Rhabdophis chiwen
is currently found so far in Sichuan Province, China, where it predominantly feeds on earthworms and firefly larvae. In this study, we sequenced and analyzed the mitochondrial genome of
R. chiwen
, which measured 17,646 bp in length and encompassed 37 genes along with two control regions. The base composition revealed percentages of 33.20% A, 25.94% T, 13.27% G, and 27.59% C. Phylogenetic analyses indicate that
R. chiwen
belongs to the family Colubridae and forms a sister branch with
R. tigrinus
. This study successfully obtained the first complete mitochondrial genome of
R. chiwen
, offering crucial genetic data for its evolutionary history and conservation.