The conserved cysteine-rich domain of a tesmin/TSO1-like protein binds zinc in vitro and TSO1 is required for both male and female fertility in Arabidopsis thaliana

Andersen, Stig Uggerhøj; Algreen-Petersen, Randi Groslier; Hoedl, Martina; Jurkiewicz, Anna; Cvitanich, Cristina; Braunschweig, Ulrich; Schauser, Leif; Oh, Sung‐Aeong; Twell, David; Jensen, Erik Appel

doi:10.1093/jxb/erm215

Cited by 66 publications

(76 citation statements)

References 43 publications

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“…Subsequent work has shown this domain to be important for the function of E(z) and E(z)-like proteins (Carrington and Jones, 1996;Kuzmichev et al, 2002;Ketel et al, 2005). Several otherwise unrelated proteins from plants and animals have been described that contain CXC domains separated by sequences of varying length, and these domains have been shown to bind zinc in vitro (Andersen et al, 2007). TSO1 is a CXC-containing protein in Arabidopsis that functions in the regulation of cell division, and both the tso1-1 and tso1-2 loss-of-function alleles contain amino acid substitutions within the CXC domain (Hauser et al, 2000;Song et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

A Single Amino Acid Change in the Enhancer of Zeste Ortholog CURLY LEAF Results in Vernalization-Independent, Rapid Flowering in Arabidopsis

Doyle

Amasino

2009

Plant Physiology

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Many strains of Arabidopsis (Arabidopsis thaliana) require exposure to prolonged cold for rapid flowering, a process known as vernalization. Vernalization in Arabidopsis results in the suppression of FLOWERING LOCUS C (FLC), a repressor of flowering. In a screen for mutants that no longer require vernalization for rapid flowering, we identified a dominant allele of the Enhancer of Zeste E(z) ortholog CURLY LEAF (CLF), clf-59. CLF is a Polycomb Group gene, and the clf-59 mutant protein contains a proline-to-serine transition in a cysteine-rich region that precedes the SET domain. Mutant plants are early flowering and have reduced FLC expression, but, unlike clf loss-of-function mutants, clf-59 mutants do not display additional pleiotropic phenotypes. clf-59 mutants have elevated levels of trimethylation on lysine 27 of histone H3 (H3K27me3) at FLC. Thus, clf-59 appears to be a gain-of-function allele, and this allele represses FLC without some of the components required for vernalization-mediated repression. In the course of this work, we also identified a marked difference in H3K27me3 levels at FLC between plants that contain and those that lack the FRIGIDA (FRI) gene. Furthermore, FRI appears to affect CLF occupancy at FLC; thus, our work provides insight into the molecular role that FRI plays in delaying the onset of flowering.

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Section: Discussionmentioning

confidence: 99%

A Single Amino Acid Change in the Enhancer of Zeste Ortholog CURLY LEAF Results in Vernalization-Independent, Rapid Flowering in Arabidopsis

Doyle

Amasino

2009

Plant Physiology

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“…2B). TSO1 and other CXC proteins, e.g., soybean CPP1 (18), human LIN54 (19), and Caenorhabditis elegans Lin54 (20), have been shown to bind zinc (21) and specific DNA sequences through their CXC domains. To determine whether CHT7 is located in the nucleus, we added a C-terminal green fluorescent protein (GFP) to CHT7, thereby increasing its size by 30 kDa, and introduced it into the cht7 background (Fig.…”

Section: Significancementioning

confidence: 99%

The protein Compromised Hydrolysis of Triacylglycerols 7 (CHT7) acts as a repressor of cellular quiescence in Chlamydomonas

