The complete nucleotide sequence and genome organisation of a novel member of the family Betaflexiviridae from Actinidia chinensis

Veerakone, S.; Liefting, L. W.; Tang, Joe; Ward, L. I.

doi:10.1007/s00705-017-3701-x

Cited by 22 publications

(20 citation statements)

References 4 publications

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“…The virus (Actinidia chlorotic ringspot-associated virus, AcCRaV), presents double-membrane bodies in infected tissues, and has a genome composed of RNA segments in negative polarity, so it covers the typical features of members in the genus Emaravirus . A final report from New Zealand [ 90 ] described the complete genome sequence of a novel virus, tentatively named Actinidia seed-borne latent virus (ASbLV). The virus was identified from Actinidia chinensis (a seedling in home garden), its genome contains four open reading frames, and is most closely related to CPrV (56% nt identity), a member of the genus Prunevirus in Betaflexiviridae .…”

Section: Application Of High-throughput Sequencing Technology To Dmentioning

confidence: 99%

Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies

Maliogka

Minafra

Saldarelli

et al. 2018

Viruses

108

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Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., Fabavirus, Luteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed.

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Section: Application Of High-throughput Sequencing Technology To Dmentioning

confidence: 99%

Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies

Maliogka

Minafra

Saldarelli

et al. 2018

Viruses

108

View full text Add to dashboard Cite

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“…The first virus naturally infecting kiwifruit was reported in 2003 (Clover et al , 2003) and only three kiwifruit viruses had been reported by 2010 (Clover et al , 2003; Chavan et al , 2009; 2013). Recent studies have shown that at least 17 viruses have been detected in natural infections of kiwifruit, including cucumber necrosis virus (CNV), radish mosaic virus (RMV), cherry leaf roll virus (CLRV), cucumber mosaic virus (CMV), citrus leaf blotch virus (CLBV), potato virus X (PVX), pelargonium zonate spot virus (PZSV), tomato necrotic spot associated virus (TNSaV), tomato zonate spot virus (TZSV), turnip vein clearing virus (TVCV), Actinidia virus A (AcVA), Actinidia virus B (AcVB), Actinidia virus X (AVX), Actinidia virus 1 (AcV‐1), Actinidia seed‐borne latent virus (ASbLV) and Actinidia chlorotic ringspot‐associated emaravirus (AcCRaV) (Pearson et al , 2011; Blouin et al , 2012; 2013; 2018; Chavan et al , 2012; 2013; James and Phelan, 2016; Wang et al , 2016; Zheng et al , 2017; Veerakone et al , 2018; Zhao et al , 2019).…”

Section: Introductionmentioning

confidence: 99%

“…and Actinidia chlorotic ringspot-associated emaravirus (AcCRaV) (Pearson et al, 2011;Blouin et al, 2012;Chavan et al, 2012;James and Phelan, 2016;Wang et al, 2016;Zheng et al, 2017;Veerakone et al, 2018;Zhao et al, 2019).…”

mentioning

confidence: 99%

Occurrence and molecular characterization of Actinidia virus C (AcVC), a novel vitivirus infecting kiwifruit (Actinidia spp.) in China

et al. 2020

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Kiwifruit (Actinidia spp.) is an economically important fruit crop in the world. China is the largest producer of kiwifruit in terms of both acreage and yield. In this study, a novel virus, tentatively named Actinidia virus C (AcVC), was discovered in Actinidia deliciosa ‘Xuxiang’ grown in commercial kiwifruit fields in the Shaanxi province, a major kiwifruit‐growing region in China. Sequencing of the complete genome showed that AcVC has 7,668 nucleotides (not including the poly‐(A) sequence). Complete genome comparison and phylogenetic analysis showed that AcVC is most closely related to AcVA and then to AcVB. AcVC shares 69.2% nucleotide identity with AcVA (GenBank accession number ). Based on these data, Actinidia virus C is assigned as a new tentative member of the genus Vitivirus in the family Betaflexiviridae. AcVC was found to infect A. deliciosa and A. chinesis, the two major cultivated kiwifruits. Infection frequency of AcVC varied with the kiwifruit‐growing regions and cultivars in the Shaanxi province of China.

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“…In 2003, New Zealand researchers discovered for the first time the symptoms of viral infection caused by Apple stem grooving virus in a number of kiwifruit seedlings imported from China [3]. Subsequently, sixteen kiwifruit viruses have been identified worldwide, including eight that are non-specific: Apple stem grooving virus, Actinidia virus X, Alfalfa mosaic virus, Cucumber necrosis virus, Cucumber mosaic virus, Ribgrass mosaic virus, Turnip vein clearing virus and Tomato necrotic spot associated virus [3][4][5][6], six specific kiwifruit viruses: Actinidia virus A, Actinidia virus B, Actinidia citrivirus, Actinidia virus 1, Actinidia chlorotic ringspot-associated virus and Actinidia seed-borne latent virus [7][8][9][10], and two pathogenic viruses (Cherry leaf roll virus and Pelargonium zonate spot virus) [4]. These known kiwifruit viruses belong to different families, including Betaflexiviridae, Bromoviridae, Virgaviridae, Tombusviridae, Bunyaviridae, Secoviridae, and Closteroviridae.…”

Section: Introductionmentioning

confidence: 99%

Characterization and complete genome sequences of two novel variants of the family Closteroviridae from Chinese kiwifruit

et al. 2020

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Two distinct closterovirus-like genome sequences (termed AdV-1 v1 and v2) were identified in Actinidia chinensis var. deliciosa ‘Miliang-1’ that had no disease symptoms using high-throughput sequencing. Using overlapping reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends, the genomic sequences of AdV-1 v1 and v2 were confirmed as 17,646 and 18,578 nucleotides in length, respectively. The two complete genomes contained 9 and 15 open reading frames, respectively, coding for proteins having domains typical of Closteroviridae, such as RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homolog (HSP70h) and coat protein (CP). Sequence analysis showed that the amino acid sequences of RdRp, HSP70h, and CP of the two variants exhibited high similarity (> 80%), while their genomic organization was somewhat different. This suggested that the two viral genomes identified here are variants of the family Closteroviridae in a single kiwifruit host. Furthermore, phylogenetic relationship analysis revealed that the two variants had a closer relationship with the unclassified virus Persimmon virus B (PeVB) and Actinidia virus 1 (AcV-1) than with other members of the family Closteroviridae, as did their genomic organization. It is speculated that the two variants, together with PeVB and AcV-1 belong to a new subfamily of Closteroviridae.

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The complete nucleotide sequence and genome organisation of a novel member of the family Betaflexiviridae from Actinidia chinensis

Cited by 22 publications

References 4 publications

Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies

Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies

Occurrence and molecular characterization of Actinidia virus C (AcVC), a novel vitivirus infecting kiwifruit (Actinidia spp.) in China

Characterization and complete genome sequences of two novel variants of the family Closteroviridae from Chinese kiwifruit

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