The complete genome sequences of four new IncN plasmids from wastewater treatment plant effluent provide new insights into IncN plasmid diversity and evolution

Eikmeyer, Felix Gregor; Hadiati, Atika; Szczepanowski, Rafael; Wibberg, Daniel; Schneiker-Bekel, Susanne; Rogers, Linda; Brown, Celeste; Top, Eva M.; Pühler, Alfred; Schlüter, Andreas

doi:10.1016/j.plasmid.2012.01.011

Cited by 61 publications

(37 citation statements)

References 84 publications

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“…Previous studies have found that tetracycline resistance genes, such as tet(M), tet(W), and tet(Q), are always present in transposons and other MGEs. 41−43 The sulII gene is found to be located on four IncN plasmids in WWTPs effluent, 44 and these IncN plasmids have also been detected in clinical samples, and regarded as having human health relevance. A plasmid metagenome analysis from the final effluent of a WWTP in Germany has revealed 140 clinically relevant ARGs including genes encoding resistance to aminoglycosides, beta-lactams, fluoroquinolones, macrolides, tetracyclines, and other antibiotics.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

Wang

Qiao

et al. 2014

Environ. Sci. Technol.

395

201

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Reclaimed water irrigation (RWI) in urban environments is becoming popular, due to rapid urbanization and water shortage. The continuous release of residual antibiotics and antibiotic resistance genes (ARGs) from reclaimed water could result in the dissemination of ARGs in the downstream environment. This study provides a comprehensive profile of ARGs in park soils exposed to RWI through a high-throughput quantitative PCR approach. 147 ARGs encoding for resistance to a broad-spectrum of antibiotics were detected among all park soil samples. Aminoglycoside and beta-lactam were the two most dominant types of ARGs, and antibiotic deactivation and efflux pump were the two most dominant mechanisms in these RWI samples. The total enrichment of ARGs varied from 99.3-fold to 8655.3-fold compared to respective controls. Six to 60 ARGs were statistically enriched among these RWI samples. Four transposase genes were detected in RWI samples. TnpA-04 was the most enriched transposase gene with an enrichment was up to 2501.3-fold in Urumqi RWI samples compared with control soil samples. Furthermore, significantly positive correlation was found between ARGs and transposase abundances, indicating that transposase might be involved in the propagation of ARGs. This study demonstrated that RWI resulted in the enrichment of ARGs in urban park soils.

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Section: ■ Results and Discussionmentioning

confidence: 99%

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

Wang

Qiao

et al. 2014

Environ. Sci. Technol.

395

201

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“…3) with quite a narrow host range (Table 2), but it is the first IncN plasmid to be completely devoid of antibiotic resistance genes (59,60). It has two accessory regions between fipA and nuc and between kikA and the EcoRII type II restriction modification gene complex.…”

Section: Resultsmentioning

confidence: 99%

“…The IncN plasmid, pDS2 has accessory genes but, surprisingly, none that encode antibiotic resistance. IncN plasmids are considered a health risk because they typically confer a variety of antibiotic resistances (59). As far as we know, pDS2 is the first IncN plasmid that is devoid of antibiotic resistance genes.…”

Section: Fig 5 Unique Plasmid Restriction Fragment Patterns Of Capturmentioning

confidence: 99%

Diverse Broad-Host-Range Plasmids from Freshwater Carry Few Accessory Genes

Brown

Sen

Yano

et al. 2013

Appl Environ Microbiol

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c Broad-host-range self-transferable plasmids are known to facilitate bacterial adaptation by spreading genes between phylogenetically distinct hosts. These plasmids typically have a conserved backbone region and a variable accessory region that encodes host-beneficial traits. We do not know, however, how well plasmids that do not encode accessory functions can survive in nature. The goal of this study was to characterize the backbone and accessory gene content of plasmids that were captured from freshwater sources without selecting for a particular phenotype or cultivating their host. To do this, triparental matings were used such that the only required phenotype was the plasmid's ability to mobilize a nonconjugative plasmid. Based on complete genome sequences of 10 plasmids, only 5 carried identifiable accessory gene regions, and none carried antibiotic resistance genes. The plasmids belong to four known incompatibility groups (IncN, IncP-1, IncU, and IncW) and two potentially new groups. Eight of the plasmids were shown to have a broad host range, being able to transfer into alpha-, beta-, and gammaproteobacteria. Because of the absence of antibiotic resistance genes, we resampled one of the sites and compared the proportion of captured plasmids that conferred antibiotic resistance to their hosts with the proportion of such plasmids captured from the effluent of a local wastewater treatment plant. Few of the captured plasmids from either site encoded antibiotic resistance. A high diversity of plasmids that encode no or unknown accessory functions is thus readily found in freshwater habitats. The question remains how the plasmids persist in these microbial communities.

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“…Comparative analysis (3,6,15) and interpretation of the genome sequence revealed that M. bourgensis MS2…”

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confidence: 99%

Complete Genome Sequence of the Hydrogenotrophic, Methanogenic Archaeon Methanoculleus bourgensis Strain MS2 ^T , Isolated from a Sewage Sludge Digester

et al. 2012

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c Methanoculleus bourgensis, of the order Methanomicrobiales, is a dominant methanogenic archaeon in many biogas-producing reactor systems fed with renewable primary products. It is capable of synthesizing methane via the hydrogenotrophic pathway utilizing hydrogen and carbon dioxide or formate as the substrates. Here we report the complete and finished genome sequence of M. bourgensis strain MS2T , isolated from a sewage sludge digester.

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The complete genome sequences of four new IncN plasmids from wastewater treatment plant effluent provide new insights into IncN plasmid diversity and evolution

Cited by 61 publications

References 84 publications

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

Diverse Broad-Host-Range Plasmids from Freshwater Carry Few Accessory Genes

Complete Genome Sequence of the Hydrogenotrophic, Methanogenic Archaeon Methanoculleus bourgensis Strain MS2 ^T , Isolated from a Sewage Sludge Digester

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The complete genome sequences of four new IncN plasmids from wastewater treatment plant effluent provide new insights into IncN plasmid diversity and evolution

Cited by 61 publications

References 84 publications

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

Diverse Broad-Host-Range Plasmids from Freshwater Carry Few Accessory Genes

Complete Genome Sequence of the Hydrogenotrophic, Methanogenic Archaeon Methanoculleus bourgensis Strain MS2 T , Isolated from a Sewage Sludge Digester

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Complete Genome Sequence of the Hydrogenotrophic, Methanogenic Archaeon Methanoculleus bourgensis Strain MS2 ^T , Isolated from a Sewage Sludge Digester