The complete mitochondrial genome of the New Zealand parasitic blowfly <i>Lucilia sericata</i> (Insecta: Diptera: Calliphoridae)

Palevich, Nikola; Carvalho, Luis; Maclean, Paul

doi:10.1080/23802359.2021.1906774

Cited by 9 publications

(6 citation statements)

References 17 publications

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“…Initially identified in Hawaii [12,43,64], L. c. cuprina has since been documented in Australia [9], North America [39], South Africa [52], and Southeast Asia [65]. The phylogenetic relationships inferred here within the family Calliphoridae using mt datasets support those reported in previous investigations [8,9,66].…”

Section: Discussionsupporting

confidence: 82%

Complete Mitochondrial Genome for Lucilia cuprina dorsalis (Diptera: Calliphoridae) from the Northern Territory, Australia

Kapoor,

Yang,

Hall

et al. 2024

Genes

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The Australian sheep blowfly, Lucilia cuprina dorsalis, is a major sheep ectoparasite causing subcutaneous myiasis (flystrike), which can lead to reduced livestock productivity and, in severe instances, death of the affected animals. It is also a primary colonizer of carrion, an efficient pollinator, and used in maggot debridement therapy and forensic investigations. In this study, we report the complete mitochondrial (mt) genome of L. c. dorsalis from the Northern Territory (NT), Australia, where sheep are prohibited animals, unlike the rest of Australia. The mt genome is 15,943 bp in length, comprising 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and a non-coding control region. The gene order of the current mt genome is consistent with the previously published L. cuprina mt genomes. Nucleotide composition revealed an AT bias, accounting for 77.5% of total mt genome nucleotides. Phylogenetic analyses of 56 species/taxa of dipterans indicated that L. c. dorsalis and L. sericata are the closest among all sibling species of the genus Lucilia, which helps to explain species evolution within the family Luciliinae. This study provides the first complete mt genome sequence for L. c. dorsalis derived from the NT, Australia to facilitate species identification and the examination of the evolutionary history of these blowflies.

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Section: Discussionsupporting

confidence: 82%

Complete Mitochondrial Genome for Lucilia cuprina dorsalis (Diptera: Calliphoridae) from the Northern Territory, Australia

Kapoor,

Yang,

Hall

et al. 2024

Genes

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“…The L. cuprina mt genomes included here are in the size range of previously published mt genomes: L. cuprina strain DI213.5 (GenBank accession number: JX913753) 15,226 bp, L. cuprina strain DI213.2 (JX913750) 15,310 bp, L. cuprina strain DI190.5 (JX913748) 15,946 bp, and L. cuprina strain DI190.1 (JX913744) 15,952 bp [17]). The lengths of previously sequenced L. sericata mt genomes are as follows: L. sericata strain DI257 (JX913757) 15,380 bp, L. sericata strain DI245 (JX913756) 15,214 bp, L. sericata strain DI220 (JX913755) 15,300 bp, L. sericata strain DI246 (JX913754) 15,243 bp [17], L. sericata (NPY120886) 15938 bp [45] and L. sericata (AJ422212) 15,945 bp [46]. Based on the predicted annotation, each complete mt genome characterised herein included 37 genes consisting of 13 PCGs, 2 rRNAs [small (rrnS) and large (rrnL)], 22 tRNAs and a control region (Additional le 3: Table S3, Fig.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial genomic investigation reveals a clear association between species and genotypes of Lucilia and geographic origin in Australia

Kapoor

Young

Yang

et al. 2023

Preprint

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Background Lucilia cuprina and L. sericata (family Calliphoridae) are globally significant ectoparasites of sheep. Current literature suggests that only one of these blowfly subspecies, L. cuprina dorsalis, is a primary parasite causing myiasis (flystrike) in sheep in Australia. These species and subspecies are difficult to distinguish using morphological features. Hence, being able to accurately identify blowflies is critical for diagnosis and for understanding their relationships with their hosts and environment.Methods In this study, adult blowflies (5 pools of 17 flies; n = 85) were collected from five locations in different states [New South Wales (NSW), Queensland (QLD), Tasmania (TAS), Victoria (VIC) and Western Australia (WA)] of Australia and their mitochondrial (mt) genomes were assembled.Results Each mt genome assembled was ~ 15 kb in size and encoded 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and a control region. The Lucilia species mt genomes were conserved in structure and the genes retained the same order and direction. The overall nucleotide composition was heavily biased towards As and Ts − 77.7% of the whole genomes. Pairwise nucleotide diversity suggested divergence between L. cuprina cuprina, L. c. dorsalis and L. sericata. Comparative analyses of these mt genomes with published data demonstrated that the blowflies collected from sheep farm in TAS clustered within a clade with L. sericata. The flies collected from an urban location in QLD were more closely related to L. sericata and represented the subspecies L. c. cuprina, whereas the flies collected from sheep farms in NSW, VIC and WA represented the subspecies L. c. dorsalis.Conclusions Phylogenetic analyses of the mt genomes representing Lucilia from the five geographic locations in Australia supported the previously demonstrated paraphyly of L. cuprina with respect to L. sericata and revealed that L. c. cuprina is distinct from L. c. dorsalis, and that L. c. cuprina is more closely related to L. sericata than L. c. dorsalis. The mt genomes reported here provide an important molecular resource to develop tools for species- and subspecies-level identification of Lucilia from different geographical regions across Australia.

