The complete mitochondrial genome of the New Zealand parasitic roundwormTeladorsagia circumcincta(Trichostrongyloidea: Haemonchidae) field strain NZ_Teci_NP

Abstract

The complete mitochondrial genome of the New Zealand parasitic nematode Teladorsagia circumcincta field strain NZ_Teci_NP was sequenced and annotated. The 14,083 bp-long mitogenome contains 12 protein-coding genes (atp8 gene missing), two ribosomal RNAs (rRNAs), and 22 transfer RNAs (tRNAs). Phylogenetic analysis showed that T. circumcincta NZ_Teci_NP forms a monophyletic cluster with the remaining Haemonchidae species and further reinforces the high levels of diversity and gene flow observed among Trichostrongylidae.

Characterization of the complete mitochondrial genomes of two sibling species of parasitic roundworms, Haemonchus contortus and Teladorsagia circumcincta.

“…The acquisition of parasite samples, DNA extraction, and genome sequencing technologies used to generate the mt genomes used for analysis in this study have recently been outlined in the form of brief reports [13,14]. To ll any knowledge gaps associated with the above-mentioned reports, here we describe in detail the methodologies, sequencing technologies and annotation software used to generate the H. contortus NZ_Hco_NP and T. circumcincta NZ_Teci_NP mt genomes.…”

“…To ll any knowledge gaps associated with the above-mentioned reports, here we describe in detail the methodologies, sequencing technologies and annotation software used to generate the H. contortus NZ_Hco_NP and T. circumcincta NZ_Teci_NP mt genomes. H. contortus and T. circumcincta were recovered from sheep infected with pure strains of parasites in Palmerston North, New Zealand, and total high molecular weight genomic DNA was extracted separately from multiple worms using a modi ed phenol:chloroform protocol, as previously described [13,14,16,29]. DNA was deposited, stored and available upon request from AgResearch Ltd., Grasslands Research Centre.…”

“…The complete mitochondrial genome sequences of H. contortus NZ_Hco_NP [13,16] and T. circumcincta NZ_Teci_NP [14] were obtained using next-generation sequencing technologies. The H. contortus NZ_Hco_NP (BioProject ID: PRJNA517503, SRA accession number SRP247265) whole genome shotgun paired-end (PE) and single-molecule, real-time (SMRT) long-read libraries (SRA Runs: SRR11022845 and SRR11022846) were generated using the Illumina HiSeq2500 and Paci c Biosciences (PacBio) platforms, as previously described [13,16].…”

“…The development of low cost, high-throughput, next-generation sequencing technologies have led to a recent focus on whole-genome sequencing (WGS) of complete nuclear genomes of parasitic roundworms (Nematodes) [11][12][13][14][15], instead of earlier markers such as cox1, cox2, and nad genes. Owing to the substantial coverage following WGS [16,17] and advances in bioinformatics pipelines, further complete mitochondrial genome resources will be available to facilitate detailed comparative phylogenetic analyses across many species complexes and major taxonomic groups of nematodes [18,19].…”

“…For these reasons, this study interrogates the complete mitochondrial genome sequences of the anthelmintic-susceptible H. contortus NZ_Hco_NP [13] and T. circumcincta NZ_Teci_NP [14] derived from pasture-grazed New Zealand sheep. In this study, we performed numerous comparative and phylogenomic analyses to directly compare the molecular characteristics including the nucleotide composition and codon usage pro les of PCGs, as well as the secondary structures of each identi ed tRNA and rRNA gene within the two species, but also among other closely related trichostrongyloid nematodes.…”