The Complete Mitochondrial Genome of the Longhorn Beetle Dorysthenes paradoxus (Coleoptera: Cerambycidae: Prionini) and the Implication for the Phylogenetic Relationships of the Cerambycidae Species

Abstract

The longhorn beetle Dorysthenes paradoxus (Faldermann, 1833) (Coleoptera: Cerambycidae) is not only a serious agricultural pest but also a traditionally edible insect in China. However, no genetic information on this species has been acquired. In the present study, we report the mitochondrial genome (mitogenome) of Do. paradoxus, as the first complete mitogenome of Prioninae. The circular mitogenome of 15,922 bp encodes 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and two ribosomal RNAs (rRNAs), and it contains an A+T-rich region. This mitogenome exhibits the lowest A+T content (71.13%) but harbors the largest AT skew (0.116) among the completely sequenced Cerambycidae species. Eleven of the 13 PCGs have a typical ATN start codon, whereas COI and ND1 are tentatively designated by AAT and TTG, respectively. Only 4 of the 13 PCGs harbor a complete termination codon, and the remaining 9 possess incomplete termination codons (T or TA). Apart from tRNASer(AGN), the other 21 tRNAs can fold into a typical clover-leaf secondary structures. The Do. paradoxus A+T-rich region contains two poly-T stretches and a tandem repeat that comprises two 47-bp-long copies. Both Bayesian inference and Maximum likelihood analyses confirmed the subfamily ranks of Cerambycidae ([Prioninae + Cerambycinae] + Lamiinae) and the close relationship between Philinae and Prioninae/Cerambycinae. However, the data did not support the monophyly of Prioninae and Cerambycinae. The mitogenome presented here provides basic genetic information for this economically important species.

The complete mitochondrial genome ofXystrocera globosa(Coleoptera: Cerambycidae) and its phylogeny

“…The mitochondrial genome of X. globosa is 15,707 bp in length, containing 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and the A þ T-rich region. The mitogenome arrangement is identical to the typical coleopteran insect format, with the ancestral gene order of tRNA Ile -tRNA Gln -tRNA Met located between the A þ T-rich region and ND2 (Liu et al 2018). The initiation codon of the 12 proteincoding genes excluding ND1 (that uses TTG) is the typical ATN form (ATA, ATG, ATC, ATT), whereas 10 protein-coding genes (excluding ND3, ND4, and ND5 that end with a single T) have termination codons of TAA or TAG.…”

The complete mitochondrial genome ofAnnamanum lunulatum(Coleoptera: Lamiinae) and its phylogeny

“…In order to reconstruct the phylogenetic relationships of Lamiinae, we used 13 protein-coding genes of 24 species, which contained Galeruca daurica as the outgroup (Kim et al 2009;Lu et al 2011;Chiu et al 2016;Fang et al 2016;Guo et al 2016;Li et al 2016;Wang et al 2016;Lim et al 2017;Liu et al 2017;Yang et al 2017;Liu et al 2018;Que et al 2019;Wang, Lan, et al 2019). MrBayes version 3.2 and the RAxML version 8 programs (Ronquist et al 2012;Stamatakis 2014) were used to construct BI and ML trees, respectively (Figure 1).…”

The complete mitochondrial genome of an Asian longicorn beetle Glenea cantor (Coleoptera: Cerambycidae: Lamiinae)

“…Moreover, it has high reproductive ability (Lu et al 2013) and its host range makes it a possible invasive pest outside Asia if established (Lu, et al 2011b). Mitochondrial genome sequences are essential to a deeper understanding of the evolution of Cerambycidae and identify larva species (Liu et al 2018;Wang and Tang 2018). Here, the complete mitochondrial DNA (mtDNA) genome of G. cantor was elucidated which has not been reported before.…”