The Complex Story of Plant Cyclic Nucleotide-Gated Channels

Jarratt-Barnham, Edwin; Wang, Limin; Ning, Youzheng; Davies, Julia M.

doi:10.3390/ijms22020874

Cited by 75 publications

(70 citation statements)

References 142 publications

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“…CNGCs can be assembled as homotetrameric or heterotetrameric complexes, allowing for the formation of a large array of sensors capable of responding to different intensities of environmental cues ( Clough et al, 2000 ; Tan et al, 2020 ). The cytosolic C-terminus harbors a cyclic nucleotide-binding domain (CNBD) and a calmodulin-binding domain (CaMBD), in which a calmodulin (CaM) binding isoleucine-glutamine (IQ) motif is embedded ( Kaplan et al, 2007 ; Jarratt-Barnham et al, 2021 ). A. thaliana AtCNGC2 and four acts as a heat sensor since its lack of expression leads to a hyper thermosensitive phenotype, resulting in a higher accumulation of HSPs at lower temperature.…”

Section: Heat Sensing and Signaling In Land Plantsmentioning

confidence: 99%

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

Bourgine

Guihur

2021

Front. Plant Sci.

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Heat stress events are major factors limiting crop productivity. During summer days, land plants must anticipate in a timely manner upcoming mild and severe temperature. They respond by accumulating protective heat-shock proteins (HSPs), conferring acquired thermotolerance. All organisms synthetize HSPs; many of which are members of the conserved chaperones families. This review describes recent advances in plant temperature sensing, signaling, and response. We highlight the pathway from heat perception by the plasma membrane through calcium channels, such as cyclic nucleotide-gated channels, to the activation of the heat-shock transcription factors (HSFs). An unclear cellular signal activates HSFs, which act as essential regulators. In particular, the HSFA subfamily can bind heat shock elements in HSP promoters and could mediate the dissociation of bound histones, leading to HSPs transcription. Although plants can modulate their transcriptome, proteome, and metabolome to protect the cellular machinery, HSP chaperones prevent, use, and revert the formation of misfolded proteins, thereby avoiding heat-induced cell death. Remarkably, the HSP20 family is mostly tightly repressed at low temperature, suggesting that a costly mechanism can become detrimental under unnecessary conditions. Here, the role of HSP20s in response to HS and their possible deleterious expression at non-HS temperatures is discussed.

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Section: Heat Sensing and Signaling In Land Plantsmentioning

confidence: 99%

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

Bourgine

Guihur

2021

Front. Plant Sci.

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“…In contrast to CaM7-arrested CNGC14, the activity of other CNGCs could be positively regulated by CaM binding (e.g. CaM1 to CNGC12) (Dietrich, Moeder, & Yoshioka, 2020;Jarratt-Barnham, Wang, Ning, & Davies, 2021). These aforementioned studies have shed light on the diverse regulatory mechanism of CNGC.…”

Section: Discussionmentioning

confidence: 99%

A novel Calmodulin-interacting Domain of Unknown Function 506 protein represses root hair elongation in Arabidopsis

Ying

Scheible

2021

Preprint

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DUF506 proteins are omnipresent in higher plants. Phosphorus (P) stress-inducible AtRXR1/REPRESSOR OF EXCESSIVE ROOT HAIR ELONGATION 1 gene, as the first functionally characterized DUF506 gene, is proved to inhibit root hair elongation through interaction of RXR2/RabD2c GTPase. However, the knowledge of other P-responsive DUF506 genes is still limited. Here, we identify four additional P-inducible DUF506 genes and select two of candidates for functional investigation. Expression analysis results reveal that both of candidates are induced by auxin. At3g07350, the duplicated pair of RXR1, expresses ubiquitously in seedlings under P-stress, whereas At1g62420 is mainly induced in roots. Overexpression and knockout mutants of At1g62420, which is called RXR3, exhibit shorter or longer root hair length, respectively. Cellular imaging results demonstrate RXR3 localizes in root epidermal cells. ChIP, synthetic peptide treatment and qRT-PCR assay results indicate RXR3 is transcriptionally activated by RSL4 or RALF1. BiFC and CaM-binding assay suggest that RXR3 interacts with various CaMs in presence of Ca2+. Moreover, the frequencies of [Ca2+]cyt in rxr3 mutants are approximately 20% higher compared to that of wild type. Taken together, our results illustrate a divergent mechanism by which RSL4-directed RXR3 interacts with calmodulin and functions in parallel of RXR1, to prevent root hair excessive growth.

