“…2 ). Protocol 1, which utilizes fresh leaves ground in liquid nitrogen, was used for extracting DNA from V. trifolia [ 10 ], Citrus micrantha [ 11 ], V. parviflora [ 12 ], Mentha ˣ villosa [ 13 ], V. bicolor [ 14 ], Senna alata [ 15 ] and Allium chinense [ 16 ]. The assembled chloroplast genomes are used as plant super-barcodes to elucidate the evolutionary relationship of closely related species [ 14 ].…”