The complete mitochondrial genome of Xizicus (Haploxizicus) maculatus revealed by Next-Generation Sequencing and phylogenetic implication (Orthoptera, Meconematinae)

Abstract

Xizicus Gorochov, 1993, the quiet-calling katydid, is a diverse genus with 68 species in world, which includes more than 45 species in China, has undergone numerous taxonomic revisions with contradicting conclusions. In this study the complete mitochondrial genome of Xizicus (Haploxizicus) maculatus collected from Hainan for the first time was sequenced using the Next-Generation Sequencing (NGS) technology. The length of whole mitogenome is 16,358 bp and contains the typical gene arrangement, base composition, and codon usage found in other related species. The overall base composition of the mitochondrial genome is 37.0 % A, 32.2 % T, 20.2 % C, and 10.6 % G. All 13 protein-coding genes (PCGs) began with typical ATN initiation codon. Nine of the 13 PCGs have a complete termination codon, but the remaining four genes (COI, COIII, ND5, and ND4) terminate with an incomplete T. Phylogenetic analyses are carried out based on the concatenated dataset of 13 PCGs and two rRNAs of Tettigoniidae species available in GenBank. Both Bayesian inference and Maximum Likelihood analyses recovered each subfamily as a monophyletic group. Regardless of the position of Lipotactinae, the relationships among the subfamilies of Tettigoniidae were as follows: ((((Tettigoniinae, Bradyporinae) Meconematinae) Conocephalinae) Hexacentrinae). The topological structure of the phylogeny trees showed that the Xizicus (Haploxizicus) maculatus is closer to Xizicus (Xizicus) fascipes than Xizicus (Eoxizicus) howardi.

Tracking outbreak populations of the pepper weevil Anthonomus eugenii (Coleoptera; Curculionidae) using complete mitochondrial genomes

“…eugenii . Our results further support the application of complete mitochondrial genomes to infer phylogenetic relations between insect specimens as was previously shown for stoneflies [35] and bush crickets [36].…”

The complete mitochondrial genome sequence of Scolopendra mutilans L. Koch, 1878 (Scolopendromorpha, Scolopendridae), with a comparative analysis of other centipede genomes

“…In order to identify interspersed repeats or intra-genomic duplications of large fragments throughout the mitogenomes, we performed BLASTn searches of the mitogenome against itself using an E-value of 1e-10. Tandem repeats within the mitogenome were detected by MicroSAtellite (MISA) (Shaoli et al 2018;Thiel et al 2003), with the following thresholds: ten, six, five, five, five, and five repeat units for mononucleotide, di-nucleotide, tri-nucleotide, tetranucleotide, penta-nucleotide, and hexanucleotide SSRs. Forward (direct), reverse, complemented, and palindromic (reverse complemented) repeats were identified using the REPuter software (Kurtz et al 2001) with default settings.…”

Mitochondrial genome characterization of Gryllodes sigillatus (Orthoptera: Gryllidae) and its phylogenetic implications

“…G. sigillatus was first described in 1869 and then classified into different genera many times (Otte 2006). Insect mitogenome sequences have been extensively used to infer the evolution and phylogeny of metazoan at both deep and shallow taxonomic levels due to its fast mutation rate, conservation among conspecifics and lack of recombination (Cameron 2014;Li et al 2019;Shaoli et al 2018). Here, we reported the complete mitogenome sequence of G. sigillatus, the first in the Genus Gryllodes, which would contribute to evolution and phylogeny of Ensifera.…”

“… 2019 ; Shaoli et al. 2018 ). Here, we reported the complete mitogenome sequence of G. sigillatus , the first in the Genus Gryllodes , which would contribute to evolution and phylogeny of Ensifera.…”