The complete chloroplast genome of aquilegia rockii, an endemic herb plant in Western China

Abstract

The complete chloroplast genome sequence of Aquilegia rockii was assembled and characterized as a resource for future genetic studies. With a total length of 162,123 bp, the chloroplast genome comprised of a large single-copy (LSC) region of 91,337 bp, a small single-copy (SSC) region of 17,376 bp, and 2 inverted repeat (IR) regions of 26,705 bp. A total of 117 unique genes, including 81 protein-coding genes, 4 ribosomal RNA genes, and 32 tRNA genes. The maximum likelihood phylogenetic analysis based on 18 cp genomes indicated that A. rockii is sister to the clade formed by Semiaquilegia adoxoides and Urophysa rockii.

The complete chloroplast genome of Aquilegia barnebyi, a basal eudicot species

“…The data was deposited in GenBank with the accession number MN882557. As other taxa in the family of Ranunculaceae (Yu et al 2019), the cp genome of A. barnebyi displayed the typical quadripartite structure, including a pair of inverted repeat regions (IR with 26,671 bp) divided by two single-copy regions (LSC 91,250 bp and SSC 17,359 bp). The overall GC content of the cp genome was 38.98%.…”

“… 2009 ; Yu et al. 2019 ). The mapped chloroplast reads were assembled using Geneious v7.1.7 (Biomatters, New Zealand) with the A. rockii cp genome as reference.…”

“…The chloroplast reads were filtered from whole genome Illumina sequencing data of A. barnebyi which was downloaded from NCBI Short Read Archive (SRR7965809) (Filiault et al 2018). We mapped all the sequencing reads to the A. rockii cp genome using bowtie2 (v2.3.4.3) (Langmead et al 2009;Yu et al 2019). The mapped chloroplast reads were assembled using Geneious v7.1.7 (Biomatters, New Zealand) with the A. rockii cp genome as reference.…”