The complete mitochondrial genome of a tropical midge Chironomus kiiensis Tokunaga, 1936 (Diptera: Chironomidae)

Abstract

In this study, we presented the complete mitochondrial genome of a tropical midge Chironomus kiiensis Tokunaga by Illumina sequencing technology. The complete mitogenome of C. kiiensis is 15,710 bp in length, including 13 protein-coding genes (PCGs), two ribosomal RNAs and 22 tRNAs. The overall nucleotide composition is A: 39.3%, T: 37.6%, C: 13.6%, and G: 9.5%. Most PCGs use ATN as the start codon excluding COX1 (TTG), and most PCGs use TAA as the stop codon excluding ND4 (TAG). Gene order of the C. kiiensis mitogenome is identical to those of other Chironomidae mitogenomes. Phylogenetic analysis based on the mitogenomic PCGs indicate that Chironomus is a sister clade to Polypedilum .

Codon bias analysis of the mitochondrial genome reveals natural selection in the nonbiting midge Microtendipes umbrosus Freeman, 1955 (Diptera: Chironomidae)

“…AT-skew analysis reveals T-biased PCGs and rRNAs, while tRNAs exhibited an A-biased pattern (Table 2). As expected, M. umbrosus shares similar mitogenomic features with other Chironomidae species, such as Chironomus kiiensis Tokunaga, 1936 and Polypedilum unifascium Tokunaga, 1938 (Lei et al 2021, Liu et al 2022). Chironomidae mitogenomes present stability, maintaining similar gene content, gene arrangement, and base composition.…”

Comparative Analysis of Mitogenomes of Chironomus (Diptera: Chironomidae)

“…Hence, phylogenetics among Chironomus has never been tested based on mitogenomes. Prior to this study, the mitogenomes of three Chironomus species have been reported [19][20][21], and the comparative analysis of nucleotide composition and evolutionary rates within the genus have never been carried out.…”

Complete mitochondrial genome of the non-biting midge Polypedilum henicurum Wang, 1995 (Diptera: Chironomidae)

“… 2022 ), MZ150770 (Liu et al. 2022 ), MZ261913 (Kong et al. 2021 ), MZ981735 (this study), MW677959 (Lei et al.…”