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“…CIPK proteins are activated by calcineurin B-like proteins (CBLs) and phosphorylated loop region located in the kinase domain ( Tang et al, 2020 ), and are involved in the salinity tolerance mechanisms ( D’Angelo et al, 2006 ). The members of the CNGC gene family were reported to undergo phosphorylation and post-translational modifications ( Kakar et al, 2017 ), and the phosphorylation of CNGC was believed to be responsible for defense signaling ( Jarratt-Barnham et al, 2021 ). Therefore, we speculated that LZ04 attenuates the effects of high calcium stress on A. thaliana , and related genes may be involved in this process by the regulation of calcium-dependent phosphorylation systems.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of Calcium Metabolism Related Gene Families in Arabidopsis thaliana and Their Regulation by Bacillus amyloliquefaciens Under High Calcium Stress

et al. 2021

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Several gene families involved in calcium signaling have been detected in plants, including calmodulin (CaM), calcium dependent protein kinases (CDPK), calcineurin B-like (CBL) and cyclic nucleotide-gated channels (CNGCs). In our previous study, we demonstrated that Bacillus amyloliquefaciens LZ04 (B. amyloliquefaciens LZ04) regulate genes involved in calcium stress in Arabidopsis thaliana (A. thaliana). Here, we aimed to explore the potential involvement of calcium-related gene families in the response of A. thaliana to calcium stress and the potential regulatory effects of B. amyloliquefaciens LZ04 on these genes. The structure, duplication, synteny, and expression profiles of 102 genes in calcium-related gene families in A. thaliana were investigated. Hidden Markov Models (HMMs) and BLASTP were used to predict candidate genes and conserved domains of the candidate genes were confirmed in SMART and NCBI CDD databases. Gene duplications and synteny were uncovered by BLASTP and phylogenetic analysis. The transcriptome expression profiles of candidate genes were investigated by strand-specific sequencing. Cluster analysis was used to find the expression profiles of calcium-related genes families under different treatment conditions. A total of 102 genes in calcium-related gene families were detected in A. thaliana genome, including 34 CDPK genes, 20 CNGC genes, 18 CIPK genes, 22 IQD genes, and 10 CBP genes. Additionally, of the 102 genes, 33 duplications (32.35%) and 26 gene pairs including 48 genes (47.06%) were detected. Treatment with B. amyloliquefaciens LZ04 enhanced the resistance of A. thaliana under high calcium stress by regulating some of the genes in the calcium-related gene families. Functional enrichment analysis revealed that the genes clustered in the 42nd expression profile which may be B. amyloliquefaciens-responsive genes under calcium stress were enriched in protein phosphorylation and protein modification process. Transcriptome data was validated by RT-PCR and the results generally corroborated the transcriptome sequencing results. These results may be useful for agricultural improvement in high calcium stress regions.

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The Complex Story of Plant Cyclic Nucleotide-Gated Channels

Cited by 75 publications

References 142 publications

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

A novel Calmodulin-interacting Domain of Unknown Function 506 protein represses root hair elongation in Arabidopsis

Genome-Wide Identification and Characterization of Calcium Metabolism Related Gene Families in Arabidopsis thaliana and Their Regulation by Bacillus amyloliquefaciens Under High Calcium Stress

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