The complete mitochondrial genome of <i>Gyrodactylus gurleyi</i> (Platyhelminthes: Monogenea)

Zou, Hong; Zhang, Dong; Li, Wenxiang; Zhou, Shun; Wu, Shangong; Wang, Guitang

doi:10.1080/23802359.2016.1172042

Cited by 8 publications

(6 citation statements)

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“…1 ). The LNCR of L. longipenis (1993 bp) was the longest among the monogeneans characterized so far [ 4 , 7 , 16 , 19 , 20 , 50 , 51 , 59 – 67 ]. The A+T content of both LNCRs ( L. longipenis = 93.8%, L. spari = 92.5%) was much higher than in other parts of the mitogenomes (Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial genomes of two diplectanids (Platyhelminthes: Monogenea) expose paraphyly of the order Dactylogyridea and extensive tRNA gene rearrangements

Zhang

Zou

et al. 2018

Parasites Vectors

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BackgroundRecent mitochondrial phylogenomics studies have reported a sister-group relationship of the orders Capsalidea and Dactylogyridea, which is inconsistent with previous morphology- and molecular-based phylogenies. As Dactylogyridea mitochondrial genomes (mitogenomes) are currently represented by only one family, to improve the phylogenetic resolution, we sequenced and characterized two dactylogyridean parasites, Lamellodiscus spari and Lepidotrema longipenis, belonging to a non-represented family Diplectanidae.ResultsThe L. longipenis mitogenome (15,433 bp) contains the standard 36 flatworm mitochondrial genes (atp8 is absent), whereas we failed to detect trnS1, trnC and trnG in L. spari (14,614 bp). Both mitogenomes exhibit unique gene orders (among the Monogenea), with a number of tRNA rearrangements. Both long non-coding regions contain a number of different (partially overlapping) repeat sequences. Intriguingly, these include putative tRNA pseudogenes in a tandem array (17 trnV pseudogenes in L. longipenis, 13 trnY pseudogenes in L. spari). Combined nucleotide diversity, non-synonymous/synonymous substitutions ratio and average sequence identity analyses consistently showed that nad2, nad5 and nad4 were the most variable PCGs, whereas cox1, cox2 and cytb were the most conserved. Phylogenomic analysis showed that the newly sequenced species of the family Diplectanidae formed a sister-group with the Dactylogyridae + Capsalidae clade. Thus Dactylogyridea (represented by the Diplectanidae and Dactylogyridae) was rendered paraphyletic (with high statistical support) by the nested Capsalidea (represented by the Capsalidae) clade.ConclusionsOur results show that nad2, nad5 and nad4 (fast-evolving) would be better candidates than cox1 (slow-evolving) for species identification and population genetics studies in the Diplectanidae. The unique gene order pattern further suggests discontinuous evolution of mitogenomic gene order arrangement in the Class Monogenea. This first report of paraphyly of the Dactylogyridea highlights the need to generate more molecular data for monogenean parasites, in order to be able to clarify their relationships using large datasets, as single-gene markers appear to provide a phylogenetic resolution which is too low for the task.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3144-6) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Mitochondrial genomes of two diplectanids (Platyhelminthes: Monogenea) expose paraphyly of the order Dactylogyridea and extensive tRNA gene rearrangements

Zhang

Zou

et al. 2018

Parasites Vectors

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“…So far only nine gyrodactylid [ 21 , 30 – 36 ] and two dactylogyrid [ 37 , 38 ] monogenean mitogenomes have been published. Our study substantially increases the quantity of available mitogenomic data on these two most diverse monogenean families, by one-third, and offers the first mitogenomes from African representatives.…”

