The complete chloroplast genome of Cucumis anguria var. anguria (Cucurbitaceae) and its phylogenetic implication

Abstract

2020) The complete chloroplast genome of Cucumisanguriavar.anguria (Cucurbitaceae) and its phylogenetic implication, Mitochondrial DNA Part B, 5:1, 654-655, ABSTRACTThe genus Cucumis contains 52 species, including two economically significant crops, cucumber and melon, as well as other important species. Cucumis anguria var. anguria is a wild relative of C. melon, native to Africa. Cucumis anguria is rich in vitamins and minerals in gherkin fruits and carries broadspectrum resistance to multiplex biotic and abiotic stress, such as powdery mildew, fusarium wilt, and meloidogyn incognita. Cucumis anguria provides a valuable gene pool for crop improvement of Cucumis crops. In this study, the complete chloroplast (cp) genome sequence of C. anguria was determined using next-generation sequencing. The entire cp genome was determined to be 156,577 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 85,971 and 18,100 bp, respectively, which were separated by a pair of 26,253 bp inverted repeat (IR) regions. The genome contained 134 genes, including 88 protein-coding genes, 37 tRNA genes, 8 rRNA genes and 1 pseudogene infA. The overall GC content of the genome is 37.0%. A phylogenetic tree reconstructed by 48 chloroplast genomes reveals that C. anguria is a separate branch in Cucumis. ARTICLE HISTORY

The complete chloroplast genome sequences of nine melon varieties (Cucumis melo L.): lights into comparative analysis and phylogenetic relationships

“…In this study, we assembled and compared the chloroplast genomes of nine different melon varieties. The genome size, gene content, gene sequence and GC content were all similar across the melon varieties indicating that the chloroplast genome of melon is highly conserved ( Cheng et al, 2020 ). The chloroplast genomes from the nine different melon species exhibit a typical quadripartite molecular structure including four important regions: a large single-copy (LSC) region, a small single-copy (SSC) region and a pair of inverted repeats (IRs) region ( Powell et al, 1995 ; Jansen et al, 2005 ).…”

“…The majority of angiosperms have haploid chloroplast genomes, which are a valuable resource for research into the phylogeny and evolution of plants due to their uni-parental inheritance ( Wang H. X. et al, 2020 ). The phylogenetic relationship among the different genera in the Cucurbitaceae has been explored based on the chloroplast genome ( Wang L. et al, 2020 ; Cheng et al, 2020 ). However, few studies apply the chloroplast genome to the in-depth study of distinguishing between C. melo ssp .…”