Using the questionnaire method on a sample of 92 Russian-speaking employees of the “person-person” professions type, the relationship of work alienation with personal characteristics was investigated. It was found that work alienation has associations with some personality traits. Also, the satisfaction of basic psychological needs at work is a significant predictor of work alienation. The study proved that work alienation is a relatively flexible construct for changes in the workplace that relate to autonomy, competence, and social relations. The results of the research can be used as an empirical justification of the model of the phenomenon as well as in the development of a differentiated approach to the prevention and intervention of work alienation, depending on the personality characteristics of employees.