Section: Discussionmentioning

confidence: 99%

“…To situate the African monogeneans under study within their respective families, the PCGs and rRNA genes of all available dactylogyrid [ 37 , 38 ] and gyrodactylid [ 21 , 30 – 36 ] mitogenomes were included in phylogenetic analyses. The species of Capsalidae for which mitogenomes are available [ 69 , 100 , 101 ] were also included as they strongly cluster with the dactylogyrids [ 21 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)

et al. 2018

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BackgroundMonogenean flatworms are the main ectoparasites of fishes. Representatives of the species-rich families Gyrodactylidae and Dactylogyridae, especially those infecting cichlid fishes and clariid catfishes, are important parasites in African aquaculture, even more so due to the massive anthropogenic translocation of their hosts worldwide. Several questions on their evolution, such as the phylogenetic position of Macrogyrodactylus and the highly speciose Gyrodactylus, remain unresolved with available molecular markers. Also, diagnostics and population-level research would benefit from the development of higher-resolution genetic markers. We aim to offer genetic resources for work on African monogeneans by providing mitogenomic data of four species (two belonging to Gyrodactylidae, two to Dactylogyridae), and analysing their gene sequences and gene order from a phylogenetic perspective.ResultsUsing Illumina technology, the first four mitochondrial genomes of African monogeneans were assembled and annotated for the cichlid parasites Gyrodactylus nyanzae, Cichlidogyrus halli, Cichlidogyrus mbirizei (near-complete mitogenome) and the catfish parasite Macrogyrodactylus karibae (near-complete mitogenome). Complete nuclear ribosomal operons were also retrieved, as molecular vouchers. The start codon TTG is new for Gyrodactylus and for Dactylogyridae, as is the incomplete stop codon TA for Dactylogyridae. Especially the nad2 gene is promising for primer development. Gene order was identical for protein-coding genes and differed between the African representatives of these families only in a tRNA gene transposition. A mitochondrial phylogeny based on an alignment of nearly 12,500 bp including 12 protein-coding and two ribosomal RNA genes confirms that the Neotropical oviparous Aglaiogyrodactylus forficulatus takes a sister group position with respect to the other gyrodactylids, instead of the supposedly ‘primitive’ African Macrogyrodactylus. Inclusion of the African Gyrodactylus nyanzae confirms the paraphyly of Gyrodactylus. The position of the African dactylogyrid Cichlidogyrus is unresolved, although gene order suggests it is closely related to marine ancyrocephalines.ConclusionsThe amount of mitogenomic data available for gyrodactylids and dactylogyrids is increased by roughly one-third. Our study underscores the potential of mitochondrial genes and gene order in flatworm phylogenetics, and of next-generation sequencing for marker development for these non-model helminths for which few primers are available.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4893-5) contains supplementary material, which is available to authorized users.

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“…So far only nine gyrodactylid (Huyse et al 2007, 2008; Plaisance et al 2007; Ye et al 2014, 2017; Bachmann et al 2016; Zhang et al 2016; Zou et al 2016) and two dactylogyrid (Zhang et al 2016, 2017) monogenean mitogenomes have been published. Our study substantially increases the quantity of available mitogenomic data on these two most diverse monogenean families, by one-third, and offers the first mitogenomes from African representatives.…”

Section: Discussionmentioning

confidence: 99%

“…To situate the African monogeneans under study within their respective families, the PCGs and rRNA genes of all available dactylogyrid (Zhang et al 2016, 2017) and gyrodactylid (Huyse et al 2007, 2008; Plaisance et al 2007; Ye et al 2014, 2017; Bachmann et al 2016; Zhang et al 2016; Zou et al 2016) mitogenomes were included in phylogenetic analyses. The species of Capsalidae, also monopisthocotylean monogeneans, for which mitogenomes are available (Perkins et al 2010; Kang et al 2012; Zhang et al 2014) were used as outgroup.…”

Section: Methodsmentioning

confidence: 99%

The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)

Vanhove

Briscoe

Jorissen

et al. 2018

Preprint

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The complete mitochondrial genome of Gyrodactylus gurleyi (Platyhelminthes: Monogenea)

Cited by 8 publications

References 9 publications

Mitochondrial genomes of two diplectanids (Platyhelminthes: Monogenea) expose paraphyly of the order Dactylogyridea and extensive tRNA gene rearrangements

Mitochondrial genomes of two diplectanids (Platyhelminthes: Monogenea) expose paraphyly of the order Dactylogyridea and extensive tRNA gene rearrangements

The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)

The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae)

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