The complete mitochondrial genome sequence of <i>Chrysanthemum boreale</i> (Asteraceae)

Won, So Youn; Jung, Jae A; Kim, Jung Sun

doi:10.1080/23802359.2018.1468226

Cited by 9 publications

(5 citation statements)

References 12 publications

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“…The lengths of the mitochondrial genomes of these Bidens species were similar to those of most land plants reported in the organelle database of NCBI (Supplementary Figure S9) and was similar to that of Tanacetum vulgare (Won et al, 2018). The total GC content of the mitochondrial genomes of the Bidens species was between 45.4 and 45.8% and were similar to that of Helianthus annuus (Grassa et al, 2016).…”

Section: Discussionsupporting

confidence: 75%

Identification of Medicinal Bidens Plants for Quality Control Based on Organelle Genomes

Nie

Guo

et al. 2022

Front. Pharmacol.

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Bidens plants are annuals or perennials of Asteraceae and usually used as medicinal materials in China. They are difficult to identify by using traditional identification methods because they have similar morphologies and chemical components. Universal DNA barcodes also cannot identify Bidens species effectively. This situation seriously hinders the development of medicinal Bidens plants. Therefore, developing an accurate and effective method for identifying medicinal Bidens plants is urgently needed. The present study aims to use phylogenomic approaches based on organelle genomes to address the confusing relationships of medicinal Bidens plants. Illumina sequencing was used to sequence 12 chloroplast and eight mitochondrial genomes of five species and one variety of Bidens. The complete organelle genomes were assembled, annotated and analysed. Phylogenetic trees were constructed on the basis of the organelle genomes and highly variable regions. The organelle genomes of these Bidens species had a conserved gene content and codon usage. The 12 chloroplast genomes of the Bidens species were 150,489 bp to 151,635 bp in length. The lengths of the eight mitochondrial genomes varied from each other. Bioinformatics analysis revealed the presence of 50–71 simple sequence repeats and 46–181 long repeats in the organelle genomes. By combining the results of mVISTA and nucleotide diversity analyses, seven candidate highly variable regions in the chloroplast genomes were screened for species identification and relationship studies. Comparison with the complete mitochondrial genomes and common protein-coding genes shared by each organelle genome revealed that the complete chloroplast genomes had the highest discriminatory power for Bidens species and thus could be used as a super barcode to authenticate Bidens species accurately. In addition, the screened highly variable region trnS-GGA-rps4 could be also used as a potential specific barcode to identify Bidens species.

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Section: Discussionsupporting

confidence: 75%

Identification of Medicinal Bidens Plants for Quality Control Based on Organelle Genomes

Nie

Guo

et al. 2022

Front. Pharmacol.

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“…Organelles were assembled by extracting Nanopore and Illumina reads that aligned to the available C. boreale chloroplast (Won et al 2018b) and mitochondria (Won et al 2018c) references using Minimap2 v2.17 (Li 2018) and BWA-MEM v0.7.17-r1198-dirty (Li 2013) respectively. A hybrid assembly was then performed for each organelle using Unicycler v0.4.8 (Wick et al 2017).…”

Section: Genome Assembly and Scaffoldingmentioning

confidence: 99%

De novo whole-genome assembly ofChrysanthemum makinoi, a key wild ancestor to hexaploid Chrysanthemum

Lieshout

Kaauwen

Kodde

et al. 2021

Preprint

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Chrysanthemum is among the top ten cut, potted and perennial garden flowers in the world. Despite this, to date, only the genomes of two wild diploid chrysanthemums have been sequenced and assembled. Here we present the most complete and contiguous chrysanthemum de novo assembly published so far, as well as a corresponding ab initio annotation. The wild diploid Chrysanthemum makinoi is thought to be one of the ancestors of the cultivated hexaploid varieties which are currently grown all around the world. Using a combination of Oxford Nanopore long reads, Pacific Biosciences long reads, Illumina short reads, Dovetail sequences and a genetic map, we assembled 3.1 Gb of its sequence into 9 pseudochromosomes, with an N50 of 330 Mb and BUSCO complete score of 92.1%. Our ab initio annotation pipeline predicted 95 074 genes and marked 80.0% of the genome as repetitive. This genome assembly of C. makinoi provides an important step forward in understanding the chrysanthemum genome, evolution and history.

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“…Chrysanthemum ( Chrysanthemum morifolium ) is known as an ornamental plant that has a variety of flower shapes, sizes, and colors (Wang et al, 2015 ; Won et al, 2018 ). The global count of chrysanthemum cultivars has been estimated to exceed 20000 (Wang et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

Setiawati,

Arofah,

Nurzaman

et al. 2023

bta

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Chrysanthemum ( Chrysanthemum morifolium ) contains secondary metabolites, such as flavonoid compounds, especially luteolin-7-glucoside and quercetin-3-O-rhamnoside (quercitrin), in its tissues. Utilizing sucrose as an elicitor through callus culture presents an alternative method to enhance the production of secondary metabolites. This research aimed to determine the best sucrose concentration and harvest time for maximizing quercitrin content in chrysanthemum callus culture. The research employed a completely randomized design with four treatment groups: 0, 30, 45, and 60 g/l of sucrose added to MS medium containing 4 ppm 2,4-dichlorophenoxyacetic acid (2,4-D). Callus samples were harvested on the 15th and 30th days of culture. The observed parameters included callus morphology (color and texture), fresh weight, dry weight, the diameter of the callus, and quercitrin content analyzed using high-performance liquid chromatography. The results showed that all callus cultures exhibited intermediate textures and varied colors, predominantly shades of brown. The treatment involving 45 g/l of sucrose with a 30th-day harvest yielded the highest fresh weight, dry weight, and quercitrin content, namely 2.108 g, 0.051 g, and 0.437 mg/g DW, respectively. Notably, the quercitrin content exhibited a 63.67% increase compared to the control.

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The complete mitochondrial genome sequence of Chrysanthemum boreale (Asteraceae)

Cited by 9 publications

References 12 publications

Identification of Medicinal Bidens Plants for Quality Control Based on Organelle Genomes

Identification of Medicinal Bidens Plants for Quality Control Based on Organelle Genomes

De novo whole-genome assembly ofChrysanthemum makinoi, a key wild ancestor to hexaploid Chrysanthemum

Effect of sucrose as an elicitor in increasing quercetin-3-O-rhamnoside (quercitrin) content of chrysanthemum (Chrysanthemum morifolium Ramat) callus culture based on harvest time differences

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