The complete mitochondrial genome of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) isolated from Leonurus japonicus in Korea

Abstract

We have determined the mitochondrial genome of A. gossypii isolated from Leonurus japonicus in Korea. The circular mitogenome of A. gossypii is 16,044 bp, including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNAs, and a single control region of 797 bp. AT ratio is 83.8%. 88 SNPs and 4 INDELs (175 bp) were identified against mitogenome of A. gossypii isolated from cotton species. Especially, largest INDEL (170 bp) was in the control region. Phylogenetic trees show that four A. gossypii mitogenomes were clustered in one clade.

A New Mitochondrial Genome of Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) and Mitogenome-Wide Investigation on Polymorphisms

“…Furthermore, the numbers of intraspecific variations in WBPHs were also smaller than those of Aphis gossypii Glover [61,94,95] and Spodoptera frugiperda (J. E. Smith) [64] but were larger in number than those of Korean samples of Hipparchia autonoe (Esper) [96] and Alphitobius diaperinus Panzer [58].…”

“…Pair-wise sequence alignments among complete mitogenome of WBPH including the mitogenome sequenced in this study were conducted by MAFFT v7.450 [56] with the default option. Identifying intraspecific variations, SNPs and INDELs, on the WBPH mitogenomes was conducted using the function, 'Find variations/SNPs,' implemented in the Geneious Prime ® v2020.2.4 (Biomatters Ltd., Auckland, New Zealand), which has been utilized in the previous insect mitochondrial genome studies [37,[57][58][59][60][61][62][63][64]. When the number of INDELs was calculated, continuous INDEL bases were considered one INDEL region.…”

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“…Mitochondrial genome sequences have been utilized for understanding phylogenetic relationships [30][31][32][33] as well as distinguishing population structures [34][35][36][37][38][39][40][41] because invertebrate mitochondrial genomes have a well-conserved structure [42] and keep a high copy number in the cell [43], which is favorable for assembling the mitochondrial genome from NGS raw reads. In addition, a short length of the mitochondrial genome reduces the costs to investigate intraspecific variations in the mitochondrial genomes [33][34][35][36][38][39][40][44][45][46][47][48][49][50][51][52][53][54][55][56]. The current available mitochondrial genomes of A. japonicus are also enough to investigate its intraspecific characteristics in detail; however, there is no systematic bioinformatic analysis of all available A. japonicus mitochondrial genomes till now.…”

“…Sogatella furcifera (43 bp from three mitochondrial genomes; Park et al, in submission)[78], Laodelphax striatellus (155 bp from eighty-seven mitochondrial genomes)[34,35,37], and Aphis gossypii (186 bp from five mitochondrial genomes)[46,48,52,55,56] except that of Nilaparvata lugens (14 bp from the five mitochondrial genomes excluding them without control regions)[38][39][40]79](Park et al, in submission). It implies that intraspecific differences of A.…”

The complete chloroplast genome of Castanopsis sieboldii (Makino) Hatus (Fagaceae)

“…The voucher was deposited in the InfoBoss Cyber Herbarium (IN; http://herbarium.infoboss.co.kr/ ; Voucher number: IB-01087; Contact: Suhyeon Park; shpark817@infoboss.co.kr ). Genome sequencing was conducted using NovaSeq6000 at Macrogen Inc., Korea, and de novo assembly and sequence confirmation were done by Velvet v1.2.10 (Zerbino and Birney 2008 ), GapCloser v1.12 (Zhao et al 2011 ), BWA v0.7.17 (Li 2013 ), and SAMtools v1.9 (Li et al 2009 ) in the Genome Information System (GeIS; http://geis.infoboss.co.kr/ ) which has been utilized in the previous organelle genomic studies (Park, Kim, Xi, Nho, et al 2019 ; Park, Park, Kim, et al 2019 ; Park, Yun, Oh, et al 2019 ; Park, Lee, et al 2021 ; Park, Min, et al 2021 ; Joo et al 2020 ; Park and Oh 2020 ; Kim et al 2021 ). Geneious Prime ® v2020.2.4 (Biomatters Ltd., Auckland, New Zealand) was used for chloroplast genome annotation based on Castanopsis fargesii chloroplast (NC_047230; Ye et al 2019 ).…”