The complete mitochondrial genome of the Garra tibetana (Cypriniformes: Cyprinidae)

Abstract

The Garra tibetana, an important economic fish belonging to the genus Garra, family Cyprinidae, is mainly distributed in Tibet with rare biological resources. Here, we sequenced and characterized the complete mitochondrial genome of G. tibetana for the first time, and the results showed that the total length of G. tibetana mitogenome was 16,861 bp, consisting of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 2 control regions. Unlike the typical mitochondrial genome organization in vertebrate, the D-loop region was separated into two part by a tRNA Pro gene. To further explore the phylogenetic relationship of the G. tibetana, we constructed the phylogenetic tree and verified that the G. tibetana was a new species of the genus Garra and had a closer relationship with Garra kempi. This study not only provided valuable evidence on phylogenetic relationship of the G. tibetana at the molecular level, but also provided an essential resource for further population genetics research and germplasm conservation on this species. ARTICLE HISTORY

Novel Gene Rearrangement in the Mitochondrial Genome of Three Garra and Insights Into the Phylogenetic Relationships of Labeoninae

“…They feed on plant debris in open-water habitats and on periphyton in bottom-surface habitats ( Kullander and Fang, 2004 ; Nebeshwar et al, 2009 ). Currently, 20 different Garra species have been identified from the Brahmaputra River basin, three of which in China, namely, G. dengba , G. Tibetan , and G. yajiangensis caught our attention due to the rearrangement in their mitochondrial genome ( Deng et al, 2018 ; Gong, 2018 ; Gong et al, 2018 ; Zhang et al, 2020 ). They were all collected from high altitudes.…”

“…G. yajiangensis , a member of the long-nosed species group, is distinguished from other members of the group mainly by the presence of a prominent, quadrangular, slightly bilobed proboscis. Apart from G. tibetana has been reported as a complete mitochondrial sequence, current researches on these three Garra fishes are mainly stagnant in morphology while their corresponding molecular evolutions are still limited ( Zhang et al, 2020 ). In addition, Zhang et al (2020) identified two control regions in the mitochondrial genome of G. tibetana ( Zhang et al, 2020 ).…”

“…Apart from G. tibetana has been reported as a complete mitochondrial sequence, current researches on these three Garra fishes are mainly stagnant in morphology while their corresponding molecular evolutions are still limited ( Zhang et al, 2020 ). In addition, Zhang et al (2020) identified two control regions in the mitochondrial genome of G. tibetana ( Zhang et al, 2020 ). Also, Li et al (2016) reported that the tRNA-Pro gene of G. kempi is positioned between two control regions, and a 246 bp repeat unit is identified in the second control region, which is different from the typical mitochondrial genome organization in vertebrate ( Li et al, 2016 ).…”