Tsai

Warakanont

Takeuchi

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

107

116

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Microalgae are prolific photosynthetic organisms that have the potential to sustainably produce high-value chemical feedstocks. However, an industry based on microalgal biomass still is faced with challenges. For example, microalgae tend to accumulate valuable compounds, such as triacylglycerols, only under stress conditions that limit growth. To investigate the fundamental mechanisms at the base of this conundrum-the inverse relationship between biomass production and storage compound accumulation-we applied a combination of cell biological and genetic approaches. Conceptually, nutrient deprivation, which commonly is used to induce the accumulation of triacylglycerol in microalgae, leads to a state of cellular quiescence defined by a halt of cell divisions that is reversible upon nutrient resupply. To identify factors that govern cellular quiescence, we screened for mutants of the model alga Chlamydomonas reinhardtii that, in contrast to wildtype cells placed under conditions of nitrogen deprivation, were unable to degrade triacylglycerols following nitrogen resupply. One of the mutants described here in detail, compromised hydrolysis of triacylglycerols 7 (cht7), was severely impaired in regrowth following removal of different conditions inducing cellular quiescence. The mutant carries a deletion affecting four genes, only one of which rescued the quiescence phenotype when reintroduced. It encodes a protein with similarity to mammalian and plant DNA binding proteins. Comparison of transcriptomes indicated a partial derepression of quiescence-related transcriptional programs in the mutant under conditions favorable to growth. Thus, CHT7 likely is a repressor of cellular quiescence and provides a possible target for the engineering of high-biomass/high-triacylglycerol microalgae.algae | lipid metabolism | nutrient stress | cellular quiescence | transcriptome N utrient deprivation of microalgal cultures provides a facile experimental tool to induce and study triacylglycerol (TAG) accumulation in lipid droplets and is used in biotechnological settings for the production of high-value oils (1, 2). In particular, responses to the withdrawal of nitrogen (N) have been studied widely in the model unicellular green alga Chlamydomonas reinhardtii, and a comprehensive picture of N-sparing mechanisms during N deprivation is emerging through integrated global analysis of transcripts, proteins, and metabolites (3-5). Mechanisms of lipid droplet formation following N deprivation and proteins associated with lipid droplets are being explored (6-8), and mutants have become available that provide mechanistic insights in vivo into specific aspects of the lipid biosynthetic machinery of C. reinhardtii required for TAG accumulation (9-11).From a cell biological viewpoint, N deprivation induces cellular quiescence, a reversible state of the cell cycle during which cell divisions temporarily cease and cells are reprogrammed to adjust metabolism for survival of the adverse condition (12). In C. reinhardtii, metabolic changes during N ...

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“…CPP transcription factors are a small gene family and the typical sequence consists of 1 or 2 cysteine-rich domains, known as CXC domains. CXC domains and the sequences connecting them are highly conserved in nearly all species (Riechmann et al, 2000;Andersen et al, 2007). CPP transcription factors have been found to have various functions in many plant species.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of the CPP-like gene family in soybean

Zhang¹,

Zhao²,

Wang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Cysteine-rich polycomb-like protein (CPP-like) genes are a group of transcription factors with highly conserved cysteinerich domains and are widely distributed in animals and plants, but do not present in yeast. Previous studies have shown that members of this family play important roles in the development of reproductive tissue and in the control of cell division in plants. In this study, whole genome identification of soybean CPP transcription factors was performed using bioinformatic methods. The results showed that there were 20 CPP transcription factors in the soybean genome, In silico analysis of soybean CPP-like gene family which encoded for 28 distinct CPP proteins. These transcription factors were distributed on 16 of 20 chromosomes. Phylogenetic relationship analysis showed that expression of CPP gene family members occurred before the differentiation of monocotyledons and dicotyledons. RNA-Seq analysis showed that 5 genes were highly expressed in all tissues, including Glyma10g39080, Glyma01g44670, Glyma101g66920, Glyma02g01540, and Glyma20g28740. One gene (Glyma14g14750) was specifically expressed in young leaves, while 2 genes (Glyma02g01540 and Glyma10g01580) were highly expressed in root nodules. Quantitative reverse transcriptasepolymerase chain reaction analysis revealed that the expression levels of most genes increased in the roots under high temperature stress. Our findings indicate that these genes are not only involved in growth and development, but also in the responses to high temperature stress in soybean roots.

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The conserved cysteine-rich domain of a tesmin/TSO1-like protein binds zinc in vitro and TSO1 is required for both male and female fertility in Arabidopsis thaliana

Cited by 66 publications

References 43 publications

A Single Amino Acid Change in the Enhancer of Zeste Ortholog CURLY LEAF Results in Vernalization-Independent, Rapid Flowering in Arabidopsis

A Single Amino Acid Change in the Enhancer of Zeste Ortholog CURLY LEAF Results in Vernalization-Independent, Rapid Flowering in Arabidopsis

The protein Compromised Hydrolysis of Triacylglycerols 7 (CHT7) acts as a repressor of cellular quiescence in Chlamydomonas

Genome-wide identification and expression analysis of the CPP-like gene family in soybean

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