The composition of house dust mite is critical for mucosal barrier dysfunction and allergic sensitisation

Post, S.; Nawijn, Martijn C.; Hackett, Tillie L.; Baranowska, M.; Gras, R.; Oosterhout, Antoon J. M. van; Heijink, Irene H.

doi:10.1136/thoraxjnl-2011-200606

Cited by 137 publications

(159 citation statements)

References 37 publications

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“…3E), in line with previous observations by others (25). Furthermore, cigarette smoke exposure increased mRNA expression of CCL20, which previously was reported to be induced in airway epithelial cells upon disruption of the airway epithelial barrier integrity (32). In line with mRNA expression, we also detected cigarette smoke-induced IL-8 protein secretion in the basal medium and apical surface liquid (Fig.…”

Section: Nthi Increases Expression Of Rnase 7 In Undifferentiated S-psupporting

confidence: 92%

Basal Cells Contribute to Innate Immunity of the Airway Epithelium through Production of the Antimicrobial Protein RNase 7

Amatngalim

Wijck

Mooij-Eijk

et al. 2015

The Journal of Immunology

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Basal cells play a critical role in the response of the airway epithelium to injury and are recently recognized to also contribute to epithelial immunity. Antimicrobial proteins and peptides are essential effector molecules in this airway epithelial innate immunity. However, little is known about the specific role of basal cells in antimicrobial protein and peptide production and about the regulation of the ubiquitous antimicrobial protein RNase 7. In this study, we report that basal cells are the principal cell type producing RNase 7 in cultured primary bronchial epithelial cells (PBEC). Exposure of submerged cultured PBEC (primarily consisting of basal cells) to the respiratory pathogen nontypeable Haemophilus influenzae resulted in a marked increase in expression of RNase 7, although this was not observed in differentiated air–liquid interface cultured PBEC. However, transient epithelial injury in air–liquid interface–cultured PBEC induced by cigarette smoke exposure led to epidermal growth factor receptor–mediated expression of RNase 7 in remaining basal cells. The selective induction of RNase 7 in basal cells by cigarette smoke was demonstrated using confocal microscopy and by examining isolated luminal and basal cell fractions. Taken together, these findings demonstrate a phenotype-specific innate immune activity of airway epithelial basal cells, which serves as a second line of airway epithelial defense that is induced by airway epithelial injury.

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Section: Nthi Increases Expression Of Rnase 7 In Undifferentiated S-psupporting

confidence: 92%

Basal Cells Contribute to Innate Immunity of the Airway Epithelium through Production of the Antimicrobial Protein RNase 7

Amatngalim

Wijck

Mooij-Eijk

et al. 2015

The Journal of Immunology

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“…To test whether SCIT also affected the innate response to allergens, we assessed levels of pro‐inflammatory chemokines and alarmins released by lung structural cells upon HDM exposure 7. Here, we observe that challenges clearly induced release of eotaxin, IL‐33, TARC/CCL17, and MIP3a/CCL20 indicating activation of the innate immune system and structural cells (Figure 5B,C).…”

Section: Resultsmentioning

confidence: 94%

“…Before and after SCIT treatment, an ear swelling test (EST) was performed to evaluate the early‐phase response to HDM to test for allergic sensitization, as previously described 7, 19…”

Section: Methodsmentioning

confidence: 99%

“…House dust mite (HDM)‐spIgE, Der p1‐spIgE and p2‐spIgE, Der p1‐spIgG1, and Der p1‐spIgG2a levels were measured by ELISA in a similar protocol as described previously7 and in Table S1.…”

Section: Methodsmentioning

confidence: 99%

“…Crude extracts contain the full array of major allergens that a patient can be sensitized to, which increases likelihood of therapeutic efficacy in patients without the need of component‐resolved diagnosis prior to treatment. Additionally, crude extracts also contain numerous nonprotein constituents such as chitins, β‐glucans, and endotoxins, all of which can act on innate immune cells in a pro‐inflammatory fashion, which might interfere with the tolerance‐inducing capacity required for successful therapy 7. In contrast, use of purified proteins allows for treatment with specific allergens in the absence of such components contained within extracts 8.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Subcutaneous immunotherapy with purified Der p1 and 2 suppresses type 2 immunity in a murine asthma model

Hesse

Ieperen

Habraken

et al. 2018

Allergy

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BackgroundAllergen‐specific immunotherapy can induce long‐term suppression of allergic symptoms, reduce medication use, and prevent exacerbations of allergic rhinitis and asthma. Current treatment is based on crude allergen extracts, which contain immunostimulatory components such as β‐glucans, chitins, and endotoxin. Use of purified or recombinant allergens might therefore increase efficacy of treatment.AimsHere, we test application of purified natural group 1 and 2 allergens from Dermatophagoides pteronyssinus (Der p) for subcutaneous immunotherapy (SCIT) treatment in a house dust mite (HDM)‐driven mouse model of allergic asthma.Materials and methods HDM‐sensitized mice received SCIT with crude HDM extract, a mixture of purified Der p1 and 2 (DerP1/2), or placebo. Upon challenges, we measured specific immunoglobulin responses, allergen‐induced ear swelling response (ESR), airway hyperresponsiveness (AHR), and inflammation in bronchoalveolar lavage fluid (BAL) and lung tissue.Results ESR measurement shows suppression of early allergic response in HDM‐SCIT– and DerP1/2‐SCIT–treated mice. Both HDM‐SCIT and DerP1/2‐SCIT are able to suppress AHR and eosinophilic inflammation. In contrast, only DerP1/2‐SCIT is able to significantly suppress type 2 cytokines in lung tissue and BAL fluid. Moreover, DerP1/2‐SCIT treatment is uniquely able suppress CCL20 and showed a trend toward suppression of IL‐33, CCL17 and eotaxin levels in lung tissue.DiscussionTaken together, these data show that purified DerP1/2‐SCIT is able to not only suppress AHR and inflammation, but also has superior activity toward suppression of Th2 cells and HDM‐induced activation of lung structural cells including airway epithelium.ConclusionsWe postulate that treatment with purified natural major allergens derived from HDM will likely increase clinical efficacy of SCIT.

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International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis

Wise

Lin

Toskala

et al. 2018

Int Forum Allergy Rhinol

339

453

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This critical review of the AR literature has identified several strengths; providers can be confident that treatment decisions are supported by rigorous studies. However, there are also substantial gaps in the AR literature. These knowledge gaps should be viewed as opportunities for improvement, as often the things that we teach and the medicine that we practice are not based on the best quality evidence. This document aims to highlight the strengths and weaknesses of the AR literature to identify areas for future AR research and improved understanding.

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The composition of house dust mite is critical for mucosal barrier dysfunction and allergic sensitisation

Cited by 137 publications

References 37 publications

Basal Cells Contribute to Innate Immunity of the Airway Epithelium through Production of the Antimicrobial Protein RNase 7

Basal Cells Contribute to Innate Immunity of the Airway Epithelium through Production of the Antimicrobial Protein RNase 7

Subcutaneous immunotherapy with purified Der p1 and 2 suppresses type 2 immunity in a murine asthma model

International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis

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