The complete genome of the antifungal bacterium Pseudomonas sp. strain MS82

Ma, Lin; Qu, Shaoxuan; Lin, Jing; Jia, Jiayuan; Baird, Sonya M.; Jiang, Ning; Li, Huiping; Hou, Lijuan; Lu, Shi‐En

doi:10.1007/s41348-019-00205-z

Cited by 9 publications

(5 citation statements)

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“…R45Sugar cane rhizosphere soil sample in Piracicaba-SP, BrazilNDNEHH00000000[51] Pseudomonas sp. MS586Cotton field, USANDCP014205.2 P. fluorescens MS82Soybean rhizosphere, Mississippi, USANDCP028826.1[71] Sequence Housekeeping genes accession rpoB rpoD 16S rDNA gyrB P. chlororaphis subsp. aureofaciens LMG 1245 T AJ717426.1FN554453AY509898FN554172 P. koreensis LMG 21318 T FN554737FN554476AF468452FN554194 P. aeruginosa DSM 50071 T CP012001CP012001CP012001CP012001 …”

Section: Table A1mentioning

confidence: 99%

Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

et al. 2019

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Pseudomonas species are metabolically robust, with capacity to produce secondary metabolites including cyclic lipopeptides (CLPs). Herein we conducted a chemical analysis of a crude CLP extract from the cocoyam rhizosphere-derived biocontrol strain Pseudomonas sp. COW3. We performed in silico analyses on its whole genome, and conducted in vitro antagonistic assay using the strain and purified CLPs. Via LC-MS and NMR, we elucidated the structures of four novel members of the bananamide group, named bananamides D-G. Besides variability in fatty acid length, bananamides D-G differ from previously described bananamides A-C and MD-0066 by the presence of a serine and aspartic acid at position 6 and 2, respectively. In addition, bananamide G has valine instead of isoleucine at position 8. Kendrick mass defect (KMD) allowed the assignment of molecular formulae to bananamides D and E. We unraveled a non-ribosomal peptide synthetase cluster banA, banB and banC which encodes the novel bananamide derivatives. Furthermore, COW3 displayed antagonistic activity and mycophagy against Pythium myriotylum, while it mainly showed mycophagy on Pyricularia oryzae. Purified bananamides D-G inhibited the growth of P. myriotylum and P. oryzae and caused hyphal distortion. Our study shows the complementarity of chemical analyses and genome mining in the discovery and elucidation of novel CLPs. In addition, structurally diverse bananamides differ in their antimicrobial activity.

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Section: Table A1mentioning

confidence: 99%

Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

et al. 2019

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“…Strain MS586 T has shown remarkable antifungal activities against a broad range of plant fungal pathogens (Jia and Lu, unpublished). Similarly, our study has demonstrated that strain MS82 possesses antifungal activities against the mushroom fungal pathogen Mycogone perniciosa , but not the mushroom fungus (Ma et al, 2019). Furthermore, it has been reported that PafR gene confers resistance to the mushroom pathogenic fungus (Ma et al, 2017).…”

Section: Resultsmentioning

confidence: 64%

Pseudomonas glycinae sp. nov. isolated from the soybean rhizosphere

et al. 2020

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Strains MS586T and MS82, which are aerobic, Gram‐negative, rod‐shaped, and polar‐flagellated bacteria, were isolated from the soybean rhizosphere in Mississippi. Taxonomic positions of MS586T and MS82 were determined using a polyphasic approach. 16S rRNA gene sequence analyses of the two strains showed high pairwise sequence similarities (>98%) to some Pseudomonas species. Analysis of the concatenated 16S rRNA, rpoB, rpoD, and gyrB gene sequences indicated that the strains belonging to the Pseudomonas koreensis subgroup (SG) shared the highest similarity with Pseudomonas kribbensis strain 46‐2T. Analyses of average nucleotide identity (ANI), genome‐to‐genome distance, delineated MS586T and MS82 from other species within the genus Pseudomonas. The predominant quinone system of the strain was ubiquinone 9 (Q‐9), and the DNA G+C content was 60.48 mol%. The major fatty acids were C16:0, C17:0 cyclo, and the summed features 3 and 8 consisting of C16:1ω7c/C16:1ω6c and C18:1ω7c/C18:1ω6c, respectively. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. Based on these data, it is proposed that strains MS586T and MS82 represent a novel species within the genus Pseudomonas. The proposed name for the new species is Pseudomonas glycinae, and the type strain is MS586T (accession NRRL B‐65441 = accession LMG 30275).

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“…The control of Trichoderma mainly relies on environmental control methods for prevention. Some safe agents ( Innocenti et al, 2019 ) or biocontrol microorganisms ( Ma et al, 2019 ) can be used to control Trichoderma during the hypha growth stage. However, the agent may have the potential to cause phytotoxicity ( Kwon et al, 2021 ) or residues ( Li et al, 2022 ) during the fruiting body growth stage.…”

Section: Discussionmentioning

confidence: 99%

Identification of a pathogen causing fruiting body rot of Sanghuangporus vaninii

Yuan,

Ma,

Chen

et al. 2023

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Sanghuangporus vaninii is a medicinal macrofungus that is increasingly cultivated in China. During cultivation, it was found that the fruiting body of S. vaninii was susceptible to pathogenic fungi, resulting in significant economic losses to the industry. The symptoms of the disease occur in the initial stage of fruiting body development. The isolate YZB-1 was obtained from the junction of the diseased and healthy areas of the fruiting body. In order to verify the pathogenicity of YZB-1, its purified spore suspension was inoculated into the exposed area nearby the developing fruiting body of S. vaninii. After 10 days, the same disease symptoms appeared in the inoculated area. Morphological identification and molecular analysis of rDNA ITS region confirmed that the isolate YZB-1 was identified as Trichoderma virens. The temperature stability assay revealed that the mycelia of YZB-1 grew the fastest at 25 °C, with growth slowing down gradually as the temperature increased or decreased. Dual-culture tests of T. virens and S. vaninii showed that the inhibition rate of T. virens on S. vaninii mycelium was the highest (79.01 ± 2.79%) at 25 °C, and more green spores were produced at the intersection of T. virens and S. vaninii.

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The complete genome of the antifungal bacterium Pseudomonas sp. strain MS82

Cited by 9 publications

References 44 publications

Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

Pseudomonas sp. COW3 Produces New Bananamide-Type Cyclic Lipopeptides with Antimicrobial Activity against Pythium myriotylum and Pyricularia oryzae

Pseudomonas glycinae sp. nov. isolated from the soybean rhizosphere

Identification of a pathogen causing fruiting body rot of Sanghuangporus vaninii

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