The complete mitochondrial genome of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae: gene order and trn gene clusters reveal a common evolutionary course for all Sordariomycetes, while intergenic regions show variation

Ghikas, Dimitri V.; Kouvelis, Vassili N.; Typas, Milton A.

doi:10.1007/s00203-006-0104-x

Cited by 59 publications

(95 citation statements)

References 45 publications

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“…A distinctive feature of fungal mitochondrial genomes is the clustering of tRNA genes (Ghikas et al, 2006), and it is thought that both the tRNA gene content and their placement will be conserved in fungi (Table 4). The 27 tRNA genes clustered into five groups, with the two larger tRNA gene clusters flanking rnl, a pattern common to other fungal mitochondrial DNAs (mtDNAs) (Tambor et al, 2006).…”

Section: The Mitochondrial Genomementioning

confidence: 99%

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

et al. 2007

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Stagonospora nodorum is a major necrotrophic fungal pathogen of wheat (Triticum aestivum) and a member of the Dothideomycetes, a large fungal taxon that includes many important plant pathogens affecting all major crop plant families. Here, we report the acquisition and initial analysis of a draft genome sequence for this fungus. The assembly comprises 37,164,227 bp of nuclear DNA contained in 107 scaffolds. The circular mitochondrial genome comprises 49,761 bp encoding 46 genes, including four that are intron encoded. The nuclear genome assembly contains 26 classes of repetitive DNA, comprising 4.5% of the genome. Some of the repeats show evidence of repeat-induced point mutations consistent with a frequent sexual cycle. ESTs and gene prediction models support a minimum of 10,762 nuclear genes. Extensive orthology was found between the polyketide synthase family in S. nodorum and Cochliobolus heterostrophus, suggesting an ancient origin and conserved functions for these genes. A striking feature of the gene catalog was the large number of genes predicted to encode secreted proteins; the majority has no meaningful similarity to any other known genes. It is likely that genes for host-specific toxins, in addition to ToxA, will be found among this group. ESTs obtained from axenic mycelium grown on oleate (chosen to mimic early infection) and late-stage lesions sporulating on wheat leaves were obtained. Statistical analysis shows that transcripts encoding proteins involved in protein synthesis and in the production of extracellular proteases, cellulases, and xylanases predominate in the infection library. This suggests that the fungus is dependant on the degradation of wheat macromolecular constituents to provide the carbon skeletons and energy for the synthesis of proteins and other components destined for the developing pycnidiospores.

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Section: The Mitochondrial Genomementioning

confidence: 99%

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

et al. 2007

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“…Both CRcox1 and cox1 intron in C. militaris had very high AT bias, 69.3 and 75.0%, respectively. High AT bias was a feature of cox1 genes in other fungal mtDNA (Li et al 2008;Ghikas et al 2006). The molecular weights of deduced COX1 protein (530 aa) and putative LAGLI-DADG endonuclease (309 aa) were 58.14 and 35.49 kDa respectively, and the estimated pI values of them were 7.83 and 10.08 correspondingly.…”

Section: Sequence Analysis Of Cox1 Genementioning

confidence: 99%

Cordyceps militaris (Hypocreales: Cordycipitaceae): transcriptional analysis and molecular characterization of cox1 and group I intron with putative LAGLIDADG endonuclease

Zheng

Jiang

Huang

et al. 2011

World J Microbiol Biotechnol

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The full-length cytochrome c oxidase subunit I gene (cox1) containing a group I intron was isolated from an important medical fungus Cordyceps militaris (Cordycipitaceae). The open reading frame (ORF) of 1,593 nucleotides encoded a predicted protein COX1 of 530 amino acids. The group I intron encoded a putative homing endonuclease (HE) with two LAGLIDADG motifs. RT-PCR and Northern analysis showed a mature transcript of spliced cox1. Both 5'exon-intron and intron-3'exon junctions were also found by RT-PCR, suggesting the possible presence of unspliced cox1 RNA in total RNA. Sequence comparison by BLASTn showed that the coding region of cox1 (CRcox1) of C. militaris had significant similarities to those of related species (such as Cordyceps bassiana and C. brongniartii), while the intron had no significant homologous sequences of Cordycipitaceae fungi in NCBI database. The phylogenetic tree based on the CRcox1 confirmed the present taxonomic status of related species, but the cox1 introns were phylogenetically distinct. Compared to C. bassiana and C. brongniartii, the cox1 intron of C. militaris exhibited specific splicing site and different intronic ORF. The analysis of the folding RNA structures of the known cox1 introns from Cordyceps species showed different base pairs and conserved regions (P1-P10) in their structures. The present results provide useful information on the studies of cox1 intron splicing and Cordyceps evolution.

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“…Mitochondrial genome sizes are variable in fungi, and this is also reflected in the Clavicipitaceae where M. anisopliae mtDNA (accession number NC_008068; Ghikas, Kouvelis, & Typas, 2006) is 24,673 bp, C. purpurea mtDNA is estimated at 55,537 bp, E. bromicola E502 mtDNA is 69,466 bp, and E. festucae mtDNA is the largest at 72,701 bp. The size differences between these genomes are largely due to variations of intron number and size and the presence of a large variable region found in E2368 and E502 associated with the tRNA cluster containing 12 tRNA genes flanked by rnl/rps3 and nad2.…”

Section: Mitochondrial Genomesmentioning

confidence: 99%

Genomes of Plant-Associated Clavicipitaceae

Schardl

Young

Moore

et al. 2014

Advances in Botanical Research

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The complete mitochondrial genome of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae: gene order and trn gene clusters reveal a common evolutionary course for all Sordariomycetes, while intergenic regions show variation

Cited by 59 publications

References 45 publications

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

Cordyceps militaris (Hypocreales: Cordycipitaceae): transcriptional analysis and molecular characterization of cox1 and group I intron with putative LAGLIDADG endonuclease

Genomes of Plant-Associated Clavicipitaceae

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