The complete nucleotide sequence of plum pox virus RNA (strain D)

Nucleotide sequence of the 3'-terminal region of the RNA of the E1 Amar strain of plum pox potyvirus

“…These results raise the question of the origin of PPV-E1 Amar and of other widely divergent strains. Since the spread of sharka disease from Eastern Europe to Western Europe and the Mediterranean region over the past 50 years is one of the best documented cases of extension of a plant virus Multiple alignment, generated using the CLUSTAL program (Higgins & Sharp, 1988), of coat protein sequences of four different PPV strains, PPV-E1 Amar, PPV-D (Teycheney et al, 1989), PPV-Rankovik (Lain et aL, 1989) and PPV-NAT (Maiss et al, 1989). Conserved amino acids (found in at least two of the four sequences) are shaded.…”

“…CC~T~.1q/q*q~G~TCGIvG~kT~IvC~qIv1q*q`TC~IvC/q~0CTP~CCCJ~GG~TTTTCCCCCATTCC~C~A~&~CT~TC~IvT~T~~T~T~ Teycheney et al, 1989), revealed a level of similiarity of 81%; identical levels of similarity were seen between PPV-EI Amar and the corresponding sequences of the two other strains, PPV-NAT (Maiss et al, 1989) and PPV-Rankovik (Lain et al, 1989), sequenced previously. In contrast, strains D, NAT and Rankovik are up to 98 identical.…”

“…Comparisons at the amino acid level revealed 91 ~o similarity between the 3' half of PPV-EI Amar polyprotein and the corresponding sequence of PPV-D, indicating that many of the nucleotide changes observed are silent; comparable levels of similarity were seen between PPV-EI Amar and the other sequenced strains of PPV. All predicted polyprotein cleavage sites (Garcia et al, 1989;Martin et al, 1990) of PPV-E1Amar were found to be the same as those in PPV-D (Teycheney et al, 1989) or PPV-Rankovik (Lain et al, 1989, except for two amino acid changes in the NIa/NIb cleavage site, where the sequence reads NVVVH(Q/A) instead of TVVVH(Q/S), the recognition sequence for other strains. However, these modifications are not predicted to affect the cleavage of the polyprotein significantly (Dougherty et al, 1989).…”

“…This sequence was then compared to the corresponding sequence of previously sequenced PPV strains, PPV-D (Teycheney et al, 1989), PPV-NAT (Maiss et al, 1989) and PPV-Rankovik (Lain et al, 1989).…”

Sequence polymorphism in the 5'NTR and in the P1 coding region of potato virus Y genomic RNA

“…The flexuous particles contain a single-stranded messenger RNA of about 10000 nucleotides with a viral protein covalently linked to the 5' end and a poly(A) tail at the 3' end (for a review see Riechmann et al, 1992). The complete nucleotide sequence of several members of the group is already known (Allison et al, 1986;Domier et al, 1986;Robaglia et al, 1989;Lain et al, 1989;Maiss et al, 1989;Teycheney et al, 1989;Johansen et al, 1991 ; * Author for correspondence. Fax +33 1 30 83 31 95. e-mail robaglia@oxygene.versailles.inra.fr Nicolas & Lalibertr, 1992;Yeh et al, 1992;Jayaram et al, 1992;Vance et al, 1992;Thole et al, 1993;Palkovics et al, 1993).…”