014;Lomakina et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Activity of alkanmonooxygenase <i>alk</i>B gene in strains of hydrocarbon-oxidizing bacteria isolated from petroleum products

2022

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Alkanmonooxygenase enzymes AlkB and Cyp153 are responsible for the aerobic degradation of n-alkanes of petroleum and petroleum products. To prove the usage of n-alkanes from oil and petroleum products by hydrocarbon- oxidizing bacteria isolated from aviation kerosene TS-1 and automobile gasoline AI-95, the detection of the key genes alkB, Alk1, Alk2, Alk3 and Cyp153 encoding alkanmonooxygenases AlkB and Cyp153 (responsible for the oxidation of hydrocarbons with a certain chain length) was carried out. It was found that bacterial strains isolated from TS-1 jet fuel, except Deinococcus sp. Bi7, had at least one of the studied n-alkane degradation genes. The strains Sphingobacterium multivorum Bi2; Alcaligenes faecalis Bi3; Rhodococcus sp. Bi4; Sphingobacterium sp. Bi5; Rhodococcus erythropolis Bi6 contained the alkB gene. In the strains of hydrocarbon-oxidizing bacteria isolated from gasoline AI- 95, this alkanmonooxygenase gene was not detected. Using the real-time PCR method, the activity of the alkB gene in all bacterial strains isolated from petroleum products was analyzed and the number of its copies was determined. By real-time PCR using a primer with a different sequence of nucleotides to detect the alkB gene, its activity was established in all bacterial strains isolated from gasoline AI-95; besides, the strain Paenibacillus agaridevorans Bi11 was assigned to the group with a high level of its activity (1290 copies/ml). According to the assessment of the growth of isolated hydrocarbon-oxidizing bacteria on a solid Evans mineral medium with the addition of the model mixture of hydrocarbons, the strains were divided into three groups. The distributions of strains of hydrocarbon-oxidizing bacteria in the groups based on the activity of the alkB gene and groups formed based on the growth ability and use of the model mixture of hydrocarbons and petroleum products were found to be consistent. The results obtained indicate that we need to use a complex of molecular and physiological methods for a comprehensive analysis of the distribution of the studied genes in bacteria and to assess their activity in the strains of hydrocarbon-oxidizing bacteria capable of biodegradation of petroleum hydrocarbons.

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Section: Resultsmentioning

confidence: 99%

Activity of alkanmonooxygenase <i>alk</i>B gene in strains of hydrocarbon-oxidizing bacteria isolated from petroleum products

2022

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“…The consumption of hydrophobic hydrocarbon substrates by R. erythropolis is accompanied by the synthesis of surface-active glycolipid compounds. It has been found that its genome contains alk -genes (alkanehydroxylases) responsible for the degradation of numerous n -alkanes [39].…”

Section: Methodsmentioning

confidence: 99%

Protatranes, effective growth biostimulants of hydrocarbon-oxidizing bacteria from Lake Baikal, Russia

Pavlova

Адамович

Novikova

et al. 2019

Biotechnology Reports

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Highlights We have synthesized a wide range of tris-(2-hydroxyethyl)ammonium arylchalcogenylacetates, “protatranes”. “Protatranes” are growth stimulators of hydrocarbon-oxidizing Rhodococcus erythropolis bacteria isolated from Lake Baikal. The effect of “protatranes” on growth of Pseudomonas fluorescens is less obvious. The effect of stimulants on Rhodococcus erythropolis is likely due to the structure of protatranes and the cell wall of bacteria.

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“…Bakteri pendegradasi n-alkana di antaranya adalah Cryptococcus neuformans dan Pseudomonas aeruginosa dari tanah sekitar kilang minyak (Chan, Kuo, Lin, & Mou, 1990), Pseudomonas aeruginosa WatG dan Serratia marcescens HokM yang terdapat pada mata air panas yang terkontaminasi minyak bumi (Wongsa et al 2004), Mycobacterium sp. CH1 yang diisolasi dari sedimen air tawar (Churchill, Harper, & Churchil, 1999), Rhodococcus serytropolis PR4 dari aspal di dekat Tanjung gutes Gorevol (Likhoshvay, Lomakina, & Grachev, 2014), Marinobacter sp. CAB dari stasiun pengeboran minyak di Vietnam Selatan (Huu, Denner, Ha, Wanner, & Lotter, 1999), dan Alcanivorax dieselolei B-5 dari air laut pesisir dan sedimen laut dalam (Liu et al 2011).…”

Section: Pendahuluanunclassified

OPTIMASI PERTUMBUHAN BAKTERI LAUTSalinicola peritrichatus LBF-1-0025 DALAM SENYAWA ALKANA

Lingga

Thontowi²,

Yetti³

et al. 2017

JPBKP

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ABSTRAKPentana, dekana, pentadekana, dan parafin merupakan jenis kelompok senyawa alkana yang tidak dapat larut dalam air dan sulit terdegradasi. Sifat ini yang menjadikan senyawa alkana dapat mencemari lingkungan. Penelitian ini bertujuan untuk mengetahui konsentrasi optimum senyawa alkana bagi pertumbuhan isolat LBF-1-0025 dan mengidentifikasinya secara molekuler berdasarkan gen 16S rDNA. Dari skrining awal diketahui bahwa isolat LBF-1-0025 memiliki potensi tinggi dalam mendegradasi senyawa pentana, dekana, pentadekana, dan parafin. Optimasi pertumbuhan isolat LBF-1-0025 pada beberapa senyawa alkana dilakukan dengan menumbuhkannya dalam medium Artificial Sea Water (ASW ) yang mengandung beberapa konsentrasi senyawa pentana, dekana, pentadekana, dan paraffin. Isolat LBF-1-0025 mampu tumbuh secara optimal pada pentana 150 ppm, dekana 200 ppm, pentadekana 150 ppm, dan parafin 200 ppm setelah 3 hari inkubasi. Hasil analisis sekuen gen 16S rDNA, bakteri LBF-1-0025 memiliki kemiripan sebesar 97% dengan Salinicola peritrichatus DY22.

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The complete alk sequences of Rhodococcus erythropolis from Lake Baikal

Cited by 10 publications

References 20 publications

Activity of alkanmonooxygenase <i>alk</i>B gene in strains of hydrocarbon-oxidizing bacteria isolated from petroleum products

Activity of alkanmonooxygenase <i>alk</i>B gene in strains of hydrocarbon-oxidizing bacteria isolated from petroleum products

Protatranes, effective growth biostimulants of hydrocarbon-oxidizing bacteria from Lake Baikal, Russia

OPTIMASI PERTUMBUHAN BAKTERI LAUTSalinicola peritrichatus LBF-1-0025 DALAM SENYAWA ALKANA

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