The Concentration of Oestrogens in Maternal Peripheral Blood During and After Labour

Roy, Eveline J.; Harkness, R. A.; Kerr, M. G.

doi:10.1111/j.1471-0528.1963.tb15048.x

Cited by 25 publications

(9 citation statements)

References 10 publications

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“…The values obtained during labour are similar to those reported by Jewelewicz, Bassett & Levitz (1969) and Rado, Deans Crystle & Townsley (1970). Roy et al (1963) observed a good correlation between blood and urinary oestriol levels. We obtained a good degree of correlation between plasma and urinary oestriol values in 41 matched analyses from five patients.…”

Section: Normal Pregnancy and Laboursupporting

confidence: 90%

“…In order to over¬ come these problems, oestriol determinations in amniotic fluid have been advocated (Schindler & Herrmann, 1966;Biggs, Klopper & Wilson, 1969), but even this pro¬ cedure has a limited value since the volume of liquor is variable in pregnancy and repeated sampling is difficult and often inconvenient to the patients. Measurements of plasma oestriol may be used to monitor the status of the foetus (Roy, Harkness & Kerr, 1963); Nachtigall/Bassett, Hogsander, Slagle & Levitz (1966) used fluorimetrie techniques for quantitative measurement of oestriol. Gas-liquid Chromatographie (g.l.c.)…”

Section: Introductionmentioning

confidence: 99%

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Determination of Plasma Oestriol in Normal Pregnancy and Labour Using Gas-Liquid Chromatography

Coyle¹,

Mills²

1971

Journal of Endocrinology

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A method is described for estimating oestriol in 2-10 ml samples of human pregnancy peripheral plasma. It incorporates acid hydrolysis, chemical purification, methylation, chromatography on alumina columns, formation of a derivative and quantitative determination by gas chromatography. A radioactive internal standard was added to correct for procedural losses. Plasma oestriol determinations in five normal patients throughout pregnancy and delivery are reported.

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Section: Normal Pregnancy and Laboursupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Determination of Plasma Oestriol in Normal Pregnancy and Labour Using Gas-Liquid Chromatography

Coyle¹,

Mills²

1971

Journal of Endocrinology

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“…E2 also participates in activation of the hypothalamo-hypophyso-adrenal (HPA) axis [5,6] as well as in maturation of cutaneous barrier [7,8]. It is also well documented that maternal blood levels of estrogens rise continuously during all pregnancy [9-11]. However, the level of E2 in the umbilical vein supplying oxygenated blood to the foetus does not increase in parallel with that in the maternal vein, whereas the level of E1 in the umbilical vein continues to increase during pregnancy [12,13].…”

Section: Introductionmentioning

confidence: 99%

Human type 2 17beta-hydroxysteroid dehydrogenase mRNA and protein distribution in placental villi at mid and term pregnancy

Drolet

Simard

Plante

et al. 2007

Reprod Biol Endocrinol

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BackgroundDuring human pregnancy, the placental villi produces high amounts of estradiol. This steroid is secreted by the syncytium, which is directly in contact with maternal blood. Estradiol has to cross placental foetal vessels to reach foetal circulation. The enzyme 17beta-hydroxysteroid dehydrogenase type 2 (17beta-HSD2) was detected in placental endothelial cells of foetal vessels inside the villi. This enzyme catalyzes the conversion of estradiol to estrone, and of testosterone to androstenedione. It was proposed that estradiol level into foetal circulation could be regulated by 17beta-HSD2.MethodsWe obtained placentas from 10 to 26 6/7 weeks of pregnancy from women undergoing voluntary termination of pregnancy, term placentas were collected after normal spontaneous vaginal deliveries. We quantified 17beta-HSD2 mRNA levels in mid-gestation and term human placenta by RT-QPCR. We produced a new anti-17beta-HSD2 antibody to study its spatio-temporal expression by immunohistochemistry. We also compared steroid levels (testosterone, estrone and estradiol) and 17beta-HSD2 mRNA and protein levels between term placenta and endometrium.ResultsHigh 17beta-HSD2 mRNA and protein levels were found in both mid-gestation and term placentas. However, we showed that 17beta-HSD2 mRNA levels increase by 2.27 fold between mid-gestation and term. This period coincides with a transitional phase in the development of the villous vasculature. In mid-gestation placenta, high levels of 17beta-HSD2 were found in mesenchymal villi and immature intermediate villi, more precisely in endothelial cells of the stromal channel. At term, high levels of 17beta-HSD2 were found in the numerous sinusoidal capillaries of terminal villi. 17beta-HSD2 mRNA and protein levels in term placentas were respectively 25.4 fold and 30 to 60 fold higher than in the endometrium. Steroid levels were also significantly higher in term placenta than in the endometrium.ConclusionThe spatial and temporal expression of 17beta-HSD2 in the placenta during pregnancy and the comparison of 17beta-HSD2 expression and steroid levels between placental villi and endometrium are compatible with a role in the modulation of active and inactive forms of estrogens. Our observations strongly support the hypothesis that 17beta-HSD2 acts as a barrier decreasing estradiol secretion rates in the foetal circulation.

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“…Many published reports show that the measurement of urinary oestriol in pregnancy reflects fetal growth and development. Far less information is available about maternal plasma oestriol levels-partly because the assay has been too time-consuming and also because the amount of plasma required was so large that repeated determinations could not be performed (Roy and Harkness, 1963;Smith and Hagerman, 1965;Nachtigall et al, 1966). The maternal plasma concentration of oestriol may be a more practical and reliable index of the function of the feto-placental unit than the measurement of oestriol in 24-hour and 48-hour samples of urine (Nachtigall et al, 1968;Ratanasopa et al, 1967) because repeat determinations can be carried out immediately, and a decrease in oestriol is apparent one day earlier in blood than in urine (Sandlberg and Slaunwhite, 1965).…”

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confidence: 99%

Plasma Oestriol in Pregnancy

Macourt

Corker²,

Naftolin³

1971

BJOG

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Summary Total plasma oestriol in pregnancy was estimated by a competitive protein binding method. The advantages of the measurement of plasma oestriol levels over the 24‐ or 48‐hour urinary oestriol assays are discussed. The pattern of plasma oestriol levels in the third trimester of pregnancy was investigated in over 400 healthy women and in general a uniform increase was found from the 28th week until term. Diurnal variation was found to be present, but this did not preclude the use of the method for assessing the feto‐placental unit. Exercise and meals had a minimal effect on the oestriol levels. Following delivery the level decreased sharply to reach, within four hours, one‐half that found just prior to delivery.

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The Concentration of Oestrogens in Maternal Peripheral Blood During and After Labour

Cited by 25 publications

References 10 publications

Determination of Plasma Oestriol in Normal Pregnancy and Labour Using Gas-Liquid Chromatography

Determination of Plasma Oestriol in Normal Pregnancy and Labour Using Gas-Liquid Chromatography

Human type 2 17beta-hydroxysteroid dehydrogenase mRNA and protein distribution in placental villi at mid and term pregnancy

Plasma Oestriol in Pregnancy

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