2023
DOI: 10.1016/j.vetmic.2022.109619
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The conserved L1089 in the S2 subunit of avian infectious bronchitis virus determines viral kidney tropism by disrupting virus-cell fusion

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Cited by 5 publications
(5 citation statements)
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“…Despite the mutation to rare amino acids in rSczy3S2 -G543S-Q544S-S553T-F557Y at the cleavage site in the front part of the protein, this process may cause a decrease in the performance of the S2 protein and result in a decrease in the formation of syncytia. The mutations G543S, Q544S, S553T, and F557Y, which are all from the non-adapted strain CN of CEKs and are situated near the S1/S2 cleavage site, might be responsible for this activity [ 5 ]. The mutations of S2 subunit change its conformation or function, which could impede the activity of ABI1 and NCKAP1 pathway, likely causing the lessening of syncytia ( Figure 5 B).…”
Section: Discussionmentioning
confidence: 99%
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“…Despite the mutation to rare amino acids in rSczy3S2 -G543S-Q544S-S553T-F557Y at the cleavage site in the front part of the protein, this process may cause a decrease in the performance of the S2 protein and result in a decrease in the formation of syncytia. The mutations G543S, Q544S, S553T, and F557Y, which are all from the non-adapted strain CN of CEKs and are situated near the S1/S2 cleavage site, might be responsible for this activity [ 5 ]. The mutations of S2 subunit change its conformation or function, which could impede the activity of ABI1 and NCKAP1 pathway, likely causing the lessening of syncytia ( Figure 5 B).…”
Section: Discussionmentioning
confidence: 99%
“…As previously mentioned, CN is a type of infectious bronchitis virus (IBV) that is unable to infect CEKs. Following a sequence assessment and investigation, CN strain of IBV was used as the benchmark for the mutations G543S, Q544S, S553T, and F557Y in rSczy3, leading to the generation of rSczy3S2 -G543S-Q544S-S553T-F557Y (TCID 50 /mL = 10 3.5 ) [ 5 ]. Successive passages of Sczy3 with CEKs 100 times led to the creation of zy100, which is a variant of zy30 with a distinct S2 subunit that could generate bigger and greater numbers of syncytia when infected with CEKs.…”
Section: Methodsmentioning
confidence: 99%
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“…The S2 subunit is a highly conserved part formed primarily by the heptad repeat regions, HRP1 and HRP2, and a fusion peptide that is responsible mainly for the viral entry and membrane fusion [48]. Research has shown that the S2 subunit is responsible for viral adaptation to various cell lines [52][53][54][55][56][57][58]. The S2 subunit also contains some minor neutralizing epitopes and contributes to the avidity and specificity of virus attachment, and thus viral host range [51].…”
Section: Infectious Bronchitis Virusmentioning
confidence: 99%