The complete mitochondrial genome of Trabala vishnou guttata (Lepidoptera: Lasiocampidae) and the related phylogenetic analyses

Abstract

The bluish yellow lappet moth, Trabala vishnou guttata is an extraordinarily important pest in China. The complete mitochondrial genome is sequenced and determined firstly, which is based on traditional PCR amplification and primer walking methods with a length of 15,281 bp, including 13 protein-coding (PCG) genes, 22 transfer RNA (rRNA) genes, two ribosomal RNA (tRNA) genes, and an A + T-rich region. The gene order and orientation of the T. vishnou guttata mitogenome were identical to the other sequenced Lasiocampidae species. The overall nucleotide composition of T. vishnou guttata is A (40.27 %), T (40.59 %), C (11.58 %) and G (7.56 %), respectively. All the PCGs initiate with the three orthodox start codons ATN except for coxI with CGA start codon. Three PCGs (coxI, coxII and nad4) used incomplete stop codon T, while the other 10 PCGs terminate with complete stop codon TAA. All tRNA genes have a typical clover-leaf structure except for the absence of a dihydrouridine arm in trnS (AGN). The length of A + T-rich region is 383 bp. Phylogeny is established to reveal the genetic relationship between T. vishnou guttata and other lepidopteran species based on 13 PCGs nucleotide sequences of the sequenced species (32 taxa) by Maximum likelihood and Bayesian methods. Phylogenetic analyses presents that T. vishnou guttata and its closely related species (Dendrolimus taxa) are clustered on Lasiocampidae group. It is a sister clade relationship between Lasiocampidae and other families in Bombycoidea with a bootstrap value of 83 % and a posterior probability of 0.75. This study supports that Lasiocampidae may be independent from Bombycoidea.

The complete mitochondrial genome of Dendrolimus kikuchii (Lepidoptera: Lasiocampidae)

“…Furthermore, the phylogenetic position of D. kikuchii among the genus Dendrolimus was D. kikuchiiþ (D. spectabilisþ (D. tabulaeformis þ D. punctatus)), which was supported as a monophyletic clade by a posterior probability of 1.00 and a bootstrap value of 100%. The evolutionary relationships of these analysed species are consistent with previously reported results (Kononov et al 2016;Min et al 2016;Wu et al 2016aWu et al , 2016b. The newly determined mitogenome is useful for understanding the evolution of the pine moths.…”

“…2016 ; Wu et al. 2016a , 2016b ). The newly determined mitogenome is useful for understanding the evolution of the pine moths.…”

Mitochondrial phylogeny and comparative mitogenomics of closely related pine moth pests (Lepidoptera:Dendrolimus)

“…The monophyly of each superfamily was generally well-supported based on the 78 mitogenome analysis, which was consistency with prior studies (Yang et al, 2009; Kawahara & Breinholt, 2014; Qin et al, 2015). Previous studies have included Lasiocampidae within Bombycoidea (Brock, 1971; Scoble, 1992; Kawahara & Breinholt, 2014), while other studies have treated Lasiocampidae as a distinct superfamily Lasiocampoidea (Minet, 1991; Regier et al, 2009; Van Nieukerken et al, 2011; Zwick et al, 2011; Bazinet et al, 2013; Wu et al, 2016). However, according to our results based on mitogenomes, Lasiocampoidea, and Bombycoidea were monophyletic and clustered together as sister groups with high support.…”