The complete chloroplast genome of sweet tea (<i>Lithocarpus polystachyus</i>)

Lithocarpus litseifolius (Hance) Chun 1837 is an evergreen tree of Fagaceae, which can be used as sweet tea, natural sweetener, and precious medicinal material. The complete chloroplast genome of L. litseifolius was sequenced and its phylogenetic relationship was analyzed in this study. The chloroplast genome of L. litseifolius has a circular structure with a length of 161,322 bp, and it contains a pair of inverted repeat regions (IRs 25,897 bp), a large single copy (LSC 90,551 bp), and a small single copy (SSC 18,977 bp). There were 131 genes identified, including 37 tRNA, 8 rRNA, and 86 mRNA genes. Phylogenetic analysis of 23 species of Fagaceae indicated that Lithocarpus is monophyletic with strong bootstrap, and L. litseifolius is genetically closely related to Lithocarpus polystachyus.

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“…This phylogenetic result was similar to those of Li et al. ( 2019 ), Ma et al. ( 2021 ), and Wu et al.…”

Section: Discussionsupporting

confidence: 92%

“… 2021 ), Lithocarpus polystachyus MK914534.1 (Li et al. 2019 ), Lithocarpus konishii ON422319.1 (unpublished), Lithocarpus litseifollus OM048987.1 (unpublished), Lithocarpus balansae KP299291.1 (Li et al. 2018 ), Lithocarpus longinux OK181903.1 (Wu et al.…”

Section: Methodsmentioning

confidence: 99%

The complete chloroplast genome sequence ofLithocarpus litseifolius(Hance) Chun 1837 (Fagaceae) and phylogenetic analysis

Qiu

Xiao

Tian

et al. 2023

Mitochondrial DNA Part B

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“…Lithocarpus polystachyus Rehd. (LP) belongs to the Lithocarpus arbor of the Fagaceae family and is widely distributed in some Asian countries, including China, Thailand, and India [ 1 ]. In southern China, tender leaves and flower spikes with sweet tastes have a long history of usage for tea making among ethnic minorities.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition

Wei

Tuo²,

Zhang

et al. 2020

Journal of Analytical Methods in Chemistry

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The purpose of this work is to establish a new method using high-performance liquid chromatography-diode array detection (HPLC-DAD) with chemometrics analysis to determine the content of catechin, isoquercetin, astragalin, phloridzin, trilobatin, and phloretin for one flavanol and five flavonoids, filter out the key compounds, and evaluate the quality of 26 batches of tender leaves and flower spikes of Lithocarpus polystachyus Rehd. (LP) from ten areas in China. The result showed that the HPLC-DAD method had excellent performance for accurate quantification analysis. S3 (tender leaf from Lushan, Sichuan) had the highest contents for six measured chemicals with trilobatin content of up to 27.82% in dry weight. S22 (flower spike from Liangping, Chongqing) had the highest content of phloridzin (up to 7.28%). All samples were divided into three types based on spatial distribution using principal component analysis. The result showed that the tender leaves and flower spikes from the same areas had many similar properties, and there were significant differences between the samples from different regions. Furthermore, phloridzin and trilobatin were identified as chemical markers for quality evaluation of two parts with different tender leaves and flower spikes of LP from geographical areas by orthogonal partial least squares discrimination analysis. These results will be helpful to establish an effective and comprehensive evaluation system of the development and utilization of LP resources.

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“…The chloroplast genome (cp-genome) sequences and annotation of 8 species of Lithocarpus (L. balansae, L. dealbatus [1], L. glaber, L. hancei [15], L. konishii [6], L. litseifolius [2], L. longinux [7], and L. polystachyus [5]), three Quercus (Q. baronii [16], Q. bawanglingensis [17], and Q. variabilis [18]), and two Corylus (C. fargesii [19] and C. heterophylla) were downloaded from the NCBI. Statistics of gene content were extracted from annotation files (.gff3) using 'gt gff3' and "gt stat" GenomeTools (genometools-genometools v1.5.10) [20].…”

Section: Chloroplast Genome Contentmentioning

confidence: 99%

“…Not only play an essential role in forests as food resources [4], but several species have also been used as medicinal plants to prevent diabetes [2]. L. litseifolius and L. polystachyus are known as sweet tea [2,5]. Over-exploitation, illegal trading, habitat destruction and fragmentation were reportedly responsible for the population decline [6].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of Lithocarpus chloroplast genomes reveals candidate DNA barcoding loci

Herliana,

Chairunisa,

Wahyuni

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Lithocarpus or stone oaks (Fagaceae) are ecologically and economically important trees in tropical and subtropical Asia as animal food sources, valuable timber trees, and natural bioactive components. Nonetheless, it was often challenging to identify Lithocarpus at the species level. Using a short sequence of specific regions in the genome, DNA barcoding offers a fast and cost-effective way to catalogue biodiversity and protect endangered species from illegal trades. However, universal barcodes were reported as not always applicable to all plant species. Thus, specific barcodes have been introduced based on the analysis of whole chloroplast genomes. This study aimed to identify specific barcodes for species-level differentiation within the Lithocarpus genus. Publicly available chloroplast genomes of eight Lithocarpus, three Quercus (Fagaceae) as ingroups and two Corylus (Betulaceae) as outgroups were compared. The number of protein-coding genes ranged between 80 – 87. Of 80 orthogroups identified, fifty-nine groups contained single-copy orthologous genes (SOGs). Only two genes showed high sequence variation: matK and ndhF. We recommend future investigation of these two genes as candidate barcoding loci to identify intraspecific variation at species and population levels. These barcodes will accelerate species identification of Lithocarpus from fresh, herbaria or degraded samples.

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The complete chloroplast genome of sweet tea (Lithocarpus polystachyus)

Cited by 7 publications

References 9 publications

The complete chloroplast genome sequence ofLithocarpus litseifolius(Hance) Chun 1837 (Fagaceae) and phylogenetic analysis

The complete chloroplast genome sequence ofLithocarpus litseifolius(Hance) Chun 1837 (Fagaceae) and phylogenetic analysis

Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition

Comparative analysis of Lithocarpus chloroplast genomes reveals candidate DNA barcoding loci

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