The complete mitochondrial genome of red frog crab Ranina ranina (Crustacea: Decapoda: Brachyura: Raninidae)

Abstract

Although the brachyuran nature of Raninoidea is widely accepted, there is no consensus over the precise position of the Raninoidea within Brachyura. Long PCR and primer walking methods are employed to determine the first complete mitochondrial genome (mitogenome) sequence of raninoidian crab, Ranina ranina. It is a circular double-stranded DNA molecule of 15,563 base pairs (bp) in length with a standard set of 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), 13 protein-coding genes (PCGs) as well as a putative non-coding control region. The gene order is substantially consistent with that of the pancrustacean ground pattern with the tRNA(His) gene rearrangement. The basal placement of R. ranina in the phylogenetic tree integrated with a similar genomic organization to ancestral pancrustacea confirmed the primitive position of R. ranina in the Brachyura.

The complete mitogenome of blue swimming crab Portunus pelagicus Linnaeus, 1766 (Crustacea: Decapoda: Portunidae)

“…Twenty-three of the genes are transcribed from the heavy strand, whereas the remainder are encoded on the light strand. The overall A + T content of P. pelagicus mtDNA is 68.79% (33.70% A, 18.99% C, 12.22% G, and 35.09% T), similar to that of other brachyurans (Cheng et al, 2014;Yu et al, 2014), and this pattern of base composition holds for the protein-coding, rRNA, and tRNA genes, as well as the control region. Typically the tRNA His gene is located between ND5 and ND4 in the ancestral pancrustacea, while in P. pelagicus it lies between tRNA Glu and tRNA Phe .…”