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“…The L. cuprina mt genomes included here are in the size range of previously published mt genomes: L. cuprina strain DI213.5 (GenBank accession number: JX913753) 15,226 bp, L. cuprina strain DI213.2 (JX913750) 15,310 bp , L. cuprina strain DI190.5 (JX913748) 15,946 bp and L. cuprina strain DI190.1 (JX913744) 15,952 bp [ 17 ]). The lengths of previously sequenced L. sericata mt genomes are as follows: L. sericata strain DI257 (JX913757) 15,380 bp, L. sericata strain DI245 (JX913756) 15,214 bp, L. sericata strain DI220 (JX913755) 15,300 bp, L. sericata strain DI246 (JX913754) 15,243 bp [ 17 ], L. sericata (NPY120886) 15,938 bp [ 50 ] and L. sericata (AJ422212) 15,945 bp [ 51 ]. Based on the predicted annotation, each complete mt genome characterised herein included 37 genes consisting of 13 PCGs, 2 rRNAs [small (rrnS) and large (rrnL)], 22 tRNAs and a control region (Additional file 3 : Table S3, Fig.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial genomic investigation reveals a clear association between species and genotypes of Lucilia and geographic origin in Australia

et al. 2023

View full text Add to dashboard Cite

Background Lucilia cuprina and L. sericata (family Calliphoridae) are globally significant ectoparasites of sheep. Current literature suggests that only one of these blowfly subspecies, L. cuprina dorsalis, is a primary parasite causing myiasis (flystrike) in sheep in Australia. These species and subspecies are difficult to distinguish using morphological features. Hence, being able to accurately identify blowflies is critical for diagnosis and for understanding their relationships with their hosts and environment. Methods In this study, adult blowflies (5 pools of 17 flies; n = 85) were collected from five locations in different states [New South Wales (NSW), Queensland (QLD), Tasmania (TAS), Victoria (VIC) and Western Australia (WA)] of Australia and their mitochondrial (mt) genomes were assembled. Results Each mt genome assembled was ~ 15 kb in size and encoded 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and a control region. The Lucilia species mt genomes were conserved in structure, and the genes retained the same order and direction. The overall nucleotide composition was heavily biased towards As and Ts—77.7% of the whole genomes. Pairwise nucleotide diversity suggested divergence between Lucilia cuprina cuprina, L. c. dorsalis and L. sericata. Comparative analyses of these mt genomes with published data demonstrated that the blowflies collected from sheep farm in TAS clustered within a clade with L. sericata. The flies collected from an urban location in QLD were more closely related to L. sericata and represented the subspecies L. c. cuprina, whereas the flies collected from sheep farms in NSW, VIC and WA represented the subspecies L. c. dorsalis. Conclusions Phylogenetic analyses of the mt genomes representing Lucilia from the five geographic locations in Australia supported the previously demonstrated paraphyly of L. cuprina with respect to L. sericata and revealed that L. c. cuprina is distinct from L. c. dorsalis and that L. c. cuprina is more closely related to L. sericata than L. c. dorsalis. The mt genomes reported here provide an important molecular resource to develop tools for species- and subspecies-level identification of Lucilia from different geographical regions across Australia. Graphical Abstract

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The complete mitochondrial genome of the New Zealand parasitic blowfly Lucilia sericata (Insecta: Diptera: Calliphoridae)

Cited by 9 publications

References 17 publications

Complete Mitochondrial Genome for Lucilia cuprina dorsalis (Diptera: Calliphoridae) from the Northern Territory, Australia

Complete Mitochondrial Genome for Lucilia cuprina dorsalis (Diptera: Calliphoridae) from the Northern Territory, Australia

Mitochondrial genomic investigation reveals a clear association between species and genotypes of Lucilia and geographic origin in Australia

Mitochondrial genomic investigation reveals a clear association between species and genotypes of Lucilia and geographic origin in Australia

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