“…The taxa in the analyses included the sympatric and congeneric species in the region: P. nigromaculatus, P. plancyi, P. chosenicus, P. mongolius, P. hubeiensis, and P. fukienensis; along with five outgroup taxa consist of the western Palearctic P. bedriagae and P. lessonae, and three Asian ranids; Glandirana emeljanovi, G. rugosa, and Amolops granulosus (Table S1). To align the sequences, we first retrieved the sequences for ingroups and outgroups from BLASTn and GenBank (accession numbers in Table S1; originating from Min et al 2008, Zhang et al 2008, Liu et al 2010, Ryu and Hwang 2011, Jeong et al 2013, Komaki et al 2015, Hofman et al 2016, Jiang et al 2017, Eo et al 2019, Huang et al 2019, Tokumoto et al 2019, Oike et al 2020, Margaryan et al 2021, Zhou et al 2022b, and Zhou et al 2022a). We analysed and trimmed the DNA sequences using Geneious Prime version 2022.1 (http://www.geneious.com, Kearse et al 2012), and aligned the sequences using Clustal Omega (Sievers et al 2011) with the default settings in the same Geneious Prime program.…”