The complete genome sequence of New World jatropha mosaic virus

Abstract

Full-length sequences of a bipartite begomovirus were obtained from a plant of Jatropha multifida in Florida showing symptoms of foliar mosaic, distortion and necrosis. Sequences of four clones each of a DNA-A and DNA-B were obtained, which showed very low sequence diversity among themselves. The clones were infectious when biolistically inoculated to J. multifida, Phaseolus vulgaris and Nicotiana tabacum, but not to J. curcas. The DNA-A sequences had less than 89 % pairwise identity scores with the DNA-A of other begomoviruses. The DNA-A appeared to be a recombinant in that 18 % of the DNA-A (470 nt) had a pairwise identity score of 91.98 % with RhRGMV, indicating that this portion most likely originated from a virus closely related to RhRGMV. The remaining 82 % of the DNA-A had lower identity scores with TbMoLCV (87.84 %) and RhRGMV (87.46 %), which suggests that this part of the component originated from an undescribed virus. There was no evidence for recombination in the DNA-B. Equivalent sequences of the DNA-A had the highest identity score (94.18 %) with a 533-nt sequence obtained from J. multifida from Puerto Rico in 2001 (GenBank accession no. AF058025). Pairwise comparison, recombination and phylogenetic analysis, and biology suggest that these clones are those of jatropha mosaic virus first reported from Puerto Rico. This is the first report of the complete genome sequence of jatropha mosaic virus.

Identification and characterization of a novel geminivirus with a monopartite genome infecting apple trees

“…Only a few years ago, citrus and grapevine were reported to be the first woody plants naturally infected by different geminiviruses (Krenz et al, 2012;Loconsole et al, 2012), followed more recently by J. multifida (Polston et al, 2014). In this work, using NGS and bioinformatics tools, we identified a novel geminivirus in apple trees, thus extending the natural hosts of this important virus family to another economically relevant woody crop.…”

“…This conclusion is further supported by the analyses of phylogenetic relationships between AGV and representative geminiviruses, selected to include (i) the type species of each genera of the family Geminiviridae, (ii) other species representative of subgroups proposed in some genera of this family based on phylogenetic, biological and/or epidemiological features (Fauquet et al, 2008;Varsani et al, 2014), (iii) geminivirus species sharing a certain sequence identity with AGV (Table 1), and (iv) unclassified geminiviruses infecting woody plants (Al Rwahnih et al, 2013;Loconsole et al, 2012;Polston et al, 2014). When a phylogenetic tree was reconstructed using the Rep protein encoded by these viruses, AGV was clustered together with other members of the genus Begomovirus (Fig.…”

“…Geminiviruses are the cause of devastating plant diseases, particularly in the tropics and subtropics (Moffat, 1999;Varma & Malathi, 2003). Although most geminiviruses infect herbaceous hosts, three geminiviruses have been reported in woody hosts, including citrus (associated with citrus chlorotic dwarf disease) (Loconsole et al, 2012), grape (associated with grapevine red blotch and red leaf diseases) (Al Rwahnih et al, 2013;Poojari et al, 2013) and, more recently, Jatropha multifida (associated with foliar mosaic, distortion and necrosis in infected plants) (Polston et al, 2014).…”

Identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease

“…In the last few years, identification of divergent geminiviruses infecting citrus, grapevine and Jatropha multifida (Al Rwahnih et al, 2013;Krenz et al, 2012;Loconsole et al, 2012;Polston et al, 2014;Poojari et al, 2013) has shown that the host range of geminiviruses is not limited to herbaceous plants. In this study we report the identification and characterization of a novel geminivirus infecting mulberry trees, as an addition to the list of woody plants infected by geminiviruses in nature.…”

Characterization of a novel begomovirus associated with yellow mosaic disease of three ornamental species of Jatropha grown in India

“…African cassava mosaic virus (syn. Cassava latent virus) from Kenya (Bock et al, 1981) and Jatropha mosaic virus from Florida (Polston et al, 2014) have also been reported on J. multifida. However, no virus on J. integerrima and J. podagrica has been reported hitherto.…”