The complexity of mesoporous silica nanomaterials unravelled by single molecule microscopy

Lebold, Timo; Michaelis, Jens; Bräuchle, Christoph

doi:10.1039/c0cp02210a

Cited by 35 publications

(37 citation statements)

References 81 publications

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“…Overall, we envision that fcsSOFI could be applied to a diverse class of porous materials, including synthetic soft polymers such as hydrogels, 1 phase separated block 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 copolymers, 11 and polymers, 6, 10 biological environments such as the cellular cytosol 3 and membrane, 4, 5 and heterogeneous hard porous materials such as surfactant-filled mesoporous silica, 11,47 zeolites, 2 metal-organic-frameworks, 7 and activated carbon. 8 In the latter inorganic systems with dense pore networks, the rate of diffusion and spatial alignment of pores would be expected to be able to be resolved by fcsSOFI, but a possible limitation would be resolving every single pore due to the current resolution enhancement of a factor of ~ < 2.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging

Kisley¹,

Brunetti

Tauzin³

et al. 2015

ACS Nano

109

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Porous materials such as cellular cytosol, hydrogels, and block copolymers have nanoscale features that determine macroscale properties. Characterizing the structure of nanopores is difficult with current techniques due to imaging, sample preparation, and computational challenges. We produce a super-resolution optical image that simultaneously characterizes the nanometer dimensions of and diffusion dynamics within porous structures by correlating stochastic fluctuations from diffusing fluorescent probes in the pores of the sample, dubbed here as "fluorescence correlation spectroscopy super-resolution optical fluctuation imaging" or "fcsSOFI". Simulations demonstrate that structural features and diffusion properties can be accurately obtained at sub-diffraction-limited resolution. We apply our technique to image agarose hydrogels and aqueous lyotropic liquid crystal gels. The heterogeneous pore resolution is improved by up to a factor of 2, and diffusion coefficients are accurately obtained through our method compared to diffraction-limited fluorescence imaging and single-particle tracking. Moreover, fcsSOFI allows for rapid and high-throughput characterization of porous materials. fcsSOFI could be applied to soft porous environments such hydrogels, polymers, and membranes in addition to hard materials such as zeolites and mesoporous silica.

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Section: Resultsmentioning

confidence: 99%

Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging

Kisley¹,

Brunetti

Tauzin³

et al. 2015

ACS Nano

109

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“…Single molecule fluorescence microscopy and tracking can be used to obtain valuable information about the porous system and the host‐guest interactions of nanomaterials . The dynamics of guests inside porous nanoparticles, and the interactions of the guests with the walls of the nanoparticles are sometimes the key for the targeted application.…”

Section: Characterization Methodsmentioning

confidence: 99%

Nanoparticle Characterization: What to Measure?

et al. 2019

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What to measure? is a key question in nanoscience, and it is not straightforward to address as different physicochemical properties define a nanoparticle sample. Most prominent among these properties are size, shape, surface charge, and porosity. Today researchers have an unprecedented variety of measurement techniques at their disposal to assign precise numerical values to those parameters. However, methods based on different physical principles probe different aspects, not only of the particles themselves, but also of their preparation history and their environment at the time of measurement. Understanding these connections can be of great value for interpreting characterization results and ultimately controlling the nanoparticle structure–function relationship. Here, the current techniques that enable the precise measurement of these fundamental nanoparticle properties are presented and their practical advantages and disadvantages are discussed. Some recommendations of how the physicochemical parameters of nanoparticles should be investigated and how to fully characterize these properties in different environments according to the intended nanoparticle use are proposed. The intention is to improve comparability of nanoparticle properties and performance to ensure the successful transfer of scientific knowledge to industrial real‐world applications.

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“…These methods are now being applied in studies of mass transport within nanostructured materials (23)(24)(25)(26). Because single-molecule measurements avoid the signal averaging of ensemble methods, it is possible, in principle, to distinguish between the individual processes depicted in Figure 1 in one experiment, while simultaneously quantifying the characteristic temporal and distance scales over which they occur.…”

Section: Single-molecule Methods For Mass Transport Studiesmentioning

confidence: 99%

Single-Molecule Investigations of Morphology and Mass Transport Dynamics in Nanostructured Materials

Higgins

Park

Tran-Ba

et al. 2015

Annual Rev. Anal. Chem.

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Nanostructured materials such as mesoporous metal oxides and phase-separated block copolymers form the basis for new monolith, membrane, and thin film technologies having applications in energy storage, chemical catalysis, and separations. Mass transport plays an integral role in governing the application-specific performance characteristics of many such materials. The majority of methods employed in their characterization provide only ensemble data, often masking the nanoscale, molecular-level details of materials morphology and mass transport. Single-molecule fluorescence methods offer direct routes to probing these characteristics on a single-molecule/single-nanostructure basis. This article provides a review of single-molecule studies focused on measurements of anisotropic diffusion, adsorption, partitioning, and confinement in nanostructured materials. Experimental methods covered include confocal and wide-field fluorescence microscopy. The results obtained promise to deepen our understanding of mass transport mechanisms in nanostructures, thus aiding in the realization of advanced materials systems.

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The complexity of mesoporous silica nanomaterials unravelled by single molecule microscopy

Cited by 35 publications

References 81 publications

Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging

Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging

Nanoparticle Characterization: What to Measure?

Single-Molecule Investigations of Morphology and Mass Transport Dynamics in Nanostructured Materials

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