The complex effect of temperature on protein degradation in sporulating Bacillus megaterium

Chaloupka, J

doi:10.1016/0378-1097(80)90090-7

Cited by 2 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Netropsin did not affect protein turnover during the first two hours in the sporulation medium. However, the development of a degradative system, involved in the increased rate of protein turnover during later stages of sporogenesis [14] was either decreased (with 0.5 #g/ml) or suppressed (with 1.0 #g/ml) by netropsin ( Fig. 2A).…”

Section: Resultsmentioning

confidence: 99%

Netropsin stimulates the formation of an extracellular proteinase and suppresses protein turnover in sporulating Bacillus megaterium

KUEROVA¹

1986

FEMS Microbiology Letters

View full text Add to dashboard Cite

Netropsin stimulated the rate of synthesis of an extracellular metalloproteinase in Bacillus megaterium incubated in a sporulation medium. The antibiotic delayed but did not suppress the decrease in the ability to synthesize the proteinase occurring at later sporulation stages. Netropsin also stimulated the synthesis of the proteinase when added to a growing culture; it inhibited the increase of protein turnover which was switched on between the 2nd and 3rd hour in the sporulating population. No refractile spores were developed during 6 h at 35°C in the antibiotic-treated culture. In the control 60% of sporulating cells were observed under similar conditions. INTRODUCTIONThe peptidic antibiotic netropsin inhibits sporulation in Bacillus subtilis at concentrations exerting no or negligible effect on growth [1]. The antibiotic seems to suppress the sporogenesis be-* To whom correspondence and reprint requests should be addressed.fore or at the beginning of stage II [2]. Netropsin interacts with the A + T-rich sequences of DNA but it is not intercalated into the DNA molecule as is the case with actinomycin D [3,4]. Netropsin binds within the minor groove of DNA by displacing the water molecules [5]. The antibiotic was found not to affect the synthesis of several enzymes considered to be related to the sporogenesis in B. subtilis (e.g. glucose dehydrogenase and alkaline phosphatase) and it stimulated slightly the formation of the aconitase. However, the excretion of proteinases was decreased and the formation of the coat protein and of the dipicolinic acid characteristic for the later sporulation phases, was suppressed [1,6,7]. The activity of a sporulationspecific form of the RNA polymerase was also decreased by netropsin [8]. B. megaterium synthesizes an extracellular metalloproteinase which is formed during growth as well as during sporulation [9], unlike the metalloand serine proteinases of B. subtilis. The formation of proteinase during growth of B. megaterium is controlled by repression with amino acids, the carbon and energy sources having only a slight effect. However, its synthesis during sporulation seems to be partially under developmental control [101.

show abstract

Section: Resultsmentioning

confidence: 99%

Netropsin stimulates the formation of an extracellular proteinase and suppresses protein turnover in sporulating Bacillus megaterium

KUEROVA¹

1986

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…Also in Bacillus megaterium, an increased temperature stimulated the initial rate of protein degradation during growth and sporogenesis. However, a slightly supraoptimal temperature may suppress the formation of an intracellular proteolytic system participating in sporulation (CHALOUPKA and STRNADOV~ , CHALOUPKA et al 1980). I n Neurospora, no effect of temperature on protein turnover was found in a growing mycelium, whereas a substantial stimulation was observed during starvation (MARTEGANI and ALBBRGHI-NA 1979).…”

mentioning

confidence: 99%

Effect of temperature on growth and protein turnover in Bacillus megaterium

Strnadová

Prasad

Kučerová

et al. 1986

J. Basic Microbiol.

View full text Add to dashboard Cite

The ARRHENIUS plot of the specific growth rate p of an asporogenic mutant of Bacillus nlegaterium, growing in the range of 17-37 "C followed a straight line, whose slope was affected by the composition of the medium. The maximal p was found a t 40 "C in all media tested and decreased rapidly above 42 "C. The portion of protein degraded also followed a straight line but reached its maximal value a t 45-47 "C. I t s slope was affected substantially by the composition of the medium; the mixture of amino acids suppressed protein degradation a t temperature lower than 37 "C. The extent of the short-lived protein fraction, degraded with the half-time of about 1 h, was mostly affected by both -the temperature and the medium. The amount of proteins degraded per one biomass doubling was almost constant up to 37 "C but was affected by the composition of the medium. At higher temperatures it increased rapidly and reached values of 20-40% of pulselabelled proteins degraded per one cell cycle. The degradation of ethionine-containing proteins was similarly dependent on the temperature but the extent of proteins degraded was increased.The effect of temperature on protein synthesis and growth is inteiisively studied a t present, because temperature is considered to be an important factor regulating various biological processes (SCHLESINGER et al. 1982 (RATKOWSKY et al. 1982 and1983) tried to overcome this problem by using the square root plot.Within a narrow range around the optimal temperature (Topt, i. e. the temperature of the maximal value of p), the ARRHENIUS plot of p usually deviates from the straight line. One of the factors responsible for this deviation could be an increased protein turnover, which could result in a disharmony between protein synthesis and degradation.The effect of temperature on protein turnover in Escherichia coli was first studied by PINE (1967, 1973). He found that an increased temperature stimulated protein turnover and that the estent of protein degradation in growing and starving populations was similar a t supraoptimal temperatures. The extensive protein turnover at high temperatures might be explained by a recent observation that one of the intracellular proteinases is a heat-shock protein (PHILLIPS et al. 1984). Also in Bacillus megaterium, an increased temperature stimulated the initial rate of protein degradation during growth and sporogenesis. However, a slightly supraoptimal temperature may I ) Participant of the UNESCO Course on Modern Problems in Biology and Biotechnology.

show abstract

The complex effect of temperature on protein degradation in sporulating Bacillus megaterium

Cited by 2 publications

References 5 publications

Netropsin stimulates the formation of an extracellular proteinase and suppresses protein turnover in sporulating Bacillus megaterium

Netropsin stimulates the formation of an extracellular proteinase and suppresses protein turnover in sporulating Bacillus megaterium

Effect of temperature on growth and protein turnover in Bacillus megaterium

Contact Info

Product

Resources

About