The complete mitochondrial genome of the Pale-legged Leaf-Warbler Phylloscopus tenellipes (Aves: Phylloscopidae) from Maor Mountain, China

Abstract

The Pale-legged Leaf-Warbler ( Phylloscopus tenellipes ) (Swinhoe, 1860) is an olive-brown warbler that is extensively dispersed in Asia. We sequenced the whole mitochondrial genome of a Pale-legged Leaf-Warbler collected on Maor Mountain, Heilongjiang Province, China. The mitochondrial genome is 16,972 bp and contains 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and one control region (CR). The CR is 1098 bp. The nucleotide sequence is composed of 29.11% A, 22.98% T, 14.64% G, and 33.27% C. Phylogenetic research revealed that P. tenellipes is closely related to Phylloscopus borealoides .

Mitochondrial genome characteristics of six Phylloscopus species and their phylogenetic implication

“…In clade I, the closer relationship of the sister group between P. borealoides and P. tenellipes (BS = 100; PP = 1.00) was consistent with previous studies based on mitochondrial genes and nuclear segments ( Johansson et al, 2007 ; Alström et al, 2018 ; Sun, Liu & Lu, 2020 ; Yu et al, 2022 ). The sister group between P. borealis and P. examinandus (BS = 100; PP = 1.00) agreed with previous studies ( Alström et al, 2018 ).…”

“…The sister group between P. borealis and P. examinandus (BS = 100; PP = 1.00) agreed with previous studies ( Alström et al, 2018 ). Within clade II, a closer relationship between P. inornatus and P. proregulus (BS = 100; PP = 1.00) was observed, consistent with previous analyses utilizing concatenated mitochondrial gene datasets ( Jiao et al, 2018 ; Yu et al, 2022 ). Furthermore, the genus Seicercus was nested within Phylloscopus ( Fig.…”

“…Based on the phylogeny of mitochondrial and nuclear datasets, Alström et al (2018) supported the synonymizing of Seicercus with Phylloscopus , leading to the synonymization of Seicercus and a monogeneric Phylloscopidae. The phylogenetic relationship and divergences of Phylloscopus have also been examined using multiple molecular markers such as mitochondrial genomes (mitogenomes) ( Yu et al, 2022 ), mitochondrial genes combined with nuclear segments ( Alström et al, 2018 ), and genome-wide data ( Zhang et al, 2021 ). Other research has also been carried out on leaf warblers, in relation to topics such as their biogeographic history ( Johansson et al, 2007 ), bioacoustic differentiation ( Calviño Cancela, Piña & Martín-Herrero, 2022 ), and migratory behavior ( Adams et al, 2022 ).…”

“…By sampling ∼300 representatives of Passeriformes mitogenomes, Mackiewicz et al (2019) analyzed four types of gene rearrangements, including a duplicated CR with adjacent genes, indicating that the duplication was the ancestral state and was maintained in early diverged lineages. Several complete mitogenomes of Phylloscopus are available in the GenBank database, such as those for Phylloscopus tenellipes ( Sun, Liu & Lu, 2020 ; Yu et al, 2022 ) and Phylloscopus proregulus ( Jiao et al, 2018 ), whose data have been used for the analysis of phylogenetic relationships of this genus and even the whole Passeriformes order.…”