The complete mitochondrial genome of the neotropical helicopter damselflyMegaloprepus caerulatus(Odonata: Zygoptera) assembled from next generation sequencing data

Abstract

Odonata (dragonflies and damselflies) is a small order at the base of flying insects (Pterygota). Resolving family-level phylogenetic relationships within this order receives great attention. Hereby, genetic data already resulted in various changes, which are however still under discussion. Mitochondrial genomes may further enhance such phylogenies. This study presents the complete mitochondrial genome of the Neotropical damselfly Megaloprepus caerulatus based on next generation sequencing (NGS) data on total genomic DNA. The total length comprises 16,094 bp and includes the standard metazoan set of 37 genes together with a 1376 bp long A þ T rich (control) region. Gene content, gene arrangement and base frequency are consistent with other odonate mitochondrial genomes. It further contains four intergenic spacer regions, indicating a possible family specific feature for the Coenagrionidae and its close relatives.

The characteristics and phylogenetic relationship of two complete mitochondrial genomes of Matrona basilaris (Odonata: Zygoptera: Calopterygidae)

“…The two complete mitochondrial genomes of M. basilaris are 16,149 bp (JH) and 15,893 bp (TMS) in length, respectively. All protein-coding genes of the two samples are AT-biased (68.9%), with atp8 showing the highest A þ T content (76.8% and 76.1%) whereas cox1 has the lowest A þ T content (both samples are 63%); these results are similar to other odonates (Lin et al 2010;Lorenzo-Carballa et al 2014;Feindt et al 2016). All protein-coding genes of M. basilaris employ the characteristic invertebrate specific mitochondrial start codons: cox2, cox3, atp6, nad4, nad4L, cytb and nad1 start with ATG; cox1 and nad3 with ATA; atp8 and nad5 with ATT; and nad2 and nad6 with ATC.…”

“…We constructed Bayesian inference (BI) and maximum likelihood (ML) trees from the 13 PCGs of M. basilaris and 28 other Odonata species (Yamauchi et al 2004;Lin et al 2010;Lorenzo-Carballa et al 2014;Tang et al 2014;Feindt et al 2016;Yong et al 2016;Yu et al 2016;Zhang et al 2017) and using four outgroups: Isonychia kiangsinensis (Ye et al 2018), Epeorus herklotsi , Siphluriscus chinensis and Caenis sp. (Figure 1).…”

Some mitochondrial genes perform better for damselfly phylogenetics: species‐ and population‐level analyses of four complete mitogenomes of Euphaea sibling species

“…; Feindt et al . , b) will greatly facilitate the development of taxon‐specific nad2 and A + T‐rich region primers in diverse damselfly species. Determining the phylogenetic utility of these two mitochondrial markers in other damselflies requires further studies.…”

The complete mitochondrial genome sequence ofAcisoma panorpoidesRambur, 1842 (Odonate: Libellulidae)

“…Acisoma panorpoides encoded an A þ T-rich control region and a typical set of 37 mitochondrial genes, including 13 proteincoding genes, 2 rRNA genes, 22 tRNA genes. The arrangement and orientation of the mitochondrial genes are identical to the other odonate mitochondrial genomes (Simon and Hadrys 2013;Wang et al 2015;Feindt et al 2016). The base composition of the mitochondrial genome is A: 34.6%, T: 39.8%, C: 11.1% and G: 14.5%, with an A þ T content of 74.4%.…”

“…The mitochondrial genome was assembled using SOAPdenovo v2.04 (http://soap.genomics. org.cn/) and annotated via available mitochondrial genomes of other odonate species (Lorenzo-Carballa et al 2014;Yu et al 2014;Feindt et al 2016;Herzog et al 2016). The sequences of the other 18 odonate mitochondrial genomes and Parafronurus youi were downloaded from GenBank and were used to construct the minimum evolutionary tree (10000 replicates) using MEGA X (Kumar et al 2018).…”