The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene

Abstract

ISSN: (Print) 2380-2359 (Online) Journal homepage: https://www.tandfonline.com/loi/tmdn20The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene The complete plastome of the coccoid green alga Jenufaminuta (Chlorophyceae, incertaesedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene, Mitochondrial DNA Part B, 5:2, 1728-1730,The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene ABSTRACT We describe the plastome of Jenufa minuta, a unicellular green alga whose precise relationship with the Sphaeropleales (Chlorophyceae) remains uncertain. This 206,680-bp genome contains 100 conserved genes, 11 of which exhibit 16 group I and seven group II introns. We discovered a trans-spliced group II intron in the rpl32 gene of J. minuta and its orthologs in all Sphaeropleales and several Chlamydomonadales; this is the first time this intron is documented. As in the mitochondria of J. minuta, the UGA codon is decoded as tryptophan. To our knowledge, this is the second report of a variant genetic code in plastids. ARTICLE HISTORY

Taxonomic reinvestigation of the genus Tetradesmus (Scenedesmaceae; Sphaeropleales) based on morphological characteristics and chloroplast genomes

“…The amino acid sequences of rpl32 are highly conserved in the Tetradesmus species (Figure 1B). The discontinuous structure of rpl32 has been previously reported in Jenufa minuta Nemcova, M.Eliaś, Skaloud & Neustupa (Chlorophyceae, incertae sedis; Turmel et al, 2020). We postulate that diverse exon structures of psaA and rpl32 in the genus Tetradesmus could be derived from species-specific (or infraspecific) genetic variations.…”