The complete mitochondrial genome of the Basidiomycete edible fungus <i>Hypsizygus marmoreus</i>

Wu, Ying‐Ying; Shang, Junjun; Li, Yan; Chun, Zhou; Hou, Di; Li, Jiali; Tan, Qian; Bao, Dapeng; Yang, Runqiang

doi:10.1080/23802359.2018.1532343

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…The H. marmoreus strain, Finc-W-247, was collected from Shanghai Finc Bio-tech Inc., Shanghai, China. H. marmoreus was sequenced with Pacbio and Illumina [ 43 ]. RNAiso Plus, PrimeScript TM RT reagent Kit, and SYBR ® Premix Ex Taq II were purchased from TaKaRa (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…Amino acid sequences of FIPs, listed in Supplementary Table S2, were downloaded from NCBI (https://www.ncbi.nlm.nih.gov (accessed on 9 October 2022)). To identify immunomodulatory protein homologous genes in H. marmoreus, c13717 amino acid sequence was used as a bait [44], and a local BLAST search was performed to search for immunomodulatory protein homologous genes from the sequenced H. marmoreus genome [43].…”

Section: Bioinformatics Analysismentioning

confidence: 99%

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Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Wang

et al. 2023

Molecules

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A novel fungal immunomodulatory protein (FIP), identified as FIP-hma, was discovered in the genome of an edible mushroom Hypsizygus marmoreus. Bioinformatics analysis suggested FIP-hma contained the cerato-platanin (CP) conserved domain and was categorized into Cerato-type FIP. In phylogenetic analysis, FIP-hma was clustered into a new branch of the FIP family, displaying large system divergence from most of the other FIPs. The higher gene expression of FIP-hma was observed during the vegetative growth stages than that during the reproductive growth stages. In addition, the cDNA sequence of FIP-hma was cloned and successfully expressed in Escherichia coli (E. coli) BL21(DE3). The recombinant protein of FIP-hma (rFIP-hma) was neatly purified and isolated by Ni-NTA and SUMO-Protease. The iNOS, IL-6, IL-1β, and TNF-α levels of RAW 264.7 macrophages were upregulated by rFIP-hma, indicating its activation of an immune response by regulating central cytokines. No cytotoxic effects were observed in an MTT test. The findings of this work discovered a novel immunoregulatory protein from H. marmoreus, provided a systematic bioinformatic profile, suggested an effective approach for its heterologous recombinant production, and reported its potent immunoregulatory activity in macrophages. This study sheds light on the physiological function research of FIPs and their further industrial utilization.

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Section: Methodsmentioning

confidence: 99%

Section: Bioinformatics Analysismentioning

confidence: 99%

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Wang

et al. 2023

Molecules

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“… 2016 , 2017 ; Wu et al. 2018 ). Finally, a total of 6.64 Gb raw data generated from the platform Hiseq4000.…”

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confidence: 99%

The complete mitochondrial genome of the edible mushroom Grifola frondosa

Song

Wan

Shang

et al. 2022

Mitochondrial DNA Part B

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The culinary-medicinal mushroom Grifola frondosa is widely cultivated in East Asia. In this study, the complete mitochondrial genome of G. frondosa was determined using Illumina sequencing. The circular molecule was 197,486 bp in length with a content of 25.01% GC, which was one of the largest mitochondrial genomes in the order Polyporales. A total of 39 known genes encoding 13 common mitochondrial genes, 24 tRNA genes, 1 ribosomal protein s3 gene ( rps3 ), and 1 DNA polymerase gene ( dpo ) were predicted in this genome. The phylogenetic analysis showed that G. frondosa clustered together with Sparassis crispa , Laetiporus sulphureus , Wolfiporia cocos , and Taiwanofungus camphoratus . The complete mitochondrial genome reported here may provide new insight into genetic information and evolution for further studies.

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GC-IMS-Based Volatile Characteristic Analysis of Hypsizygus marmoreus Dried by Different Methods

Lai,

Li,

Wei

et al. 2024

Foods

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Gas chromatography–ion mobility spectroscopy (GC-IMS) was used to analyze the volatile components in dried Hypsizygus marmoreus of different drying methods, including hot air drying (HAD), heat pump drying (HPD), heated freeze-drying (HFD), and unheated freeze-drying (UFD). A total of 116 signal peaks corresponding to 96 volatile compounds were identified, including 25 esters, 24 aldehydes, 23 alcohols, 13 ketones, 10 heterocyclic compounds, 8 carboxylic acids, 7 terpenes, 3 sulfur-containing compounds, 2 nitrogen-containing compounds, and 1 aromatic hydrocarbon. The total content of volatile compounds in H. marmoreus dried by the four methods, from highest to lowest, was as follows: HAD, HPD, HFD, and UFD. The main volatile compounds included carboxylic acids, alcohols, esters, and aldehydes. Comparing the peak intensities of volatile compounds in dried H. marmoreus using different drying methods, it was found that the synthesis of esters, aldehydes, and terpenes increased under hot drying methods such as HAD and HPD, while the synthesis of compounds containing sulfur and nitrogen increased under freeze-drying methods such as HFD and UFD. Nine common key characteristic flavor compounds of dried H. marmoreus were screened using relative odor activity values (ROAV > 1), including ethyl 3-methylbutanoate, acetic acid, 2-methylbutanal, propanal, methyl 2-propenyl sulfate, trimethylamine, 3-octanone, acetaldehide, and thiophene. In the odor description of volatile compounds with ROAV > 0.1, it was found that important flavor components such as trimethylamine, 3-octanone, (E)-2-octenal, and dimethyl disulfide are related to the aroma of seafood. Their ROAV order is HFD > UFD > HPD > HAD, indicating that H. marmoreus using the HFD method have the strongest seafood flavor. The research findings provide theoretical guidance for selecting drying methods and refining the processing of H. marmoreus.

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The complete mitochondrial genome of the Basidiomycete edible fungus Hypsizygus marmoreus

Cited by 3 publications

References 19 publications

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

The complete mitochondrial genome of the edible mushroom Grifola frondosa

GC-IMS-Based Volatile Characteristic Analysis of Hypsizygus marmoreus Dried by Different Methods

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