The complete plastome of <i>Cynanchum rostellatum</i> (Apocynaceae), an indigenous plant in Korea

Lee, Sae Hyun; Jang, Woojong; Kim, Eunbi; Kim, Jiseok; Gong, Haiguang; Kang, Jae Soon; Shim, Hyeonah; Park, Jee Young; Yang, Tae-Jin

doi:10.1080/23802359.2022.2148489

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…All 8 species and varieties of the ingroup, except C. komarovii , comprise a group that supports the monophyly of the Cynanchum clade, This phylogenetic result was similar to those of Lee et al. ( 2022 ) and Pei et al. ( 2022 ).…”

Section: Discussionsupporting

confidence: 88%

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Characterization of the complete chloroplast genome of Cynanchum acutum subsp. s ibiricum (Apocynaceae)

Zhang,

Liu,

Wang

et al. 2023

Mitochondrial DNA Part B

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In this study, we assembled the complete chloroplast (cp) genome of Cynanchum acutum subsp. sibiricum using high-throughput Illumina sequencing reads. The resulting chloroplast genome assembly displayed a typical quadripartite structure with a total length of 158,283 bp, which contained a pair of inverted repeat regions (IRs) of 24,459 bp. These two IRs were separated by a large single-copy region (LSC) and a small single-copy region (SSC) of 89,424 bp and 19,941 bp in length, respectively. The C. acutum subsp. sibiricum cp genome contained 130 genes, and its overall GC content was 37.87%. Phylogenetic analysis among C. acutum subsp. sibiricum and nine other Cynanchum species demonstrated that C. acutum subsp. sibiricum was closely related to C. chinense . The C. acutum subsp. sibiricum cp genome presented in this study lays a good foundation for further genetic and genomic studies of the Cynanchum as well as Apocynaceae.

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Section: Discussionsupporting

confidence: 88%

“… 2021 ), Cynanchum rostellatum OQ852689 (Lee et al. 2022 ), Cynanchum wilfordii KX352467 (Hyun-Seung, 2016 ) and Nerium oleander KJ953907.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the complete chloroplast genome of Cynanchum acutum subsp. s ibiricum (Apocynaceae)

Zhang,

Liu,

Wang

et al. 2023

Mitochondrial DNA Part B

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Comparative Analysis and Phylogeny of the Complete Chloroplast Genomes of Nine Cynanchum (Apocynaceae) Species

Zhang,

Ma,

Guo

et al. 2024

Genes

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Cynanchum belongs to the Apocynaceae family and is a morphologically diverse genus that includes around 200 shrub or perennial herb species. Despite the utilization of CPGs, few molecular phylogenetic studies have endeavored to elucidate infrafamilial relationships within Cynanchum through extensive taxon sampling. In this research, we constructed a phylogeny and estimated divergence time based on the chloroplast genomes (CPGs) of nine Cynanchum species. We sequenced and annotated nine chloroplast (CP) genomes in this study. The comparative analysis of these genomes from these Cynanchum species revealed a typical quadripartite structure, with a total sequence length ranging from 158,283 to 161,241 base pairs (bp). The CP genome (CPG) was highly conserved and moderately differentiated. Through annotation, we identified a total of 129–132 genes. Analysis of the boundaries of inverted repeat (IR) regions showed consistent positioning: the rps19 gene was located in the IRb region, varying from 46 to 50 bp. IRb/SSC junctions were located between the trnN and ndhF genes. We did not detect major expansions or contractions in the IR region or rearrangements or insertions in the CPGs of the nine Cynanchum species. The results of SSR analysis revealed a variation in the number of SSRs, ranging from 112 to 150. In five types of SSRs, the largest number was mononucleotide repeats, and the smallest number was hexanucleotide repeats. The number of long repeats in the cp genomes of nine Cynanchum species was from 35 to 80. In nine species of Cynanchum, the GC3s values ranged from 26.80% to 27.00%, indicating a strong bias towards A/U-ending codons. Comparative analyses revealed four hotspot regions in the CPG, ndhA-ndhH, trnI-GAU-rrn16, psbI-trnS-GCU, and rps7-ndhB, which could potentially serve as molecular markers. In addition, phylogenetic tree construction based on the CPG indicated that the nine Cynanchum species formed a monophyletic group. Molecular dating suggested that Cynanchum diverged from its sister genus approximately 18.87 million years ago (Mya) and species diversification within the Cynanchum species primarily occurred during the recent Miocene epoch. The divergence time estimation presented in this study will facilitate future research on Cynanchum, aid in species differentiation, and facilitate diverse investigations into this economically and ecologically important genus.

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The complete plastome of Cynanchum rostellatum (Apocynaceae), an indigenous plant in Korea

Cited by 2 publications

References 25 publications

Characterization of the complete chloroplast genome of Cynanchum acutum subsp. s ibiricum (Apocynaceae)

Characterization of the complete chloroplast genome of Cynanchum acutum subsp. s ibiricum (Apocynaceae)

Comparative Analysis and Phylogeny of the Complete Chloroplast Genomes of Nine Cynanchum (Apocynaceae) Species

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